LOCUS Exported 4811 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Retroviral vector for cloning and expressing your gene of interest. Zeocin selection.. ACCESSION . VERSION . KEYWORDS pBABE-zeo (pBABE-bleo) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4811) AUTHORS Morgenstern JP, Land H TITLE Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. JOURNAL Nucleic Acids Res. 1990 Jun 25;18(12):3587-96. PUBMED 2194165 REFERENCE 2 (bases 1 to 4811) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4811 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 9..478 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 543..742 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 943..1359 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1289..1311 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1327..1343 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" primer_bind complement(1417..1437) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 1422..1751 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 1602..1737 /label=SV40 ori /note="SV40 origin of replication" primer_bind 1664..1683 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 1761..2135 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" LTR 2306..2775 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(2905..2922) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3076..3664) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3156..3175) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3824..4684) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4447..4466 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4685..4789) /gene="bla" /label=AmpR promoter ORIGIN 1 cctgcagcct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc 61 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag 121 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca 181 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc 241 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg 301 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt 361 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg 421 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt 481 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag 541 gcaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt 601 atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga 661 actgacgagt tctgaacacc cggccgcaac cctgggagac gtcccaggga ctttgggggc 721 cgtttttgtg gcccgacctg aggaagggag tcgatgtgga atccgacccc gtcaggatat 781 gtggttctgg taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt 841 tcggtttgga accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt 901 tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact 961 cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg 1021 gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac 1081 gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag 1141 gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa 1201 gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct 1261 cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga 1321 tcctcccttt atccagccct cactccttct ctaggcgccg gccggatccc agtgtggtgg 1381 tacgtaggaa ttcgccagca cagtggtcga ccctgtggaa tgtgtgtcag ttagggtgtg 1441 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 1501 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 1561 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 1621 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 1681 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 1741 gcttttgcaa aaagcttacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc 1801 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg 1861 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc 1921 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc 1981 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca 2041 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca 2101 actgcgtgca cttcgtggcc gaggagcagg actgaccgat cgataaaata aaagatttta 2161 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct 2221 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga 2281 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc 2341 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg 2401 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg 2461 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac 2521 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg 2581 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt 2641 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca 2701 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc 2761 agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat 2821 ttacattaaa tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 2881 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 2941 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 3001 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 3061 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 3121 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 3181 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 3241 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 3301 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 3361 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 3421 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 3481 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 3541 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 3601 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 3661 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 3721 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc 3781 gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 3841 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 3901 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 3961 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 4021 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 4081 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 4141 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 4201 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 4261 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 4321 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 4381 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 4441 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 4501 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 4561 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 4621 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 4681 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 4741 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 4801 gaaaagtgcc a //