LOCUS       Exported               13371 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  2nd generation lentiviral transfer plasmid. Expresses an engineered 
            human procollagen type I alpha 1 with SBP and mGFP between the pro 
            sequence and triple helical region.
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-SBP-mGFP-COL1A1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13371)
  AUTHORS   McCaughey J, Stevenson NL, Cross S, Stephens DJ
  TITLE     ER-to-Golgi trafficking of procollagen in the absence of large 
            carriers.
  JOURNAL   J Cell Biol. 2018 Dec 26. pii: jcb.201806035. doi: 
            10.1083/jcb.201806035.
  PUBMED    30587510
REFERENCE   2  (bases 1 to 13371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13371
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             822..935
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     regulatory      951..960
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             957..1673
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1002..1023)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1263..1282)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1610..1631
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5655..6154
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5690..5713)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6087..6106
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6175..6774
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6175..6194)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6671..6691
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6788..7376
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6841..6861)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7259..7270)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7583..8216
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8345..8361)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8345..8361)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8358..8380)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8369..8385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8393..8423)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8438..8459
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8576..8593)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8747..9335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8827..8846)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9506..10366)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10129..10148
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10367..10471)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10519..10653
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10556..10575)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10610..10629
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10868..11501
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11548..11673
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12170..12403
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12588..12632
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12895..13010
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        13068..13371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcgc tagcgctacc
      301 ggactcagat ctcgacaccg gtcgccacca tgttcagctt tgtggatctg cggctcctgc
      361 tgctgcttgc cgcaactgcg ctgctcacac atggccagga agagggacag gtggaagggc
      421 aggatgagga cattcctccc atcacttgcg tccagaatgg gctgaggtac cacgatcgcg
      481 atgtctggaa acccgaaccc tgtcggattt gcgtgtgcga taatggcaag gtgctgtgtg
      541 acgacgttat ctgcgacgaa accaagaact gtcccggagc tgaggtccca gagggcgaat
      601 gctgtccggt gtgtcccgat ggcagcgagt ctcccacaga ccaggaaacg accggagtag
      661 agggcccgaa aggggacaca ggaccacgag gacccagagg tccagccgga ccacccggtc
      721 gtgatgggat accagggcaa ccagggctgc ctggccctcc tggcccacct ggacctcctg
      781 gcccgcccgg cttgggtggc aactttgcag ccgctgcagc gatggacgaa aagaccaccg
      841 gatggagagg tgggcacgtt gtggaaggtc ttgctggcga gttggagcag ctgcgcgcca
      901 gactcgagca tcacccacaa gggcagagag agcccatgaa gacaccggtc gccaccatgg
      961 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1021 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1081 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1141 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     1201 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1261 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1321 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1381 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     1441 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1501 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1561 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1621 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtgctgca
     1681 gctgccccca gctgtcttat ggctatgatg agaaatcaac cggaggaatt tccgtgcctg
     1741 gccccatggg tccctctggt cctcgtggtc tccctggccc ccctggtgca cctggtcccc
     1801 aaggcttcca aggtccccct ggtgagcctg gcgagcctgg agcttcaggt cccatgggtc
     1861 cccgaggtcc cccaggtccc cctggaaaga atggagatga tggggaagct ggaaaacctg
     1921 gtcgtcctgg tgagcgtggg cctcctgggc ctcagggtgc tcgaggattg cccggaacag
     1981 ctggcctccc tggaatgaag ggacacagag gtttcagtgg tttggatggt gccaagggag
     2041 atgctggtcc tgctggtcct aagggtgagc ctggcagccc tggtgaaaat ggagctcctg
     2101 gtcagatggg cccccgtggc ctgcctggtg agagaggtcg ccctggagcc cctggccctg
     2161 ctggtgctcg tggaaatgat ggtgctactg gtgctgccgg gccccctggt cccaccggcc
     2221 ccgctggtcc tcctggcttc cctggtgctg ttggtgctaa gggtgaagct ggtccccaag
     2281 ggccccgagg ctctgaaggt ccccagggtg tgcgtggtga gcctggcccc cctggccctg
     2341 ctggtgctgc tggccctgct ggaaaccctg gtgctgatgg acagcctggt gctaaaggtg
     2401 ccaatggtgc tcctggtatt gctggtgctc ctggcttccc tggtgcccga ggcccctctg
     2461 gaccccaggg ccccggcggc cctcctggtc ccaagggtaa cagcggtgaa cctggtgctc
