LOCUS Exported 2892 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Bacterial expression of mutant human alpha synuclein. ACCESSION . VERSION . KEYWORDS pT7-7 asyn K96R SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2892) TITLE Lashuel Lab plasmids REFERENCE 2 (bases 1 to 2892) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2892 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 7..595 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 496..515 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 749..766 /label=L4440 /note="L4440 vector, forward primer" primer_bind 1001..1023 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 1088..1107 /label=T7 /note="T7 promoter, forward primer" promoter 1088..1106 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" RBS 1138..1160 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS complement(1719..2579) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2342..2361 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2580..2684) /gene="bla" /label=AmpR promoter primer_bind 2752..2770 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 61 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 121 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca 181 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 241 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 301 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 361 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 421 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 481 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 541 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 601 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 661 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac 721 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg 781 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca taggaagatc 841 ttccggaaga tcttcctatg gtgcactctc agtacaatct gctctgatgc gctacgtgac 901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 1021 aggttttcac cgtcatcacc gaaacgcgcg aggcccagcg attcgaactt ctgatagact 1081 tcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag aaataatttt 1141 gtttaacttt aagaaggaga tatacatatg gatgtattca tgaaaggact ttcaaaggcc 1201 aaggagggag ttgtggctgc tgctgagaaa accaaacagg gtgtggcaga agcagcagga 1261 aagacaaaag agggtgttct ctatgtaggc tccaaaacca aggagggagt ggtgcatggt 1321 gtggcaacag tggctgagaa gaccaaagag caagtgacaa atgttggagg agcagtggtg 1381 acgggtgtga cagcagtagc ccagaagaca gtggagggag cagggagcat tgcagcagcc 1441 actggctttg tcagaaagga ccagttgggc aagaatgaag aaggagcccc acaggaagga 1501 attctggaag atatgcctgt ggatcctgac aatgaggctt atgaaatgcc ttctgaggaa 1561 gggtatcaag actacgaacc tgaagcctaa gaaatatctt tgctcccagt ttcttgagat 1621 ctgctgacag atgttccatc caagcttatc gatgataagc tgtcaaacat gagaattaaa 1681 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 1741 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 1801 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 1861 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 1921 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 1981 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 2041 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 2101 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 2161 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 2221 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 2281 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg 2341 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 2401 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 2461 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 2521 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 2581 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 2641 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 2701 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 2761 atcacgaggc cctttcgtct tcaagaataa aaggatctag gtgaagatcc tttttgataa 2821 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 2881 aaagatcaaa gg //