LOCUS Exported 4749 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Mitochondria, Excitation: 574, Emission: 596. ACCESSION . VERSION . KEYWORDS mStrawberry-Mito-7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4749) TITLE Davidson Red fluorescent proteins REFERENCE 2 (bases 1 to 4749) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4749 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 119..422 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 423..626 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 577..597 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 655..729 /codon_start=1 /product="mitochondrial presequence of human cytochrome c oxidase subunit VIII (Rizutto et al., 1989)" /label=COX8 presequence /translation="MSVLTPLLLRGLTGSARRLPVPRAK" CDS 763..1473 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein" /label=mStrawberry /note="mammalian codon-optimized" /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA EGRHSTGGMDELYK" primer_bind complement(908..926) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1176..1195 /label=mCherry-F /note="mCherry, forward primer" polyA_signal 1595..1716 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1632..1651) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1686..1705 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1723..2178) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1860..1881) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2072..2091 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2205..2309 /gene="bla" /label=AmpR promoter promoter 2311..2668 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2334..2354) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2519..2654 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2581..2600 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2703..3497 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2757..2776) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3367..3386 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3685..3704) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3729..3776 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4105..4693 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4594..4613 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta 61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat 481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac 601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcatgtcc 661 gtcctgacgc cgctgctgct gcggggcttg acaggctcgg cccggcggct cccagtgccg 721 cgcgccaaga tccattcgtt gggggatcca ccggtggcta ccatggtgag caagggcgag 781 gagaataaca tggccatcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc 841 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc 901 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatccta 961 acccccaact tcacctacgg ctccaaggcc tacgtgaagc accccgccga catccccgac 1021 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 1081 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag 1141 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg 1201 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc 1261 aagatgaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac 1321 aaggccaaga agcccgtgca gctgcccggc gcctacatcg tcggcatcaa gttggacatc 1381 acctcccaca acgaggacta caccatcgtg gaactgtacg aacgcgccga gggccgccac 1441 tccaccggcg gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag 1501 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 1561 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 1621 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 1681 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata 1741 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 1801 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 1861 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 1921 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 1981 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 2041 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 2101 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 2161 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 2221 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 2281 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt 2341 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 2401 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 2461 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 2521 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 2581 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 2641 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc 2701 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 2761 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 2821 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 2881 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 2941 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 3001 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 3061 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 3121 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 3181 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg 3241 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 3301 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 3361 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 3421 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 3481 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa 3541 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 3601 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 3661 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg 3721 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa 3781 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg 3841 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc 3901 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat 3961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 4021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 4081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 4141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 4201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 4261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 4321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 4381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 4441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 4501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 4561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 4621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 4681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 4741 ttttgctca //