LOCUS Exported 10595 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cre/Lox C-terminal tagging construct encoding glutathione S-transferase. ACCESSION . VERSION . KEYWORDS pAW8_GST SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10595) AUTHORS Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J. TITLE Gene tagging and gene replacement using recombinase-mediated cassette exchange in Schizosaccharomyces pombe. JOURNAL Gene. 2008 Jan 15;407(1-2):63-74 PUBMED 18054176 REFERENCE 2 (bases 1 to 10595) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10595 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 575..1356 /label=ars1 /note="Schizosaccharomyces pombe autonomously replicating sequence ars1" primer_bind complement(1451..1470) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 1570..1592 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(1630..1648) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1716..1820 /gene="bla" /label=AmpR promoter CDS 1821..2681 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2039..2058) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2852..3440 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3341..3360 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3594..3611 /label=L4440 /note="L4440 vector, forward primer" protein_bind 3728..3749 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 3764..3794 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3802..3818 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 3807..3829 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 3826..3842 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 3826..3842 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" protein_bind 3874..3907 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS 3916..4569 /codon_start=1 /product="glutathione S-transferase from Schistosoma japonicum" /label=GST /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK" primer_bind 4527..4549 /label=pGEX 5' /note="pGEX vectors, Glutathione-S-transferase, forward primer" terminator 4617..4804 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(4672..4696) /label=pHybLex-R /note="ADH terminator, reverse primer" gene 4879..6235 /label=kanMX /note="yeast selectable marker conferring kanamycin resistance (Wach et al., 1994)" promoter 4879..5222 /label=TEF promoter /note="Ashbya gossypii TEF promoter" CDS 5223..6032 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(5290..5309) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 6038..6235 /label=TEF terminator /note="Ashbya gossypii TEF terminator" primer_bind complement(6329..6346) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(6329..6345) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6338..6360) /label=M13/pUC Forward /note="In lacZ gene" CDS 9244..10338 /codon_start=1 /gene="S. cerevisiae LEU2" /product="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /label=LEU2 /note="yeast auxotrophic marker" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" ORIGIN 1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg 61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat 121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa 181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc 241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag 301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc 361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt 421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa 481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg 541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat 601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt 661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag 721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac 781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg 841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt 901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc 961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta 1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt 