LOCUS Exported 3696 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION In situ hybridization and RNAi studies in planarians. ACCESSION . VERSION . KEYWORDS Pmo_PC2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3696) AUTHORS Accorsi A, Williams MM, Ross EJ, Robb SMC, Elliott SA, Tu KC, Sanchez Alvarado A TITLE Hands-On Classroom Activities for Exploring Regeneration and Stem Cell Biology with Planarians JOURNAL The American Biology Teacher 79(3):208-223. Mar 2017. REFERENCE 2 (bases 1 to 3696) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3696 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(35..52) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(206..794) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(286..305) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(941..1750) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 1664..1683 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 1742..1761 /label=pENTR-R /note="pENTR vectors, reverse primer" primer_bind 1863..1879 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 1863..1879 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 1884..1902 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 1960..1978 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3136..3152) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3136..3152) /label=pBluescriptSK /note="For pBluescript vector" promoter complement(3210..3228) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" terminator 3233..3280 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(3287..3304) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3287..3303) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3296..3318) /label=M13/pUC Forward /note="In lacZ gene" terminator 3367..3453 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 3545..3572 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" ORIGIN 1 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 61 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 841 acgcgtaact cacgttaagg gattttggtc atgagcttgc gccgtcccgt caagtcagcg 901 taatgctctg ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca 961 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc 1021 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt 1081 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa 1141 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca 1201 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa 1261 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata 1321 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca 1381 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg 1441 ctgtttttcc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat 1501 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg 1561 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct 1621 tcccatacaa gcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat 1681 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa 1741 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg 1801 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccagagct 1861 gccaggaaac agctatgacc atgtaatacg actcactata ggggatcctc aaaaaacccc 1921 tcaagacccg tttagaggcc ccaaggggtt atgtggaagt aatacgactc actatagggc 1981 gaattgggta ccgggcccgg ccaattctac ccgatcgtat ggtcgatctt gagtgccatg 2041 aagaacgagg acccactcag agagcagtcc ttggatcagg gcgtcctctg tatcgaacat 2101 gacggttagt cgccacttgc cgcgagggtt ctctccccag gtgtgcgtgg tcatgaaggg 2161 ccatttggta aacccgcgga tcgagtcact gtctttctct cgacgactaa ggatcatcga 2221 cgtcgttccc attggactcg tcagaaacat cgttatgtca cctcgacgac ttgctttcac 2281 agtcacaaat gcttgtacgt gttctaagta gttcactctg tggttatcat cggatttgca 2341 agcgtccgtg tcaatctcca gcacaagtgc tttgctgata ttaaacctcg atttttggtt 2401 gatctctcca gcaatgcaat gaaacctttc tggaacgttg gcccacatct tcgccactcg 2461 gaccatgtca cctgcgtcta gcaccccata gccgaataag tgattgaact cgagacctgc 2521 accattaatc gtccagttat gtcgcccgtc cctgtcgtgc aaatggttcc tcttgctagt 2581 cagtactgac aaatgctgca tgtctctcca gcccatttcc ccgttcgctt caagggcaag 2641 agcaaagacc gctgctgcct ctggagcggc ggcggatgtg ccagaatggt ggagtgtaca 2701 ttggccgtac agatccactg tgccgactcc ggcatccaga ctgaaccaag ccttgccatt 2761 ggagaatgtg gaagcgaggg tcgaagaaca cgactcatca taactcgctg tttcgccgtc 2821 attcgtagcg gaattgatgc tgatcgtcca catgctcgcc gcgtatccat cgcaattgca 2881 atcgtctgag gcaccaccgt ctcctgaagc ccagacataa atgttacctt tgccgttgcg 2941 gcccttattg accccgtcaa caatggccct catggtttcg ttgcggggtc catcgacagt 3001 tctaccgtca tcggtggggc cccaactggc ggagtaaata tggatattgt cgcgatcatg 3061 gctcatcgct gcagcttcaa tgagatccgt catgtatggt tcgggatggt aatgccgcta 3121 gccatggaac cggtggatcc actagttcta gagcggccgc caccgcggtg gagctcgaat 3181 tcatcgatga tatcagatct gccggtctcc ctatagtgag tcgtattact tcctagcata 3241 accccttggg gcctctaaac gggtcttgag gggttttttg cttaagactg gccgtcgttt 3301 tacaacgtcg tgactgggaa aacatccatg ctagcgttaa cgcgagagta gggaactgcc 3361 aggcatcaaa taaaacgaaa ggctcagtcg gaagactggg cctttcgttt tatctgttgt 3421 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgtg 3481 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaact 3541 aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct tcctggctag 3601 cggtacgcgt attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 3661 gccagctgca ttaatgaatc ggccaacgcg cgggga //