LOCUS Exported 7824 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCMV4-p105 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7824) AUTHORS Beraud C, Sun SC, Ganchi P, Ballard DW, Greene WC TITLE Human T-cell leukemia virus type I Tax associates with and is negatively regulated by the NF-kappa B2 p100 gene product: implications for viral latency. JOURNAL Mol Cell Biol. 1994 Feb . 14(2):1374-82. PUBMED 8289813 REFERENCE 2 (bases 1 to 7824) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7824 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 1..17 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 178..557 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 558..761 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 712..732 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind complement(4051..4070) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" primer_bind complement(4498..4518) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4503..4832 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4683..4818 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4745..4764 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 4871..4967 /label=SV40 intron /note="modified SV40 intron with splice donor and acceptor sites" primer_bind complement(5043..5062) /label=T7 /note="T7 promoter, forward primer" promoter complement(5044..5062) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5076..5092) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5076..5092) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5089..5111) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5100..5116 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5124..5154) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5169..5190 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5307..5324) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5478..6066) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5558..5577) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6237..7097) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6860..6879 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7098..7202) /gene="bla" /label=AmpR promoter rep_origin 7229..7684 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7316..7335) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7526..7547 /label=F1ori-F /note="F1 origin, forward primer" primer_bind 7810..8 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 7824..17 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" ORIGIN 1 gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca 61 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt 121 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac 181 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 421 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 481 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag 541 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt 601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 661 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg 721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga 781 attgttttta tttttaattt tctttcaaat acttccatcg aattcagatc tgcttaggag 841 ggagagccca cccgcgccag gaggccgaac gcggactcgc cacccggctt cagaatggca 901 gaagatgatc catatttggg aaggcctgaa caaatgtttc atttggatcc ttctttgact 961 catacaatat ttaatccaga agtatttcaa ccacagatgg cactgccaac agcagatggc 1021 ccataccttc aaatattaga gcaacctaaa cagagaggat ttcgtttccg ttatgtatgt 1081 gaaggcccat cccatggtgg actacctggt gcctctagtg aaaagaacaa gaagtcttac 1141 cctcaggtca aaatctgcaa ctatgtggga ccagcaaagg ttattgttca gttggtcaca 1201 aatggaaaaa atatccacct gcatgcccac agcctggtgg gaaaacactg tgaggatggg 1261 atctgcactg taactgctgg acccaaggac atggtggtcg gcttcgcaaa cctgggtata 1321 cttcatgtga caaagaaaaa agtatttgaa acactggaag cacgaatgac agaggcgtgt 1381 ataaggggct ataatcctgg actcttggtg caccctgacc ttgcctattt gcaagcagaa 1441 ggtggagggg accggcagct gggagatcgg gaaaaagagc taatccgcca agcagctctg 1501 cagcagacca aggagatgga cctcagcgtg gtgcggctca tgtttacagc ttttcttccg 1561 gatagcactg gcagcttcac aaggcgcctg gaacccgtgg tatcagacgc catctatgac 1621 agtaaagccc ccaatgcatc caacttgaaa attgtaagaa tggacaggac agctggatgt 1681 gtgactggag gggaggaaat ttatcttctt tgtgacaaag ttcagaaaga tgacatccag 1741 attcgatttt atgaagagga agaaaatggt ggagtctggg aaggatttgg agatttttcc 1801 cccacagatg ttcatagaca atttgccatt gtcttcaaaa ctccaaagta taaagatatt 1861 aatattacaa aaccagcctc tgtgtttgtc cagcttcgga ggaaatctga cttggaaact 1921 agtgaaccaa aacctttcct ctactatcct gaaatcaaag ataaagaaga agtgcagagg 1981 aaacgtcaga agctcatgcc caatttttcg gatagtttcg gcggtggtag tggtgccgga 2041 gctggaggcg gaggcatgtt tggtagtggc ggtggaggag ggggcactgg aagtacaggt 2101 ccagggtata gcttcccaca ctatggattt cctacttatg gtgggattac tttccatcct 2161 ggaactacta aatctaatgc tgggatgaag catggaacca tggacactga atctaaaaag 2221 gaccctgaag gttgtgacaa aagtgatgac aaaaacactg taaacctctt tgggaaagtt 2281 attgaaacca cagagcaaga tcaggagccc agcgaggcca ccgttgggaa tggtgaggtc 2341 actctaacgt atgcaacagg aacaaaagaa gagagtgctg gagttcagga taacctcttt 2401 ctagagaagg ctatgcagct tgcaaagagg catgccaatg cccttttcga ctacgcggtg 2461 acaggagacg tgaagatgct gctggccgtc cagcgccatc tcactgctgt gcaggatgag 2521 aatggggaca gtgtcttaca cttagcaatc atccaccttc attctcaact tgtgagggat 2581 ctactagaag tcacatctgg tttgatttct gatgacatta tcaacatgag aaatgatctg 2641 taccagacgc ccttgcactt ggcagtgatc actaagcagg aagatgtggt ggaggatttg 2701 ctgagggctg