LOCUS Exported 3492 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION In situ hybridization and RNAi studies in planarians. ACCESSION . VERSION . KEYWORDS Ddo_innexin-10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3492) AUTHORS Accorsi A, Williams MM, Ross EJ, Robb SMC, Elliott SA, Tu KC, Sanchez Alvarado A TITLE Hands-On Classroom Activities for Exploring Regeneration and Stem Cell Biology with Planarians JOURNAL The American Biology Teacher 79(3):208-223. Mar 2017. REFERENCE 2 (bases 1 to 3492) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3492 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 1..17 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" terminator 24..71 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" promoter 76..94 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 152..168 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 152..168 /label=pBluescriptSK /note="For pBluescript vector" promoter complement(1122..1140) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter complement(1198..1216) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1221..1237) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1221..1237) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1339..1358) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1350..2159 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1417..1436) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2306..2894 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2795..2814 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3048..3065 /label=L4440 /note="L4440 vector, forward primer" terminator 3224..3251 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3343..3429 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 3478..8 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3492..17 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" ORIGIN 1 gtaaaacgac ggccagtctt aagcaaaaaa cccctcaaga cccgtttaga ggccccaagg 61 ggttatgcta ggaagtaata cgactcacta tagggagacc ggcagatctg atatcatcga 121 tgaattcgag ctccaccgcg gtggcggccg ctctagaact agtggatcca ccggttccat 181 ggctagcggc attaccatcc cgggaacaat ctcgatcata gctatggttc tgtcagagtt 241 cattgattac gctaccaaat acaaatgggt ggattttgtg ggcatggaag actttggcga 301 ccggttaagt ttcaaccttt ccacttcgat attattgctt ctcactgttg ttatatcagt 361 taaaagttat ttgttagctt caattgcttg ttatattcct attcaaccaa ccggtcatga 421 tttcgatgat tttttgaaga gttattgttg ggtacatgga accataccga tgcataataa 481 tcgtcccatg ccacgaactg aaaatgaatg ggaatatgca gacaaaatgg aaagaataaa 541 ttattatcaa tgggttcctt ttgtacttgc tttacaaggg gtcctttttt atttaccgag 601 aatcatatgg caaatgatca cttacaactt atctgggaca gatctggaag gactcgtgga 661 aatggcgaat gctgctagta atcaaactgg tcaagcgaaa gcagaaactg taaatcaagt 721 cgcaatgaat ctggaaaatc tgttgttttc ccatagaaaa attcgaaatc atagaaaagt 781 aataatgttg aaactttctg aagctaaatc ttggttttgt gctggaaaaa gaagtggtcg 841 gcaattaatc gttcaatata tgttcattaa attcttatat ttaattaatg ctttcggaca 901 acttatactc atgcaatact tcttgaattt cggtcacaat gactcgagct ttggttacac 961 ggttcttaag aacatactaa acgggaaaga atggaatgat actttaatat ttccaagggt 1021 cacatattgt tatgtaaaaa atattaaaca tgttggaata acaaatgatt acttagctca 1081 atgcgccggg tagaattggc cgggcccggt acccaattcg ccctatagtg agtcgtatta 1141 cttccacata accccttggg gcctctaaac gggtcttgag gggttttttg aggatcccct 1201 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa 1261 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg 1321 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc 1381 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg 1441 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt 1501 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa 1561 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg 1621 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat 1681 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt 1741 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc 1801 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct 1861 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag 1921 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag 1981 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat 2041 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta 2101 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat 2161 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg 2221 caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc 2281 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 2341 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 2401 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 2461 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 2521 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 2581 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 2641 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 2701 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 2761 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 2821 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 2881 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 2941 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 3001 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 3061 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 3121 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 3181 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag 3241 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc 3301 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag 3361 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg 3421 ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc 3481 agtcacgacg tt //