1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca 1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat 1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt 1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact 1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt 1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt 1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2581 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac 2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg 3901 aagttatgca tgctcatgtc ccctatacta ggttattgga aaattaaggg ccttgtgcaa 3961 cccactcgac ttcttttgga atatcttgaa gaaaaatatg aagagcattt gtatgagcgc 4021 gatgaaggtg ataaatggcg aaacaaaaag tttgaattgg gtttggagtt tcccaatctt 4081 ccttattata ttgatggtga tgttaaatta acacagtcta tggccatcat acgttatata 4141 gctgacaagc acaacatgtt gggtggttgt ccaaaagagc gtgcagagat ttcaatgctt 4201 gaaggagcgg ttttggatat tagatacggt gtttcgagaa ttgcatatag taaagacttt 4261 gaaactctca aagttgattt tcttagcaag ctacctgaaa tgctgaaaat gttcgaagat 4321 cgtttatgtc ataaaacata tttaaatggt gatcatgtaa cccatcctga cttcatgttg 4381 tatgacgctc ttgatgttgt tttatacatg gacccaatgt gcctggatgc gttcccaaaa 4441 ttagtttgtt ttaaaaaacg tattgaagct atcccacaaa ttgataagta cttgaaatcc 4501 agcaagtata tagcatggcc tttgcagggc tggcaagcca cgtttggtgg tggcgaccat 4561 cctccaaaat cggatctggt tccgcgttga ggcgcgccag atctacttct aaataagcga 4621 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca 4681 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc 4741 tgtaggtcag gttgctttct caggtatagt atgaggtcgc tcttattgac cacacctcta 4801 ccggcagatc cgctagggat aacagggtaa tatagatctg tttagcttgc ctcgtccccg 4861 ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg 4921 cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta 4981 taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc 5041 tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac 5101 gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat 5161 atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa 5221 ccatgggtaa ggaaaagact cacgtttcga ggccgcgatt aaattccaac atggatgctg 5281 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc 5341 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg 5401 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc 5461 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc 5521 ccggcaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg 5581 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta 5641 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg 5701 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa 5761 tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg 5821 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa 5881 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt 5941 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc 6001 agtttcattt gatgctcgat gagtttttct aatcagtact gacaataaaa agattcttgt 6061 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa 6121 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag 6181 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc 6241 gattcgatac taacgccgcc atccagtcga cgaactagta taacttcgta tatggtatta 6301 tatacgaagt tatgcggccg ctgaattcac tggccgtcgt tttacaacgt cgtgactggg 6361 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc 6421 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 6481 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac 6541 catcgtccgg atcaatgaag tagatgagtt tactacctgt atatactttt ttttttgtca 6601 aagtcacttt tatggcattt ccatgttgaa ggattagcca cagcactaaa ttttcagcgt 6661 atgattgctt ttaaatattt aattttcatc gttttttaat taatattcaa aacgatttaa 6721 tgccttctac atctgaagtc aagctcatag actcgggttg ggtagctaaa actacatcta 6781 acacgaaggg gtatagcctt cttaaagtat actcatgtat aaacgttttg ggttcatatt 6841 tttgggccac gtggtttata aaaattctta actacaccac tcggtatccc gcacccgtct 6901 acgtttctac gatttcgagc taatattgag taaagtgatt agcaaaaaaa ataaaagtac 6961 tcgttgtcgg agatcaagaa tttttctatt atctcatcta aaccactttc taaaagcgaa 7021 aaacaaaatc gtaatatgca gcttgaatgg gcttccatag tttgaaagaa aaaccctagc 7081 agtactggca agggagacat tccttttacc tggctaatcg ccatcttcca gcaggcgcac 7141 cattgcccct gtttcactat