     2521 ctggcagcaa aggagacact ggtgctaagg gagagcctgg ccctgttggt gttcaaggac
     2581 cccctggccc tgctggagag gaaggaaagc gaggagctcg aggtgaaccc ggacccactg
     2641 gcctgcccgg accccctggc gagcgtggtg gacctggtag ccgtggtttc cctggcgcag
     2701 atggtgttgc tggtcccaag ggtcccgctg gtgaacgtgg ttctcctggc cctgctggcc
     2761 ccaaaggatc tcctggtgaa gctggtcgtc ccggtgaagc tggtctgcct ggtgccaagg
     2821 gtctgactgg aagccctggc agccctggtc ctgatggcaa aactggcccc cctggtcccg
     2881 ccggtcaaga tggtcgcccc ggacccccag gcccacctgg tgcccgtggt caggctggtg
     2941 tgatgggatt ccctggacct aaaggtgctg ctggagagcc cggcaaggct ggagagcgag
     3001 gtgttcccgg accccctggc gctgtcggtc ctgctggcaa agatggagag gctggagctc
     3061 agggaccccc tggccctgct ggtcccgctg gcgagagagg tgaacaaggc cctgctggct
     3121 cccccggatt ccagggtctc cctggtcctg ctggtcctcc aggtgaagca ggcaaacctg
     3181 gtgaacaggg tgttcctgga gaccttggcg cccctggccc ctctggagca agaggcgaga
     3241 gaggtttccc tggcgagcgt ggtgtgcaag gtccccctgg tcctgctggt ccccgagggg
     3301 ccaacggtgc tcccggcaac gatggtgcta agggtgatgc tggtgcccct ggagctcccg
     3361 gtagccaggg cgcccctggc cttcagggaa tgcctggtga acgtggtgca gctggtcttc
     3421 cagggcctaa gggtgacaga ggtgatgctg gtcccaaagg tgctgatggc tctcctggca
     3481 aagatggcgt ccgtggtctg accggcccca ttggtcctcc tggccctgct ggtgcccctg
     3541 gtgacaaggg tgaaagtggt cccagcggcc ctgctggtcc cactggagct cgtggtgccc
     3601 ccggagaccg tggtgagcct ggtccccccg gccctgctgg ctttgctggc ccccctggtg
     3661 ctgacggcca acctggtgct aaaggcgaac ctggtgatgc tggtgctaaa ggcgatgctg
     3721 gtccccctgg ccctgccgga cccgctggac cccctggccc cattggtaat gttggtgctc
     3781 ctggagccaa aggtgctcgc ggcagcgctg gtccccctgg tgctactggt ttccctggtg
     3841 ctgctggccg agtcggtcct cctggcccct ctggaaatgc tggaccccct ggccctcctg
     3901 gtcctgctgg caaagaaggc ggcaaaggtc cccgtggtga gactggccct gctggacgtc
     3961 ctggtgaagt tggtccccct ggtccccctg gccctgctgg cgagaaagga tcccctggtg
     4021 ctgatggtcc tgctggtgct cctggtactc ccgggcctca aggtattgct ggacagcgtg
     4081 gtgtggtcgg cctgcctggt cagagaggag agagaggctt ccctggtctt cctggcccct
     4141 ctggtgaacc tggcaaacaa ggtccctctg gagcaagtgg tgaacgtggt ccccctggtc
     4201 ccatgggccc ccctggattg gctggacccc ctggtgaatc tggacgtgag ggggctcctg
     4261 gtgccgaagg ttcccctgga cgagacggtt ctcctggcgc caagggtgac cgtggtgaga
     4321 ccggccccgc tggaccccct ggtgctcctg gtgctcctgg tgcccctggc cccgttggcc
     4381 ctgctggcaa gagtggtgat cgtggtgaga ctggtcctgc tggtcccgcc ggtcctgtcg
     4441 gccctgttgg cgcccgtggc cccgccggac cccaaggccc ccgtggtgac aagggtgaga
     4501 caggcgaaca gggcgacaga ggcataaagg gtcaccgtgg cttctctggc ctccagggtc
     4561 cccctggccc tcctggctct cctggtgaac aaggtccctc tggagcctct ggtcctgctg
     4621 gtccccgagg tccccctggc tctgctggtg ctcctggcaa agatggactc aacggtctcc
     4681 ctggccccat tgggccccct ggtcctcgcg gtcgcactgg tgatgctggt cctgttggtc
     4741 cccccggccc tcctggacct cctggtcccc ctggtcctcc cagcgctggt ttcgacttca
     4801 gcttcctgcc ccagccacct caagagaagg ctcacgatgg tggccgctac taccgggctg
     4861 atgatgccaa tgtggttcgt gaccgtgacc tcgaggtgga caccaccctc aagagcctga
     4921 gccagcagat cgagaacatc cggagcccag agggcagccg caagaacccc gcccgcacct
     4981 gccgtgacct caagatgtgc cactctgact ggaagagtgg agagtactgg attgacccca
     5041 accaaggctg caacctggat gccatcaaag tcttctgcaa catggagact ggtgagacct
     5101 gcgtgtaccc cactcagccc agtgtggccc agaagaactg gtacatcagc aagaacccca
     5161 aggacaagag gcatgtctgg ttcggcgaga gcatgaccga tggattccag ttcgagtatg
     5221 gcggccaggg ctccgaccct gccgatgtgg ccatccagct gaccttcctg cgcctgatgt
     5281 ccaccgaggc ctcccagaac atcacctacc actgcaagaa cagcgtggcc tacatggacc
     5341 agcagactgg caacctcaag aaggccctgc tcctccaggg ctccaacgag atcgagatcc
     5401 gcgccgaggg caacagccgc ttcacctaca gcgtcactgt cgatggctgc acgagtcaca
     5461 ccggagcctg gggcaagaca gtgattgaat acaaaaccac caagtcctcc cgcctgccca
     5521 tcatcgatgt ggcccccttg gacgttggtg ccccagacca ggaattcggc ttcgacgttg
     5581 gccctgtctg cttcctgtaa ctgcagtcga cggtaccgcg ggcccgggat cccgcgactc
     5641 tagataattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     5701 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     5761 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     5821 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     5881 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     5941 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     6001 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     6061 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     6121 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc
     6181 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     6241 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6301 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6361 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6421 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6481 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6541 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6601 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6661 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6721 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt
     6781 ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6841 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6901 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6961 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7021 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7081 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7141 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     7201 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7261 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7321 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     7381 aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     7441 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     7501 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7561 tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc
