LOCUS Exported 12171 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION lentiviral expression of shRNA resistant Psd95-EGFP fusion. ACCESSION . VERSION . KEYWORDS FUp95GW (B7) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12171) AUTHORS Schluter OM, Xu W, Malenka RC TITLE Alternative N-terminal domains of PSD-95 and SAP97 govern activity-dependent regulation of synaptic AMPA receptor function. JOURNAL Neuron. 2006 Jul 6;51(1):99-111. PUBMED 16815335 REFERENCE 2 (bases 1 to 12171) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12171 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(46..65) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 237..616 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 618..816 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 771..791 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 834..1014 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1061..1186 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1679..1912 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2097..2141 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2439..2556 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2621..3832 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3669..3688 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" regulatory 6096..6105 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6102..6821 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6102..6818 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6147..6168) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6408..6427) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6755..6776 /label=EGFP-C /note="EGFP, forward primer" misc_feature 6862..7450 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6915..6935) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7333..7344) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(7453..7469) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7454..7470) /label=pBluescriptKS /note="For pBluescript vector" LTR 7975..8155 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(8181..8198) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8187..8411 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8457..8885 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8544..8563) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8754..8775 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(8894..8914) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8899..9228 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9079..9214 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9141..9160 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 9276..9323 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 9342..9716 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 9846..9967 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9883..9902) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9937..9956 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(10016..10032) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10016..10032) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10029..10051) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10040..10056 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10064..10094) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10109..10130 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10247..10264) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10418..11006) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10498..10517) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11177..12037) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11800..11819 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12038..12142) /gene="bla" /label=AmpR promoter ORIGIN 1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata 301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc 841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg 1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat 1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg 1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa 1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct 1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac 1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca 1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa 1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga 1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag 1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag 1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga 1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc 1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc 1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt 1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta 1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta 2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga 2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa 2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa 2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat 2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag 2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg 2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt 2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa 2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga 2581 gatccagttt ggttaattaa cccgtgtcgg ctccagatct ggcctccgcg ccgggttttg 2641 gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc 2701 agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac 2761 tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct 2821 agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc 2881 gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg 2941 ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat 3001 cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg 3061 ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc 3121 cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc 3181 ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg 3241 gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct 3301 tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga 3361 ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca 3421 gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt 3481 ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg 3541 caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg 3601 gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct 3661 taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga 3721 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta 3781 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg 3841 ggctgcaggt cgactctaga ggatccccgg gtaccggaat tcgccctttc tagaccacca 3901 tggactgtct ctgtatagtg acaaccaaga aataccgcta ccaagatgaa gacacgcccc 3961 ctctggaaca cagcccggcc cacctcccca accaggccaa ttctccccct gtgattgtca 4021 acacggacac cctagaagcc ccaggatatg agttgcaggt gaatggaaca gagggggaga 4081 tggagtatga ggagatcaca ttggaaaggg gtaactcagg tctgggcttc agcatcgcag 4141 gtggcactga caacccgcac atcggtgacg acccgtccat ttttatcacc aagatcattc 4201 ctggtggggc tgcagcccag gatggccgcc tcagggtcaa tgacagcatc ctgtttgtaa 4261 atgaagtgga cgtacgagag gtgacccatt cagctgcggt ggaggccctc aaagaggcag 4321 gttccatcgt tcgcctctat gtcatgcgcc ggaaaccccc agccgaaaag gtcatggaga 4381 tcaaactcat caaagggcct aaaggacttg gcttcagcat tgcggggggc gttgggaacc 4441 agcacatccc tggagataac agcatctatg taacgaagat catcgaagga ggtgctgccc 4501 acaaggatgg caggttgcag attggagaca agatcctggc ggtcaacagt gtggggctgg 4561 aggacgtcat gcacgaggat gccgtggcag ccctgaagaa cacatatgac gttgtgtacc 4621 taaaggtggc caagcccagc aatgcctacc tgagtgacag ctatgctccc ccagacatca 4681 caacctcgta ttctcagcac ctggacaatg agatcagtca