LOCUS Exported 6211 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS GFP-C1-CAMKIIalpha SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6211) AUTHORS Shen K, Teruel MN, Subramanian K, Meyer T TITLE CaMKIIbeta functions as an F-actin targeting module that localizes CaMKIIalpha/beta heterooligomers to dendritic spines. JOURNAL Neuron. 1998 Sep . 21(3):593-606. PUBMED 9768845 REFERENCE 2 (bases 1 to 6211) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6211 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 607..616 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 613..1329 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 613..1329 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(658..679) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(919..938) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1266..1287 /label=EGFP-C /note="EGFP, forward primer" misc_feature 1330..1395 /label=MCS /note="multiple cloning site" polyA_signal 2999..3120 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3036..3055) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3090..3109 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(3127..3582) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3264..3285) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3476..3495 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3609..3713 /gene="bla" /label=AmpR promoter promoter 3715..4072 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3738..3758) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3923..4058 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3985..4004 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4107..4901 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4161..4180) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4771..4790 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(5089..5108) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5133..5180 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5509..6097 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5998..6017 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc 1381 gcgggcccgg gatccagtgc caggatggct accatcacct gcacccgatt cacggaagag 1441 taccagctct tcgaggaact gggaaaggga gccttctccg tggtgcgcag gtgtgtgaag 1501 gtgctggctg gccaggagta tgctgccaag attatcaaca ccaagaagct ctcagccaga 1561 gatcaccaga agttggaacg cgaggcccgc atctgccgct tgttgaagca ccccaatatc 1621 gtccgactcc atgacagcat ctccgaggag gggcaccact accttatctt cgatctggtc 1681 actggtgggg agctgttcga agacattgtg gcccgggagt attacagtga ggctgatgcc 1741 agccactgta tccagcagat cctggaggct gtgctacact gtcaccagat gggggtggtg 1801 catcgcgacc tgaagcctga gaatctgttg ctggcttcga agctcaaggg tgctgcggtg 1861 aagctggcag actttggcct ggccatagag gttgagggag agcagcaggc atggtttggg 1921 ttcgcaggga cacctggata cctctcccca gaagtgctgc ggaaggaccc atacgggaag 1981 cctgtggacc tgtgggcctg tggcgtcatc ctgtatatct tgctggttgg gtatccccca 2041 ttctgggatg aggaccagca ccgcctgtac cagcagatca aagctggtgc ctacgatttc 2101 ccatcaccag aatgggacac cgtcaccccg gaagccaagg atctgatcaa taagatgctg 2161 accatcaacc cgtccaaacg catcacggcc gctgaggctc tcaagcaccc ctggatctcg 2221 caccgctcca ctgtggcctc ctgcatgcac agacaggaga ccgtggactg cctgaagaag 2281 ttcaatgcca ggaggaaact gaagggagcc atcctcacca ctatgctggc caccaggaac 2341 ttctccggag ggaagagtgg aggaaacaag aagaatgatg gcgtgaagga atcctctgag 2401 agcaccaaca ccaccatcga ggatgaagac accaaagtgc gcaaacagga aattatcaaa 2461 gtgacagagc agctgatcga agccataagc aatggagact ttgaatccta cacgaagatg 2521 tgcgaccctg gaatgacagc ctttgaaccg gaggccctgg ggaacctggt cgagggcctg 2581 gactttcatc gattctattt tgaaaacctg tggtcccgga acagcaagcc cgtgcacacc 2641 accatcctga accctcacat ccacctgatg ggtgacgagt cagcctgcat cgcctacatc 2701 cgcatcactc agtacctgga tgcgggtggc atcccccgca cggcccagtc agaggagacc 2761 cgtgtctggc accgcaggga tggaaaatgg cagatcgtcc acttccacag atctggggcg 2821 ccctccgtcc tgccccattg aaggaccagg ccagggtccc tgcgctcttg cttcgccgaa 2881 ttctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 2941 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 3001 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 3061 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 3121 acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 3181 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 3241 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 3301 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 3361 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 3421 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 3481 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 3541 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg 3601 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 3661 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg 3721 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 3781 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 3841 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 3901 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 3961 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 4021 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc 4081 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 4141 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 4201 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 4261 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca 4321 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 4381 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 4441 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 4501 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 4561 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 4621 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 4681 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 4741 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 4801 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 4861 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 4921 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 4981 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 5041 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa 5101 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca 5161 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga 5221 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca 5281 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc 5341 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa 5401 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 5461 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 5521 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 5581 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 5641 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 5701 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 5761 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 5821 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 5881 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 5941 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 6001 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 6061 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 6121 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 6181 ttctgtggat aaccgtatta ccgccatgca t //