gggccgacct gagccttctg gaccgcttgg gtaactctgt tttgcaccta 2761 gctgccaaag aaggacatga taaagttctc agtatcttac tcaagcacaa aaaggcagca 2821 ctacttcttg accaccccaa cggggacggt ctgaatgcca ttcatctagc catgatgagc 2881 aatagcctgc catgtttgct gctgctggtg gccgctgggg ctgacgtcaa tgctcaggag 2941 cagaagtccg ggcgcacagc actgcacctg gctgtggagc acgacaacat ctcattggca 3001 ggctgcctgc tcctggaggg tgatgcccat gtggacagta ctacctacga tggaaccaca 3061 cccctgcata tagcagctgg gagagggtcc accaggctgg cagctcttct caaagcagca 3121 ggagcagatc ccctggtgga gaactttgag cctctctatg acctggatga ctcttgggaa 3181 aatgcaggag aggatgaagg agttgtgcct ggaaccacgc ctctagatat ggccaccagc 3241 tggcaggtat ttgacatatt aaatgggaaa ccatatgagc cagagtttac atctgatgat 3301 ttactagcac aaggagacat gaaacagctg gctgaagatg tgaagctgca gctgtataag 3361 ttactagaaa ttcctgatcc agacaaaaac tgggctactc tggcgcagaa attaggtctg 3421 gggatactta ataatgcctt ccggctgagt cctgctcctt ccaaaacact tatggacaac 3481 tatgaggtct ctgggggtac agtcagagag ctggtggagg ccctgagaca aatgggctac 3541 accgaagcaa ttgaagtgat ccaggcagcc tccagcccag tgaagaccac ctctcaggcc 3601 cactcgctgc ctctctcgcc tgcctccaca aggcagcaaa tagacgagct ccgagacagt 3661 gacagtgtct gcgacagcgg cgtggagaca tccttccgca aactcagctt taccgagtct 3721 ctgaccagtg gtgcctcact gctaactctc aacaaaatgc cccatgatta tgggcaggaa 3781 ggacctctag aaggcaaaat ttagaagctt gcatgcctgc aggtcgactc tagaggatcc 3841 ttaagttaac ttaatggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag 3901 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg 3961 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag 4021 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg 4081 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc 4141 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt 4201 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga 4261 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc 4321 ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca cagcataggc 4381 tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact gtcctctcat 4441 gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagct tggctgtgga 4501 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 4561 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 4621 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 4681 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 4741 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 4801 gaggcttttt tggaggccta ggcttttgca aaaagctcct cgaggaactg aaaaaccaga 4861 aagttaactg gtaagtttag tctttttgtc ttttatttca ggtcccggat ccggtggtgg 4921 tgcaaatcaa agaactgctc ctcagtggat gttgccttta cttctaggcc tgtacggaag 4981 tgttacttct gctctaaaag ctgctgcagg tcgactctag ggccgcgtac cgagctcgaa 5041 ttccctatag tgagtcgtat taaattcgta atcatgtcat agctgtttcc tgtgtgaaat 5101 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 5161 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 5221 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 5281 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 5341 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 5401 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 5461 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 5521 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 5581 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 5641 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 5701 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 5761 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 5821 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 5881 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 5941 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 6001 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 6061 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 6121 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 6181 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 6241 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 6301 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 6361 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 6421 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 6481 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 6541 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 6601 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 6661 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 6721 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 6781 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 6841 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 6901 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 6961 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 7021 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 7081 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 7141 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 7201 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg 7261 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 7321 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 7381 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 7441 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 7501 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 7561 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 7621 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca 7681 atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt 7741 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc 7801 cagggttttc ccagtcacga cgtt //