LOCUS Exported 2998 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION High copy number plasmid containing Hspa1a (synonym Hsp68) MiniPromoter insert. ACCESSION . VERSION . KEYWORDS pEMS1951 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2998) AUTHORS Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM TITLE Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain; Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. JOURNAL Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 10.1534/genetics.119.301984. PUBMED 30765420 REFERENCE 2 (bases 1 to 2998) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2998 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 5..22 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 6..22 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" regulatory 1068..1077 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(1206..1222) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1206..1222) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" terminator 1270..1301 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 1302..1404 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 1405..2265 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1623..1642) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" terminator 2337..2364 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(2376..2963) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2456..2475) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg 61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa 121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc 181 cgctcgcacc acgcgtgagc tccaggaaca tccaaactga gcagccgggg tcccccccac 241 cccccacccc gccccacgcg gcaactttga gcctgtgctg ggacagagcc tctagttcct 301 aaattagtcc atgaggtcag aggcagcact gccattgtaa cgcgattgga gaggatcacg 361 tcaccggaca cgcccccagg catctccctg ggtctcctaa acttggcggg gagaagtttt 421 agcccttaag ttttagcctt taacccccat attcagaact gtgcgagttg gcgaaacccc 481 acaaatcaca acaaactgta cacaacaccg agctagaggt gatctttctt gtccattcca 541 cacaggcctt agtaatgcgt cgccatagca acagtgtcac tagtagcacc agcacttccc 601 cacaccctcc ccctcaggaa tccgtactct ccagtgaacc ccagaaacct ctggagagtt 661 ctggacaagg gcggaaccca caactccgat tactcaaggg aggcggggaa gctccaccag 721 acgcgaaact gctggaagat tcctggcccc aaggcctcct ccggctcgct gattggccca 781 gcggagagtg ggcggggccg gtgaagactc cttaaaggcg cagggcggcg agcaggtcac 841 cagacgctga cagctactca gaaccaaatc tggttccatc cagagacaag cgaagacaag 901 agaagcagag cgagcggcgc gttcccgatc ctcggccagg accagccttc cccagagcat 961 ccctgccgcg gagcgcaacc ttcccaggag catccctgcc gcggagcgca actttccccg 1021 gagcatccac gccgcggagc gcagccttcc agaagcagag cgcggcgcca tggcggcgcg 1081 ccggatcgac gagagcagcg cgactggatc agttctggac gagcgagctg tcgtccgacc 1141 cgtgatctta cggcattata cgtatgatcg gtccacgatc agctagatta tctagtcagc 1201 ttgatgtcat agctgtttcc tgaggctcaa tactgaccat ttaaatcata cctgacctcc 1261 atagcagaaa gtcaaaagcc tccgaccgga ggcttttgac ttgatcggca cgtaagaggt 1321 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga 1381 ttttcaggag ctaaggaagc taaaatgagt attcaacatt tccgtgtcgc ccttattccc 1441 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 1501 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 1561 aagatccttg agagtttacg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 1621 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 1681 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatctcacg 1741 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 1801 gccaacttac ttctggcaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 1861 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 1921 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 1981 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 2041 aaagttgcag gatcacttct gcgctcggcc ctcccggctg gctggtttat tgctgataaa 2101 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 2161 ccctcccgca tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 2221 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatgagg gcccaaatgt 2281 aatcacctgg ctcaccttcg ggtgggcctt tcttgaggac ctaaatgtaa tcacctggct 2341 caccttcggg tgggcctttc tgcgttgctg gcgtttttcc ataggctccg cccccctgac 2401 gagcatcaca aaaatcgatg ctcaagtcag aggtggcgaa acccgacagg actataaaga 2461 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 2521 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 2581 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 2641 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 2701 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 2761 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 2821 gtatttggta tctgcgctct gctgaagcca gttacctcgg aaaaagagtt ggtagctctt 2881 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 2941 cgcgcagaaa aaaaggatct caagaagatc ctttgatttt ctaccgaaga aaggccca //