     7621 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
     7681 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     7741 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
     7801 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
     7861 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
     7921 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
     7981 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
     8041 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8101 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8161 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     8221 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     8281 tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat
     8341 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8401 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8461 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8521 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8581 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8641 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8701 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8761 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8821 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8881 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8941 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9001 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9061 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9121 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9181 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9241 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9301 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9361 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9421 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9481 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9541 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9601 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9661 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9721 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9781 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9841 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9901 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9961 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10021 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10081 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10141 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10201 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10261 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10321 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10381 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10441 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10501 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    10561 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    10621 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
    10681 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
    10741 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
    10801 tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt actacaaact
    10861 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
    10921 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
    10981 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
    11041 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
    11101 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
    11161 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
    11221 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
    11281 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
    11341 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    11401 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    11461 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
    11521 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
    11581 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
    11641 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
    11701 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
    11761 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
    11821 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    11881 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    11941 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    12001 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
    12061 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
    12121 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
    12181 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
    12241 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
    12301 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
    12361 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
    12421 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
    12481 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
    12541 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
    12601 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
    12661 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
    12721 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
    12781 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
    12841 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgcctttaaa
    12901 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    12961 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    13021 tacagggaca gcagagatcc agtttatcga taagcttggg agttccgcgt tacataactt
    13081 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
    13141 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
    13201 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
    13261 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
    13321 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat g
//
