LOCUS       Exported                6420 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Localization: Lysosomes, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    tdTomato-CathespinB-6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6420)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1708..1717
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1714..3144
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    3266..3387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3303..3322)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3357..3376
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3394..3849)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3531..3552)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3743..3762
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3876..3980
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3982..4339
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(4005..4025)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4190..4325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4252..4271
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4374..5168
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4428..4447)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5038..5057
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5356..5375)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5400..5447
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5776..6364
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6265..6284
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggactcagat ctcgagctca agcttcgaat tccaggatgt ggtggtcctt gatccctctc
      721 tcttgcctgc tggcactgac cagtgcccat gacaagcctt cctttcaccc actgtcggac
      781 gacatgatta actatatcaa caaacagaat acaacatggc aggctggacg caacttctac
      841 aatgttgaca taagctatct gaagaagctg tgtggaactg tcctgggtgg acccaagctg
      901 ccggaaaggg ttgggttcag cgaggacata aatctacctg aatcctttga tgcacgggaa
      961 cagtggtcca attgcccgac catcgcacag atcagagacc aggggtcctg tggctcttgt
     1021 tgggcatttg gggcagtgga agccatgtct gaccgaatct gcattcacac caatggccga
     1081 gtcaatgtgg aggtgtctgc tgaggacctg cttacctgct gtggtatcca gtgtggggat
     1141 ggctgtaatg gtggctatcc ctctggagca tggaacttct ggactagaaa aggcctggtt
     1201 tctggtggag tatacaattc tcatataggc tgcttaccct acaccatccc tccctgtgaa
     1261 caccatgtca atggctcccg tcccccatgc actggagaag gagatactcc caagtgcaac
     1321 aagatgtgtg aggctggcta ctccacatcc tacaaggaag ataagcacta tgggtacact
     1381 tcctacagtg tgtctgacag cgagaaggag atcatggcgg aaatctacaa aaatggccca
     1441 gtggagggtg cttttactgt gttttctgac ttcttgactt acaaatcagg cgtatacaag
     1501 catgaagccg gtgatgtgat gggaggccat gccatccgca ttctgggctg gggaatagag
     1561 aatggagtac cctactggct ggtagcaaac tcctggaacg ttgactgggg tgataatggt
     1621 ttctttaaaa tcctcagagg agagaaccac tgtggaattg aatcagaaat cgtggctgga
     1681 atcccacgca ctcaggatcc accggtcgcc accatggtga gcaagggcga ggaggtcatc
     1741 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag
     1801 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     1861 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc
     1921 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc
     1981 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc
     2041 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac
     2101 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     2161 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
     2221 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa
     2281 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
     2341 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct ggggcatggc
     2401 accggcagca ccggcagcgg cagctccggc accgcctcct ccgaggacaa caacatggcc
     2461 gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag
     2521 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     2581 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg
     2641 tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc
     2701 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc
     2761 gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc
     2821 accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     2881 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag
     2941 ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc
     3001 gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag
     3061 gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgtac
     3121 ggcatggacg agctgtacaa gtaagcggcc gcgactctag atcataatca gccataccac
     3181 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     3241 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     3301 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3361 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
     3421 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     3481 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     3541 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     3601 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     3661 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     3721 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     3781 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     3841 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3901 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3961 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     4021 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4081 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4141 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4201 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4261 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4321 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     4381 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     4441 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     4501 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     4561 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     4621 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     4681 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     4741 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     4801 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     4861 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     4921 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     4981 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     5041 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     5101 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     5161 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     5221 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     5281 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     5341 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     5401 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     5461 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     5521 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     5581 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
     5641 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5701 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5761 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5821 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5881 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5941 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6001 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6061 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6121 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6181 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6241 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6301 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6361 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
//
