LOCUS       Exported                8793 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses catalytically inactive dCas9 with N-terminal fusion of 
            Sin3a Interacting Domain (SID) and C-terminal fusion of repressive 
            KRAB domain.
ACCESSION   .
VERSION     .
KEYWORDS    SID4x-dCas9-KRAB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Carleton JB, Berrett KC, Gertz J
  TITLE     Multiplex Enhancer Interference Reveals Collaborative Control of 
            Gene Regulation by Estrogen Receptor alpha-Bound Enhancers.
  JOURNAL   Cell Syst. 2017 Oct 25;5(4):333-344.e5. doi: 
            10.1016/j.cels.2017.08.011. Epub 2017 Sep 27.
  PUBMED    28964699
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(109..903)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     enhancer        1097..1476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1477..1680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1631..1651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          1772..1904
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     protein_bind    1919..1943
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1958..1967
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2363..2389
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2393..2413
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2423..6523
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6527..6547
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6611..6805
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             6806..6871
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6893..6946
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6962..7765
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    complement(7781..7805)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7858..7899
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(7925..7944)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    7954..8035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     7979..7998
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8233..28)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8313..8332)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
       61 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgccgtctc agaagaactc
      121 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
      181 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
      241 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
      301 gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc
      361 gccgtcgggc atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
      421 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctctctc
      481 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
      541 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
      601 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
      661 gagtacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
      721 ttgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
      781 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
      841 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
      901 cataatatta ttgaagcatt tatcagggtt cgtctcgtcc cggtctcctc ccatgcatgt
      961 caatattggc cattagccat attattcatt ggttatatag cataaatcaa tattggctat
     1021 tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca
     1081 atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc aattacgggg
     1141 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     1201 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1261 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1321 cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac
     1381 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg
     1441 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc
     1501 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     1561 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc
     1621 gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagaggt
     1681 cgtttagtga accgtcagat cactagtagc tttattgcgg tagtttatca cagttaaatt
     1741 gctaacgcag tcagtgctcg actgatcaca ggtaagtatc aaggttacaa gacaggttta
     1801 aggaggccaa tagaaactgg gcttgtcgag acagagaaga ttcttgcgtt tctgataggc
     1861 acctattggt cttactgaca tccactttgc ctttctctcc acagggaaaa aacaattgac
     1921 aagtttgtac aaaaaagcag gctccgaatt caccggtgcc accatgggct ccggaatgaa
     1981 cattcagatg ttgctcgagg ctgctgatta cctggagcgc cgcgaacggg aagcggaaca
     2041 cggttacgct agtatgctgc ctgggagcgg catgaatatc cagatgctgc tcgaggcggc
     2101 cgattatctc gaacgcagag agagagaagc cgaacatggc tacgcctcta tgctgcctgg
     2161 ctccgggatg aatatacaga tgctcctgga agctgccgac tatctggagc gaagagaaag
     2221 ggaggctgag cacgggtatg caagtatgct ccctggctcc ggtatgaata tccagatgct
     2281 gctggaggcc gctgactatc ttgaacgccg agaaagggaa gccgagcatg gatatgcatc
     2341 catgctgcct tctaggtctg cctacccata cgatgttcca gattacgctt cgccgaagaa
     2401 aaagcgcaag gtcgaagcgt ccgacaagaa gtacagcatc ggcctggcca tcggcaccaa
     2461 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt
     2521 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga
     2581 cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag
     2641 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga
     2701 cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga
     2761 gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac
     2821 catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat
     2881 ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct
     2941 gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca
     3001 gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc
     3061 cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa
     3121 gaatggcctg ttcggcaacc tgattgccct gagcctgggc ctgaccccca acttcaagag
     3181 caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga
     3241 cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa
     3301 gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa
     3361 ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct
     3421 gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca
     3481 gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa
     3541 gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa
     3601 cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat
     3661 ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa
     3721 ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc
     3781 tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac
     3841 cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg
     3901 gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct
     3961 gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat
     4021 gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa
     4081 gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg
     4141 cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata
     4201 ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga
     4261 cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga
     4321 acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg
     4381 gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca
     4441 gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat
     4501 gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc
     4561 cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa
     4621 gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca
     4681 caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca
     4741 gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca
     4801 gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta
     4861 ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc
     4921 cgactacgat gtggacgcca tcgtgcctca gagctttctg aaggacgact ccatcgacaa
     4981 caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga
     5041 ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca
     5101 gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc
     5161 cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat
     5221 cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa
     5281 agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa
     5341 agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg
     5401 aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa
     5461 ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc
     5521 caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa
     5581 cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg
     5641 ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat
     5701 cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag
     5761 gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt
     5821 cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc
     5881 caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt
     5941 cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct
     6001 gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct
     6061 ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa
     6121 cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca
     6181 gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag
     6241 cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta
     6301 caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac
     6361 cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa
     6421 gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg
     6481 cctgtacgag acacggatcg acctgtctca gctgggaggc gacagcccca agaagaagag
     6541 aaaggtggag gccagcgggc cggccggatc cgggcgcgcc atggacgcga aatcacttac
     6601 ggcatggtcg agaacactgg ttacgttcaa ggacgtgttt gtggacttta cacgtgagga
     6661 gtggaaattg ctggatactg cgcaacaaat tgtgtatcga aatgtcatgc ttgagaatta
     6721 caagaacctc gtcagtctcg gataccagtt gacgaaaccg gatgtgatcc ttaggctcga
     6781 aaagggggaa gaaccttggc tggtagacta caaagaccat gacggtgatt ataaagatca
     6841 tgacatcgac tacaaggatg acgatgacaa aatcgataga tctggcagcg gagagggcag
     6901 aggaagcctt ctaacatgcg gtgacgtgga ggagaatccc ggccctaggc tcgagacgcg
     6961 tatgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     7021 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     7081 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     7141 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     7201 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     7261 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     7321 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     7381 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     7441 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     7501 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     7561 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     7621 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     7681 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     7741 tcgccttctt gacgagttct tctgattaat taagaattcg acccagcttt cttgtacaaa
     7801 gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt
     7861 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta gcaattgttt
     7921 tttcgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     7981 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     8041 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     8101 caagtaaaac ctctacaaat gtggtactta agagggggag accaaagggc gagacgttaa
     8161 ggcctcacgt gacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacg ggatacctgt ccgcctttct
     8401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8521 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8641 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8701 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8761 tggtagcggt ggtttttttg tttgcaagca gca
//