tagcagctac ttgggcactg 4741 actaccccac agccatgacc cccacttccc ctcggcgcta ctcccctgtg gccaaggacc 4801 tgctggggga ggaagacatt ccccgggaac caaggcggat cgtgatccat cggggctcca 4861 ccggcctggg cttcaacatc gtgggcggcg aggatggtga aggcatcttc atctccttca 4921 tccttgctgg gggtccagcc gacctcagtg gggagctacg gaagggggac cagatcctgt 4981 cggtcaatgg tgttgacctc cgcaatgcca gtcacgaaca ggctgccatt gccctgaaga 5041 atgcgggtca gacggtcacg atcatcgctc agtataaacc agaagagtat agtcgattcg 5101 aggccaagat ccatgatctt cgggaacagc tcatgaatag tagcctaggc tcagggactg 5161 catccttgcg aagcaacccc aagaggggct tctacattag ggccctgttt gattacgaca 5221 agaccaagga ctgcggtttc ttgagccagg ccctgagctt ccgcttcggg gatgtgcttc 5281 atgtcattga cgctggtgac gaagagtggt ggcaagcacg gcgggtccac tccgacagtg 5341 agaccgacga cattggcttc attcccagca aacggcgggt cgagcgacga gagtggtcaa 5401 ggttaaaggc caaggactgg ggctccagct ctggatcaca gggtcgagaa gactcggttc 5461 tgagctatga gacggtgacc cagatggaag tgcactatgc tcgtcccatc atcatccttg 5521 gacccaccaa agaccgtgcc aacgatgatc ttctctccga gttccccgac aagtttggat 5581 cctgtgtccc tcatacgaca cgtcctaagc gggaatatga gatagacggc cgggattacc 5641 actttgtctc ctcccgggag aaaatggaga aggacatcca ggcacacaag ttcattgagg 5701 ctggccagta caacagccac ctctatggga ccagcgtcca gtctgtgcga gaggtagcag 5761 agcaggggaa gcactgcatc ctcgatgtct cggccaatgc cgtgcggcgg ctgcaggcgg 5821 cccacctgca ccccatcgcc atcttcatcc gtccccgctc cctggagaat gtgctagaga 5881 tcaataagcg gatcacagag gagcaagccc ggaaagcctt cgacagagcc acgaagctgg 5941 agcaggagtt cacagagtgc ttctcagcca tcgtagaggg cgacagcttt gaagagatct 6001 atcacaaagt gaaacgtgtc attgaagacc tctcaggccc ctacatctgg gtcccagccc 6061 gagagagact cggtaccgcg ggcccgggat ccatcgccac catggtgagc aagggcgagg 6121 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca 6181 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt 6241 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct 6301 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt 6361 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact 6421 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga 6481 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca 6541 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca 6601 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca 6661 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg 6721 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg 6781 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagaa 6841 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact 6901 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 6961 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 7021 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 7081 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 7141 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 7201 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca 7261 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 7321 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 7381 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc 7441 gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt 7501 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag 7561 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta 7621 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 7681 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag 7741 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta 7801 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta 7861 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt 7921 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct 7981 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 8041 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 8101 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc 8161 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 8221 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 8281 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 8341 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 8401 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 8461 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 8521 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 8581 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 8641 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 8701 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 8761 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 8821 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 8881 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 8941 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 9001 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 9061 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 9121 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta 9181 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga 9241 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt 9301 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg 9361 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc 9421 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga 9481 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt 9541 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc 9601 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg 9661 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg 9721 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 9781 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 9841 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 9901 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 9961 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 10021 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 10081 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 10141 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 10201 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 10261 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 10321 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 10381 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 10441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 10501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 10561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 10621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 10681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 10741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 10801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 10861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 10921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 10981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 11041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 11101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 11161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 11221 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 11281 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 11341 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 11401 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 11461 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 11521 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 11581 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 11641 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 11701 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 11761 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 11821 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 11881 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 11941 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 12001 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 12061 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 12121 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac g //