LOCUS Exported 11053 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags. . ACCESSION . VERSION . KEYWORDS PF3D7_1462300-COMP-blac-flag-his SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11053) TITLE Pentamerised full-length protein ectodomains with no N-linked glycans REFERENCE 2 (bases 1 to 11053) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11053 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 310..329 /label=pMT2-F /note="Synthetic intron, forward primer" regulatory 352..361 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4585..5370 /codon_start=1 /gene="bla(M)" /product="beta-lactamase lacking the signal sequence" /label=bla(M) /note="allows cytosolic expression of beta-lactamase" /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW" CDS 5377..5442 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 5449..5466 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" polyA_signal 5504..5559 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(5558..5577) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(7795..7813) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7881..7985 /gene="bla" /label=AmpR promoter CDS 7986..8846 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 9017..9605 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9506..9525 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9759..9776 /label=L4440 /note="L4440 vector, forward primer" protein_bind 9893..9914 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9929..9959 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9967..9983 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9972..9994 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9991..10007 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9991..10007 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" enhancer 10278..10657 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 10658..10861 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 10812..10832 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 10858..10882 /label=LNCX /note="Human CMV promoter, forward primer" ORIGIN 1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg 61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta 121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt 181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca 241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg 301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg 361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccatc 421 tactactgca acgacaacat cctggaaaac gccaacaacg acgacagcac caaggacatc 481 aacaatatca acaagaacag cgccaacaag gccaagagcg agaaaaccag caactacaac 541 aacgatcagt acaacatcga cgacgcctac aacgaggacg aggaaaacga cgaggaagag 601 ggcgacgacg acgagaacga ccacttcgag gtgcccatca actgcgctgg cggcaactgc 661 ttcgacagca tggacgacat taacaagatc atcgacaaca agaaaaagaa gatgaagtac 721 cggcggaggc ggggcaaggc catccagatc atcaagccca acgtgaacca cgccgagctg 781 atcatcatcg aagagaacct ggaaatcttc aagcggatcg acaagcctat cgccatcgtg 841 tccgtgctgg gcgacatgca caccggcaag agctttctgc tgaacctgct gaatgagcac 901 gtgatccccg atattaacaa agccaacaag tacatcgaga acggcttcaa agtgggcaac 961 gacatcaccg ccagcaccta cggcatctgg atttggagcg agcccatccg gatcaacgtg 1021 aagaagctga agaagatcta cgagtacatc aacgagaact tcgccaactt catcaagagc 1081 tatgagtacg agaaggacga gtacaatgag gaaatcatcg acctgctcaa cctgtacaag 1141 accgacgact ggatcagcaa cgtcgaggaa ctgaacctgg ccgacaccga cgaagtgaac 1201 ctgatcctga tggacaccca gggcctgaac agccccaatg tgaacaagag atacgacgag 1261 atcctgtacg ccctgaccaa cctgatcagc accgacatca tctttctgac catgaagatg 1321 attaacaaca aggacctcga gttcatcgag aatatcgcca aggacgccaa cctgttcatg 1381 ctgcgggcct acaccagaag cagcggcagc accttcagca tcaagaagaa caacttccac 1441 aagatcctgg acaccctcaa caacaacctc gagaacaacg ccctgattct ggaatctatc 1501 gccagcaaga acctgatgtg ggtggtgcac gacttcagcc agcggctgga cgtgcggaag 1561 ggcaagctgt ggctggacat cctgctgaac agcgaccggc gggacctgga catcaagtac 1621 tggaagaaga ttatcagcaa caaggctaag gacaagcacg acgcttacac caccaagaag 1681 cagaagatcg cctacctgac ctacaatgag aactccgcca gcggcctgct gcccaacaac 1741 aataataaca ataacaatgc caacgacgcc tcctccaact acaagctgaa catcctgtat 1801 aagaacatcg actgcgtgct gctgcggaac atgtaccaca acaaagagtt cgacttcacc 1861 aaggccaaca tcaatgacct gaacgaagag tacaagaacg atgtgctgct cctgcggtac 1921 aagatctacc tgcgggccct gctgttcccc aagaaaatgt acagcgacgt gcagatcaac 1981 aacatcatca acaaaatccg gaaagagcag aagctgcgcg aggaacagga aaagaacaag 2041 gacaacaaaa agaacgacgc taacaaccag gccaacgacc tgaactccaa caaggatgag 2101 acaagcggca gccaggtcac aaaccggttc atgagcggcc acgacgtgta cgacttcatc 2161 acctttctgg tcaagagcgc caaccagaat ctgttcacca acgtcaacca gttcttcaag 2221 cacttcaaga tcaaccgggc cgagatcagc cggaacgatc tgatcttcct gtacaaaaag 2281 tacctgctgc agttcatgga aaacgaggat gtggaattca tcagcaacct gaatgatgag 2341 aacgacgacc tgttcctgca gaaagagggc aacaacgccg ccgtgaatca cgatgagaat 2401 agcgacgccg acagcctgtt caacgaccac gacgaaaaca tgcagctgaa agagcacaac 2461 atcaaggacg gcgaggacaa cacccaccag aagaaggaca gctataagct gcctccactg 2521 ctgaacgaga tcaagaagta cgaagaactg atccgcgagc agatcctgaa catctggtac 2581 aagtacacag agagcgactt tcacgacgaa gaggaaaaag agctgattaa ggacattgag 2641 aacaacctct acgagcgcct ggaccagatc aagatcgaga tgctggaact gggcgagatg 2701 aacatccgga agttctgcaa gattgcctgc gaggacgccc tgcagatcgt ggtggaagat 2761 atcaaaatga agtccgacca gtaccccatc aagcagaagg acctgaccac cttcttcgag 2821 agcgtgtcct acaatctgat caagtacctc gaaaagaagc tgtccaagaa ctccgacaag 2881 ctggacctga tctactacaa ggataacatc tgcagccctc tgatcaacaa tgccttccag 2941 gaattctact tcctgaagaa aaaaaacatt gccctgaatg agaaccgcct gaagtcctac 3001 ttcagcaacg ccgtggaaaa gggcaaagag gtgttcgaag tcctggccga gaacaccgag 3061 aatattgccg agtacttcaa gaacaagaat atgttctaca ccgtgctgga caagtggacc 3121 agcgaggcca tcaacaccta caccctgacc ctgagcgatt tcgccaaaga agagaaagag 3181 atcaacgacg agtacctgaa tgtgctgaac aacgatatca agctgctgaa acagaaagcc 3241 atggaaaagt ggcacaacca ctgccgggac aagacctccg ccctgtacaa catgcacaag 3301 tccaacctga aaaagaactt cctcgagaat ttcaacttcc cactggacga gctggtgctg 3361 caggacatct tcctgaacct gaagggccag gaagaactca agtacatgga catctactgc 3421 gccaacgagg atagctggaa cagcgagtac aaaaacttcc tcaagctgac cgatgagatc 3481 ttccagttca ttaaggacga gaacctcaaa gccattcaga tcacctgtca gcagcccctg 3541 gacgaactga agaacggcat caagggcgag atccacaagt actacctgtg gtcctccctg 3601 aagtctaccc tgtataaccg ggccctgatc gtgctgagca gcaatatcga gaacatctac 3661 atcaagaacc agcgcgagaa caagatccag agcaacctgg acgagaagta caacaacaaa 3721 aacaagaact acaagcggat ctccaaggac ctgatgagca aagtgatcaa ccggtggctc 3781 aacaacgaca tctacaacgt gtactacccc ggcgcgccgt cgacctccat cacggcctat 3841 aagagtgagg gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg 3901 cagggagagt tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc 3961 tccctaaaga accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg 4021 tccgaaacgc tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct 4081 ggcaacctga ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg 4141 atgaaagtga ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca 4201 cccaagatga gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag 4261 aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt 4321 gaagaggtca agatggactc caagatccag gttttatcca aagggttgaa ttccggcggc 4381 ggctccggag gcggtaccgg tggcggcgac ctagccccac agatgcttcg agaactccag 4441 gagactaatg cggcgctgca agacgtgaga gagctcttgc gacagcaggt caaggagatc 4501 accttcctga agaatacggt gatggaatgt gacgcctgcg gtctagacag aaacctgcct 4561 ccactagcac ctctgggccc acacccagaa acgctggtga aagtaaaaga tgctgaagat 4621 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 4681 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 4741 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 4801 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 4861 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 4921 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 4981 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 5041 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 5101 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 5161 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 5221 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 5281 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 5341 gagataggtg cctcactgat taagcattgg ggccctgact acaaagacca tgacggtgat 5401 tataaagatc atgacatcga ttacaaggat gacgatgaca agggccctca ccaccaccat 5461 caccactaat taattaagga tcccccgacc tcgacctctg gctaataaag gaaatttatt 5521 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg 5581 gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat ccccgccccg 5641 gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt gcatgtaatc 5701 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg 5761 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat 5821 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac 5881 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt 5941 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg 6001 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc 6061 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta 6121 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag 6181 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta 6241 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa 6301 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt 6361 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa 6421 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg 6481 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat 6541 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac 6601 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg 6661 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta 6721 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt 6781 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga 6841 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc 6901 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc 6961 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct 7021 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt 7081 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg 7141 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata 7201 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta 7261 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta 7321 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta 7381 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta 7441 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat 7501 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat 7561 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat 7621 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat 7681 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat 7741 atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga aagggcctcg 7801 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 7861 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 7921 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 7981 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 8041 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 8101 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 8161 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 8221 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 8281 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 8341 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 8401 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 8461 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 8521 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 8581 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 8641 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 8701 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 8761 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 8821 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 8881 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 8941 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 9001 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 9061 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 9121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 9181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 9241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 9301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 9361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 9421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 9481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 9541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 9601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 9661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 9721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 9781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 9841 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 9901 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 9961 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 10021 agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc agatccgggc 10081 aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct atacattgaa 10141 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta 10201 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 10261 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg 10321 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 10381 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 10441 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 10501 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 10561 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 10621 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 10681 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 10741 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 10801 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 10861 tcgtttagtg aaccgtcaga tcctcactct cttccgcatc gctgtctgcg agggccagct 10921 gttgggctcg cggttgagga caaactcttc gcggtctttc cagtactctt ggatcggaaa 10981 cccgtcggcc tccgaacggt actccgccac cgagggacct gagcgagtcc gcatcgaccg 11041 gatcggaaaa cct //