LOCUS Exported 1248 bp ds-DNA linear SYN 15-JUN-2021 DEFINITION Bacterial expression of proISG15. ACCESSION . VERSION . KEYWORDS ISG15 C-term. Domain deltaC intein/chitin binding domain (79-154) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1248) AUTHORS Swatek KN, Aumayr M, Pruneda JN, Visser LJ, Berryman S, Kueck AF, Geurink PP, Ovaa H, van Kuppeveld FJM, Tuthill TJ, Skern T, Komander D TITLE Irreversible inactivation of ISG15 by a viral leader protease enables alternative infection detection strategies. JOURNAL Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2371-2376. doi: 10.1073/pnas.1710617115. Epub 2018 Feb 20. PUBMED 29463763 REFERENCE 2 (bases 1 to 1248) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 15, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1248 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 329..922 /codon_start=1 /gene="Mycobacterium xenopi gyrA" /product="modified GyrA intein (Southworth et al., 1999)" /label=Mxe GyrA intein /note="N-terminal cleavage of this intein can be induced by thiols." /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA" CDS 953..1105 /codon_start=1 /gene="Bacillus circulans chiA" /product="chitin binding domain from chitinase A1 (Watanabe et al., 1994)" /label=CBD /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV PALWQL" ORIGIN 1 gtttaacttt aagaaggaga tatacatatg gctagctcgc gagtcgacgg cggccgcgaa 61 ttcctcgagg gctcttcctg cggaagagca accacctatg gatgaaccgc tgagcattct 121 ggtgcgtaat aacaaaggtc gtagcagcac ctatgaagtt cgtctgaccc agacagttgc 181 acatctgaaa cagcaggtta gcggtctgga aggtgttcag gatgacctgt tttggctgac 241 ctttgaaggt aaaccgctgg aagatcagct gccgctgggt gaatatggtc tgaaaccgct 301 gtcaaccgtt tttatgaatc tgcgtctgtg catcacggga gatgcactag ttgccctacc 361 cgagggcgag tcggtacgca tcgccgacat cgtgccgggt gcgcggccca acagtgacaa 421 cgccatcgac ctgaaagtcc ttgaccggca tggcaatccc gtgctcgccg accggctgtt 481 ccactccggc gagcatccgg tgtacacggt gcgtacggtc gaaggtctgc gtgtgacggg 541 caccgcgaac cacccgttgt tgtgtttggt cgacgtcgcc ggggtgccga ccctgctgtg 601 gaagctgatc gacgaaatca agccgggcga ttacgcggtg attcaacgca gcgcattcag 661 cgtcgactgt gcaggttttg cccgcgggaa acccgaattt gcgcccacaa cctacacagt 721 cggcgtccct ggactggtgc gtttcttgga agcacaccac cgagacccgg acgcccaagc 781 tatcgccgac gagctgaccg acgggcggtt ctactacgcg aaagtcgcca gtgtcaccga 841 cgccggcgtg cagccggtgt atagccttcg tgtcgacacg gcagaccacg cgtttatcac 901 gaacgggttc gtcagccacg ctactggcct caccggtctg aactcaggcc tcacgacaaa 961 tcctggtgta tccgcttggc aggtcaacac agcttatact gcgggacaat tggtcacata 1021 taacggcaag acgtataaat gtttgcagcc ccacacctcc ttggcaggat gggaaccatc 1081 caacgttcct gccttgtggc agcttcatga ctgcnnggaa ggggatccgg ctgctaacaa 1141 agcccgaaag gaagctgaat tggctgctgc caccgctgaa caataactag cataacccct 1201 tggggcctct aaacggggtc ttggagggtt ttttgctgaa aggaggaa //