LOCUS       Exported                7260 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pKH3-TRIM28
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Liang Q, Deng H, Li X, Wu X, Tang Q, Chang TH, Peng H, Rauscher FJ 
            3rd, Ozato K, Zhu F
  TITLE     Tripartite motif-containing protein 28 is a small ubiquitin-related 
            modifier E3 ligase and negative regulator of IFN regulatory factor 
            7.
  JOURNAL   J Immunol. 2011 Nov 1;187(9):4754-63. doi: 10.4049/jimmunol.1101704.
            Epub 2011 Sep 21.
  PUBMED    21940674
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     98..115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    232..253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        268..298
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    306..322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     311..333
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     330..346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     330..346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        375..754
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        755..958
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     909..929
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     955..979
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1189..1207
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1189..1206
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1220..1239
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1300..1326
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1333..1359
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1366..1392
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      3921..3930
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    3934..4068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3971..3990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4025..4044
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        4137..4494
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(4160..4180)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4345..4480
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4407..4426
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4514..4531)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4514..4530)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4523..4545)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4743..5198
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4830..4849)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5040..5061
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5215..5234)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5334..5356
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5394..5412)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5480..5584
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5585..6445
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5803..5822)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6616..7204
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7105..7124
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaatta
      361 attcgagctc gcccgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      421 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      481 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      541 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      601 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      661 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      721 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      781 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      841 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      901 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
      961 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     1021 acaccgggac cgatccagcc tccgcggccg ggaacggtgc attggaacgc ggattccccg
     1081 tgccaagagt gacgtaagta ccgcctatag agtctatagg cccaccccct tggcttcgtt
     1141 agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca
     1201 ctatagaata acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg
     1261 cacctcggtt ctaagcttct gcaggtcgac tctagaatgt acccatacga tgttccagat
     1321 tacgctggat cttacccata cgatgttcca gattacgctg gatcttaccc atacgatgtt
     1381 ccagattacg ctggatctat ggcggcctcc gcggcggcag cctcggcagc agcggcctcg
     1441 gccgcctctg gcagcccggg cccgggcgag ggctccgctg gcggcgaaaa gcgctccacc
     1501 gccccttcgg ccgcagcctc ggcctctgcc tcagccgcgg cgtcgtcgcc cgcggggggc
     1561 ggcgccgagg cgctggagct gctggagcac tgcggcgtgt gcagagagcg cctgcgaccc
     1621 gagagggagc cccgcctgct gccctgtttg cactcggcct gtagtgcctg cttagggccc
     1681 gcggcccccg ccgccgccaa cagctcgggg gacggcgggg cggcgggcga cggcaccgtg
     1741 gtggactgtc ccgtgtgcaa gcaacagtgc ttctccaaag acatcgtgga gaattatttc
     1801 atgcgtgata gtggcagcaa ggctgccacc gacgcccagg atgcgaacca gtgctgcact
     1861 agctgtgagg ataatgcccc agccaccagc tactgtgtgg agtgctcgga gcctctgtgt
     1921 gagacctgtg tagaggcgca ccagcgggtg aagtacacca aggaccatac tgtgcgctct
     1981 actgggccag ccaagtctcg ggatggtgaa cgtactgtct attgcaacgt acacaagcat
     2041 gaaccccttg tgctgttttg tgagagctgt gatactctca cctgccgaga ctgccagctc
     2101 aatgcccaca aggaccacca gtaccagttc ttagaggatg cagtgaggaa ccagcgcaag
     2161 ctcctggcct cactggtgaa gcgccttggg gacaaacatg caacattgca gaagagcacc
     2221 aaggaggttc gcagctcaat ccgccaggtg tctgacgtac agaagcgtgt gcaagtggat
     2281 gtcaagatgg ccatcctgca gatcatgaag gagctgaata agcggggccg tgtgctggtc
     2341 aatgatgccc agaaggtgac tgaggggcag caggagcgcc tggagcggca gcactggacc
     2401 atgaccaaga tccagaagca ccaggagcac attctgcgct ttgcctcttg ggctctggag
     2461 agtgacaaca acacagccct tttgctttct aagaagttga tctacttcca gctgcaccgg
     2521 gccctcaaga tgattgtgga tcccgtggag ccacatggcg agatgaaatt tcagtgggac
     2581 ctcaatgcct ggaccaagag tgccgaggcc tttggcaaga ttgtggcaga gcgtcctggc
     2641 actaactcaa caggccctgc acccatggcc cctccaagag ccccagggcc cctgagcaag
     2701 cagggctctg gcagcagcca gcccatggag gtgcaggaag gctatggctt tgggtcagga
     2761 gatgatccct actcaagtgc agagccccat gtgtcaggtg tgaaacggtc ccgctcaggt
     2821 gagggcgagg tgagcggcct tatgcgcaag gtgccacgag tgagccttga acgcctggac
     2881 ctggacctca cagctgacag ccagccaccc gtcttcaagg tcttcccagg cagtaccact
     2941 gaggactaca accttattgt tattgaacgt ggcgctgccg ctgcagctac cggccagcca
     3001 gggactgcgc ctgcaggaac ccctggtgcc ccacccctgg ctggcatggc cattgtcaag
     3061 gaggaggaga cggaggctgc cattggagcc cctcctactg ccactgaggg ccctgagacc
     3121 aaacctgtgc ttatggctct tgcggagggt cctggtgctg agggtccccg cctggcctca
     3181 cctagtggca gcaccagctc agggctggag gtggtggctc ctgagggtac ctcagcccca
     3241 ggtggtggcc cgggaaccct ggatgacagt gccaccattt gccgtgtctg ccagaagcca
     3301 ggcgatctgg ttatgtgcaa ccagtgtgag ttttgtttcc acctggactg tcacctgccg
     3361 gccctgcagg atgtaccagg ggaggagtgg agctgctcac tctgccatgt gctccctgac
     3421 ctgaaggagg aggatggcag cctcagcctg gatggtgcag acagcactgg cgtggtggcc
     3481 aagctctcac cagccaacca gcggaaatgt gagcgtgtac tgctggccct attctgtcac
     3541 gaaccctgcc gccccctgca tcagctggct accgactcca ccttctccct ggaccagccc
     3601 ggtggcaccc tggatctgac cctgatccgt gcccgcctcc aggagaagtt gtcacctccc
     3661 tacagctccc cacaggagtt tgcccaggat gtgggccgca tgttcaagca attcaacaag
     3721 ttaactgagg acaaggcaga cgtgcagtcc atcatcggcc tgcagcgctt cttcgagacg
     3781 cgcatgaacg aggccttcgg tgacaccaag ttctctgctg tgctggtgga gcccccgccg
     3841 atgagcctgc ctggtgctgg cctgagttcc caggagctgt ctggtggccc tggtgatggc
     3901 ccctgagaat tcaatcgatg gccgccatgg cccaacttgt ttattgcagc ttataatggt
     3961 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4021 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcgg gaattaattc
     4081 ggcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg taccttctga
     4141 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     4201 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4261 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4321 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4381 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     4441 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctg
     4501 ttaacagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     4561 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     4621 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     4681 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     4741 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4801 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4861 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4921 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     4981 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5041 tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt
     5101 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5161 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     5221 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     5281 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     5341 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     5401 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5461 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5521 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5581 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5641 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5701 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5761 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5821 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5881 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5941 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6001 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6061 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6121 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6181 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6241 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6301 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6361 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6421 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6481 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6541 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6601 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6661 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6721 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6781 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6841 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6901 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6961 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7021 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7081 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7141 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7201 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
//
