LOCUS       Exported                4552 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Zebrafish codon optimized B3 recombinase that acts on blown-out 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    B3rec.zf1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Tabor KM, Smith TS, Brown M, Bergeron SA, Briggman KL, Burgess HA
  TITLE     Presynaptic Inhibition Selectively Gates Auditory Transmission to 
            the Brainstem Startle Circuit.
  JOURNAL   Curr Biol. 2018 Aug 20;28(16):2527-2535.e8. doi: 
            10.1016/j.cub.2018.06.020. Epub 2018 Aug 2.
  PUBMED    30078569
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(28..50)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     150..169
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     363..385
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     377..394
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     378..394
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2330..2346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2330..2346)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2343..2365)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2354..2370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2378..2408)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2423..2444
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2561..2578)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2732..3320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2812..2831)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3491..4351)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4114..4133
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4352..4456)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4524..4542
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
       61 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
      121 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
      181 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
      241 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      301 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      361 ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta cctcgcgaat
      421 gcatctagat ccaatccaat atgagtagtt atatggatct agtggacgac gagcctgcta
      481 ccctgtacca caagtttgtg gagtgcctga aggctggaga gaacttctgt ggagacaagc
      541 tgtctggaat catcactatg gccatcctga aggccatcaa ggctctgact gaggtgaaga
      601 agaccacctt caacaagtac aagaccacca tcaagcaggg actccagtat gatgtgggat
      661 ctagcaccat ctcttttgtg taccacctga aggactgtga tgagctgagc agaggactgt
      721 ctgatgcttt tgagccttac aagttcaaga tcaagagcaa caaggaggcc acctctttca
      781 agaccctgtt cagaggacct tcttttggat ctcagaagaa ctggagaaag aaggaggtgg
      841 acagagaggt ggacaacctg ttccacagca ctgagactga tgagagcatc ttcaagttca
      901 tcctgaacac cctggacagc attgagaccc agaccaacac tgacagacag aagactgtgc
      961 tgaccttcat cctgctgatg accttcttca actgctgcag gaacaatgac ctgatgaatg
     1021 tggaccctag caccttcaag attgtgaaga acaagtttgt gggatacctg ctccaggctg
     1081 aggtgaagca gaccaagacc agaaagagca gaaacatctt cttcttccca atcagagaga
     1141 acagatttga cctgttcctg gctctgcatg acttcttcag aacctgccag ccaaccccaa
     1201 agagcagact gtctgaccag gtgtctgagc agaagtggca gctgttcaga gacagcatgg
     1261 tgattgacta caacagattc ttcagaaagt tccctgcttc tccaatcttt gccatcaagc
     1321 atggaccaaa gtctcacctg ggaagacacc tgatgaactc tttcctgcac aagaatgagc
     1381 tggactcttg ggccaactct ctgggaaact ggtcttcttc tcagaaccag agagagtctg
     1441 gagccagact gggatacact catggaggaa gagacctgcc tcagcctctg tttggattcc
     1501 tggctggata ctgtgtgaga aatgaggagg gacacattgt gggactggga ctggagaagg
     1561 acatcaatga cctgtttgat ggaatcatgg accctctgaa tgagaaggag gacactgaaa
     1621 tctgtgagtc ttatggagag tgggccaaga ttgtgagcaa ggatgtgctg atcttcctga
     1681 agagatacca cagcaagaat gcttgcagaa gataccagaa cagcaccctg tatgccagaa
     1741 ccttcctgaa gactgagtct gtgaccctgt ctggaagcaa gggatctgag gagccttctt
     1801 ctcctgtgag aatcccaatc ctgagcatgg gaaaggcttc tccttctgag ggaagaaagc
     1861 tgagagcttc tgagcatgcc aatgatgaca atgagattga gaagattgac tctgactctt
     1921 ctcagtctga ggagatccca attgagatgt ctgactctga ggatgagacc actgctagca
     1981 acatctctgg aatctacctg gacatgagca aggccaacag caatgtggtg tactctcctc
     2041 cttctcagac tggaagagct gctggagctg gaagaaagag aggagtggga ggaagaagaa
     2101 ctgtggagag caagagaaga agagtgctgg ctccaatcaa cagaggatcc cacggattcc
     2161 ctcctgaggt ggaggagcag gatgatggaa ccctgccaat gtcttgtgct caggagtctg
     2221 gaatggacag acaccctgct gcttgtgctt ctgccagaat caatgtgtaa aaatcggatc
     2281 ccgggcccgt cgactgcaga ggcctgcatg caagcttggc gtaatcatgg tcatagctgt
     2341 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2401 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2461 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2521 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     2581 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2641 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2701 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2761 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2821 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2881 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2941 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3001 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3061 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3121 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     3181 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3241 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3301 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3361 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3421 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3481 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3541 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3601 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3661 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3721 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3781 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3841 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3901 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3961 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4021 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4081 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4141 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4201 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4261 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4321 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4381 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4441 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     4501 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ct
//