ccaggttacg gatatagttc atgacaatat ttacattggt 7201 ccagccacca gcttgcatga tctccggtat tgaaactcca gcgcgggcca tatctcgcgc 7261 ggctccgaca cgggcactgt gtccagacca ggccaggtat ctctgaccag agtcatcctt 7321 agcgccgtaa atcaatcgat gagttgcttc aaaaatccct tccagggcgc gagttgatag 7381 ctggctggtg gcagatggcg cggcaacacc attttttctg acccggcaaa acaggtagtt 7441 attcggatca tcagctacac cagagacgga aatccatcgc tcgaccagtt tagttacccc 7501 caggctaagt gccttctcta cacctgcggt gctaaccagc gttttcgttc tgccaatatg 7561 gattaacatt ctcccaccgt cagtacgtga gatatcttta accctgatcc tggcaatttc 7621 ggctatacgt aacagggtgt tataagcaat ccccagaaat gccagattac gtatatcctg 7681 gcagcgatcg ctattttcca tgagtgaacg aacctggtcg aaatcagtgc gttcgaacgc 7741 tagagcctgt tttgcacgtt caccggcatc aacgttttct tttcggatcc gccgcataac 7801 cagtgaaaca gcattgctgt cacttggtcg tggcagcccg gaccgacgat gaagcatgtt 7861 tagctggccc aaatgttgct ggatagtttt tactgccaga ccgcgcgcct gaagatatag 7921 aagataatcg cgaacatctt caggttctgc gggaaaccat ttccggttat tcaacttgca 7981 ccatgccgcc cacgaccggc aaacggacag aagcattttc caggtatgct cagaaaacgc 8041 ctggcgatcc ctgaacatgt ccatcaggtt cttgcgaacc tcatcactcg ttgcatcgac 8101 cggtaacttt tagattaaca tttgtttaaa tatgaaatta ggcaatgtaa catactgcag 8161 gcaaattttg gtgtacggta agtaaattgg acatatgatt taacaaagcg actataagtc 8221 agaaagtgag aatgagattg aaataattaa ttcaacttat tcaattgatg atatgccagg 8281 attcctcttc ctttatatat cttcaatgtt tcgattaaca ggtccattat ccggttttta 8341 gtttcgctgc acattgccga atgacaatcg gattccttta agctttgctt ttgccctgtt 8401 ttctctcccc agtaatcgtt gggtgactga accatccagt agccgagagt tacgtttttc 8461 ctgatttaat aaatatccat atctatttgt ctcgtggtgt tttacagcaa ctaaccgaaa 8521 ttactatttt gcatcatcca accataaaga gacaacaata attctagaaa acatataaca 8581 agtgtttttt tctatttacg tctcatacgc aaagtccact ttaccatgtt ttggaaactt 8641 gtacccaacc attcattttt cgattttgta atgaggctct tttcttctaa caatcattga 8701 ggtcattatc atgtgtcctc acctattgtt taaattgagg cttgcgctat tcccaaaatt 8761 gtgggtatta ttttcatata tcagtatctg caatttcggt tcaactccgg aagctcggta 8821 cccaactgtg ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca 8881 ttattttttt tctctcaaca taacgagaac acacaggggc gctatcgcac agaatcaaat 8941 tcgatgactg gaaatttttt gttaatttca gaggtcgcct gacgcatata cctttttcaa 9001 ctgaaaaatt gggagaaaaa ggaaaggtga gagcgccgga accggctttt catatagaat 9061 agagaagcgt tcatgactaa atgcttgcat cacaatactt gaagttgaca atattattta 9121 aggacctatt gttttttcca ataggtggtt agcaatcgtc ttactttcta acttttctta 9181 ccttttacat ttcagcaata tatatatata tatatatata tatttcaagg atataccatt 9241 gtaatgtctg cccctaagaa gatcgtcgtt ttgccaggtg accacgttgg tcaagaaatc 9301 acagccgaag ccattaaggt tcttaaagct atttctgatg ttcgttccaa tgtcaagttc 9361 gatttcgaaa atcatttaat tggtggtgct gctatcgatg ctacaggtgt tccacttcca 9421 gatgaggcgc tggaagcctc caagaaggct gatgccgttt tgttaggtgc tgtgggtggt 9481 cctaaatggg gtaccggtag tgttagacct gaacaaggtt tactaaaaat ccgtaaagaa 9541 cttcaattgt acgccaactt aagaccatgt aactttgcat ccgactctct tttagactta 9601 tctccaatca agccacaatt tgctaaaggt actgacttcg ttgttgttag agaattagtg 9661 ggaggtattt actttggtaa gagaaaggaa gacgatggtg atggtgtcgc ttgggatagt 9721 gaacaataca ccgttccaga agtgcaaaga atcacaagaa tggccgcttt catggcccta 9781 caacatgagc caccattgcc tatttggtcc ttggataaag ctaatgtttt ggcctcttca 9841 agattatgga gaaaaactgt ggaggaaacc atcaagaacg aatttcctac attgaaagtt 9901 caacatcaat tgattgattc tgccgccatg atcctagtta agaacccaac ccacctaaat 9961 ggtattataa tcaccagcaa catgtttggt gatatcatct ccgatgaagc ctccgttatc 10021 ccaggctcct tgggtttgtt gccatctgcg tccttggcct ctttgccaga caagaacacc 10081 gcatttggtt tgtacgaacc atgccatggt tccgctccag atttgccaaa gaataaggtc 10141 aaccctatcg ccactatctt gtctgctgca atgatgttga aattgtcatt gaacttgcct 10201 gaagaaggta aagccattga agatgcagtt aaaaaggttt tggatgcagg tatcagaact 10261 ggtgatttag gtggttccaa cagtaccacc gaagtcggtg atgctgtcgc cgaagaagtt 10321 aagaaaatcc ttgcttaaaa agattctctt tttttgtgat atttgtacat aaactttata 10381 aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg gaatatgttc atagggtaga 10441 cgaaactata tacgcaatct acatacattt atcaagaagg agaaaaagga ggatgtaaag 10501 gaatacaggt aagcaaattg atactaatgg ctcaacgtga taaggaaaaa gaattgcact 10561 ttaacattaa tattgacaag gaggagggca tcaca //