LOCUS Exported 4020 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION In situ hybridization and RNAi studies in planarians. ACCESSION . VERSION . KEYWORDS Ddo_IFB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4020) AUTHORS Accorsi A, Williams MM, Ross EJ, Robb SMC, Elliott SA, Tu KC, Sanchez Alvarado A TITLE Hands-On Classroom Activities for Exploring Regeneration and Stem Cell Biology with Planarians JOURNAL The American Biology Teacher 79(3):208-223. Mar 2017. REFERENCE 2 (bases 1 to 4020) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4020 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(25..42) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(196..784) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(276..295) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(931..1740) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 1654..1673 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 1732..1751 /label=pENTR-R /note="pENTR vectors, reverse primer" primer_bind 1853..1869 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 1853..1869 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 1874..1892 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 1950..1968 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3450..3466) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3450..3466) /label=pBluescriptSK /note="For pBluescript vector" promoter complement(3524..3542) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" terminator 3547..3594 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(3601..3618) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3601..3617) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3610..3632) /label=M13/pUC Forward /note="In lacZ gene" terminator 3681..3767 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 3859..3886 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" ORIGIN 1 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 61 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact 841 cacgttaagg gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg 901 ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa 961 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa 1021 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc 1081 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt 1141 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg 1201 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc 1261 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct 1321 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc 1381 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc 1441 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt 1501 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt 1561 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa 1621 gcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa 1681 atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat 1741 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt 1801 tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccagagct gccaggaaac 1861 agctatgacc atgtaatacg actcactata ggggatcctc aaaaaacccc tcaagacccg 1921 tttagaggcc ccaaggggtt atgtggaagt aatacgactc actatagggc gaattgggta 1981 ccgggcccgg ccaattctac ccgagcttca cttgatcctt gaggtttttg acccttcgcc 2041 caaattttat agtatctgtt ttttcctgtt ggttctaata tcgttttcgc tggaatcaca 2101 tattttataa cgattcttcc attatcagta tttctattta atacgtatcc agatacatct 2161 tcttgctgtg aacctgtatt ctcaaggatt ataaattttc catctggaga acattcttgt 2221 attgtcacat ttccttttgc agtcctctga aacgaagttt ttgcagacat ttctcctttc 2281 acaacatttg taatctgtgt tttcgtcatt ggggatgtat gttgctgact aatcgtagat 2341 ctggtcatat ggtcagtcaa atccatacta tgacctcctt gactgaattg agcatcttta 2401 cggttcactc tgtattcttc tccttcaagc atcttcctat aggctgctat ttcaagttcc 2461 aaacttaatt tggcatcgag taaaagttgt aattccaaaa caatattttc taattctgct 2521 tgacgattca tcaattcctt tcttaaatga atcttctctg attcaaactc tctatgcttt 2581 tcgtctattt ctcgtttaag catatctagc attcgttcaa gatcgttgtt tcttaactct 2641 aattcaggta acctatttct catttctacc aattgttcct ttaatctcgc attttcttcc 2701 cttaactgaa ctgtttcagc gctttctttt gtttgggtcg tacggaattc ttgaatttta 2761 agagtgtatt gagcttccat ttccatttga agagtttcca tttttctatc atattcattt 2821 tgaatatctc gtaaagcttg agccatttcg tttctccaga agtctttgct atccgaagca 2881 tctttatata tcaatgatga taattctcgt atttctgatt caaagatttt cttcaaaaac 2941 tcaatctgat ctagaagaga ttgaacttca ttttgagcag taatatgttc aagtgtttct 3001 ttatcaagat ccttcttcag ttggtttatt tcttctttca gtctaaaaat ttctttattg 3061 tctcgaactt tttcttcttc taatccagcc aatcttctgc gaagactcga aatttcgtgt 3121 tcgtaatcgg aaagttgatt tgtcatttta tctattgttt ccttatcgga aatgtggagt 3181 tgattcacat catttaattc tatttgagct tcttgaatca aatcctctat tgaattaatt 3241 cgcatttcta attgcgcttt tgaagcttca gcttgatcca aagactttct gagtaacacc 3301 atgtcattat catacagtgc tttgattctt gctgtctctt ttccccattg gtcttttaaa 3361 tccattaact cctctgatag tcgtttattt tcatcctcca gatgtcgcac tttatcggga 3421 tggtaatgcc gctagccatg gaaccggtgg atccactagt tctagagcgg ccgccaccgc 3481 ggtggagctc gaattcatcg atgatatcag atctgccggt ctccctatag tgagtcgtat 3541 tacttcctag cataacccct tggggcctct aaacgggtct tgaggggttt tttgcttaag 3601 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag 3661 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc 3721 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg 3781 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg 3841 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa 3901 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc 3961 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt //