LOCUS       Exported                9831 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Mammalian expression of human JMJD2C with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-HA-GASC1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9831)
  AUTHORS   Cloos PA, Christensen J, Agger K, Maiolica A, Rappsilber J, Antal T,
            Hansen KH, Helin K
  TITLE     The putative oncogene GASC1 demethylates tri- and dimethylated 
            lysine 9 on histone H3.
  JOURNAL   Nature. 2006 Jul 20. 442(7100):307-11.
  PUBMED    16732293
REFERENCE   2  (bases 1 to 9831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9831
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(101..120)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    145..192
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        485..864
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        865..1068
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1019..1039
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1065..1089
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1180..1752
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             1832..1858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1871..1895
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5114..5138)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5237..5256)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5302..5357
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5356..5375)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5715..5731)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5715..5731)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5728..5750)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5739..5755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5763..5793)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5808..5829
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5946..5963)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6117..6705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6197..6216)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6876..7736)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7499..7518
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7737..7841)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7909..7927
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7965..7987)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8087..8106
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8300..8322
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8314..8331
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8315..8331
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8742..8887
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             8944..9738
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8998..9017)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9608..9627
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
       61 tggagttctt cgcccacccc gggagatggg ggaggctaac tgaaacacgg aaggagacaa
      121 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
      181 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
      241 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
      301 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaagccct gccatagcca
      361 cgggccccgt gggttaggga cggcggatcg cggccctcta gagagcttgg cccattgcat
      421 acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac attaccgcca
      481 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
      541 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      601 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      661 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      721 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      781 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      841 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      901 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      961 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     1021 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     1081 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
     1141 cgatccagcc tccggtcgac cgatcctgag aacttcaggg tgagtttggg gacccttgat
     1201 tgttctttct ttttcgctat tgtaaaattc atgttatatg gagggggcaa agttttcagg
     1261 gtgttgttta gaatgggaag atgtcccttg tatcaccatg gaccctcatg ataattttgt
     1321 ttctttcact ttctactctg ttgacaacca ttgtctcctc ttattttctt ttcattttct
     1381 gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt
     1441 tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa tcagggtata
     1501 ttatattgta cttcagcaca gttttagaga acaattgtta taattaaatg ataaggtaga
     1561 atatttctgc atataaattc tggctggcgt ggaaatattc ttattggtag aaacaactac
     1621 accctggtca tcatcctgcc tttctcttta tggttacaat gatatacact gtttgagatg
     1681 aggataaaat actctgagtc caaaccgggc ccctctgcta accatgttca tgccttcttc
     1741 tctttcctac agctcctggg caacgtgctg gttgttgtgc tgtctcatca ttttggcaaa
     1801 gaattcctcg acggatcaat tcaccatggc atacccatac gatgttccag attacgctag
     1861 cggatcatca acaagtttgt acaaaaaagc aggctccgaa ttcgccctta tcatggaggt
     1921 ggccgaggtg gaaagtcctc tgaaccccag ctgtaagata atgaccttca gaccctccat
     1981 ggaggagttc cgggagttca acaaatacct tgcatacatg gagtctaaag gagcccatcg
     2041 tgcgggtctt gcaaaggtga ttcctcctaa ggagtggaag ccaagacagt gctatgatga
     2101 cattgataat ttgctcattc cagcaccaat tcagcagatg gtcacagggc agtcaggact
     2161 gttcactcag tacaacatcc agaaaaaagc gatgactgtg aaggagttca ggcagctggc
     2221 caacagtggc aaatattgta ctccaagata cttggattac gaagatttgg agcgcaagta
     2281 ctggaagaac ttaacttttg tggcacctat ctatggtgca gatattaatg ggagcatata
     2341 tgatgagggt gtggatgaat ggaacatagc tcgcctcaat acagtcttgg atgtggttga
     2401 agaagagtgt ggcatttcta ttgagggtgt aaatacccca tatctctatt ttggcatgtg
     2461 gaagaccacg tttgcatggc acaccgaaga catggacctc tatagcatta attatctcca
     2521 ctttggagag cccaagtctt ggtatgctat acctccggag catggaaaac gacttgaaag
     2581 actagctcaa ggttttttcc caagcagctc ccaagggtgt gatgcatttc ttcgccacaa
     2641 gatgacattg atttctccat cagtattgaa gaaatatggt attccctttg acaagataac
     2701 ccaggaggct ggagaattca tgatcacttt cccatatggc taccatgctg gttttaatca
     2761 tggtttcaac tgtgcagaat ctacaaattt tgctactgtc agatggattg actatggaaa
     2821 agttgccaaa ttgtgcactt gcaggaaaga catggtgaag atttcaatgg atatctttgt
     2881 gaggaaattt cagccagaca gatatcagct ttggaaacaa ggaaaggata tatacaccat
     2941 tgatcacacg aagcctactc cagcatccac ccctgaagta aaagcatggc tgcagaggag
     3001 gaggaaagta agaaaagcat cccgaagctt ccagtgtgct aggtctacct ctaaaaggcc
     3061 taaggctgat gaggaagagg aagtgtcaga tgaagtcgat ggggcagagg tccctaaccc
     3121 cgactcagtc acagatgacc tcaaggtcag tgaaaagtca gaagcagcag tgaagctgag
     3181 gaacacagaa gcatcttcag aagaagagtc atctgctagc aggatgcagg tggagcagaa
     3241 tttatcagat catatcaaac tctcaggaaa cagctgctta agtacatctg taacagaaga
     3301 cataaaaact gaggatgaca aagcttatgc atatagaagt gtaccttcta tatccagtga
     3361 ggctgatggt tccattccat tgtctagtgg ctatgagaag cccgagaaat cagacccatc
     3421 cgagctttca tggccaaagt cacctgagtc atgctcatca gtggcagaga gtaatggtgt
     3481 gttaacagag ggagaagaga gtgatgtgga gagccatggg aatggccttg aacctgggga
     3541 aatcccagcg gtccccagtg gagagagaaa tagcttcaaa gtccccagta tagcagaggg
     3601 agagaacaaa acctctaaga gttggcgcca tccacttagc aggcctccag caagatctcc
     3661 gatgactctt gtgaagcagc aggcgccaag tgatgaagaa ttgcctgagg ttctgtccat
     3721 tgaggaggaa gtggaagaaa cagagtcttg ggcgaaacct ctcatccacc tttggcagac
     3781 gaagtcccct aacttcgcag ctgagcaaga ttataatgca acagtggcca ggatgaagcc
     3841 acactgtgcc atctgcactc tgctcatgcc gtaccacaag ccagatagca gcaatgaaga
     3901 aaatgatgct agatgggaga caaaattaga tgaagtcgtt acatcggagg gaaagactaa
     3961 gcccctcata ccagagatgt gttttattta tagtgaagaa aatatagaat attctccacc
     4021 caatgccttc cttgaagagg atggaacaag tctccttatt tcctgtgcaa agtgctgcgt
     4081 acgggttcat gcaagttgtt atggtattcc ttctcatgag atctgtgatg gatggctgtg
     4141 tgcccggtgc aaaagaaatg cgtggacagc agaatgctgt ctctgcaatt tgagaggagg
     4201 tgctcttaag caaacgaaga acaataggtg ggcccatgtc atgtgcgccg ttgcggtccc
     4261 ggaagttcga ttcactaatg tcccagaaag gacacaaata gatgtaggca gaataccttt
     4321 acagaggtta aaattgaaat gcatcttctg cagacaccgg gttaagaggg tctctggagc
     4381 ctgcatccag tgttcctacg gtcgctgccc ggcctccttc catgtcactt gtgcccatgc
     4441 tgctggggta ctgatggagc ctgatgattg gccttatgtg gtgaacatta catgctttcg
     4501 acataaggtc aaccccaacg tgaagtccaa ggcttgcgag aaggtcattt ccgtgggtca
     4561 aacggtcatc acgaagcatc ggaacacccg gtattacagt tgcagagtga tggctgtgac
     4621 atcgcagacc ttctatgagg tcatgtttga tgatggttcc tttagcagag acacatttcc
     4681 tgaggatatc gtgagccgag actgtctgaa gctgggccca cctgctgagg gagaagtcgt
     4741 ccaagtcaag tggcccgatg gcaaactcta tggagcaaaa tattttggat caaatattgc
     4801 ccacatgtac caggttgagt ttgaagatgg atcccagata gcaatgaaga gagaggacat
     4861 ctacacttta gatgaagagt tacccaagag agtgaaagct cgattttcca cagcctctga
     4921 catgcgattt gaagacacgt tttatggagc agacattatc caaggggaga gaaagagaca
     4981 aagagtgctg agctccaggt ttaagaatga atatgtggcc gaccctgtat accgcacttt
     5041 tttgaagagc tctttccaga agaagtgcca gaagagacag tagtctgcat acatcgctgc
     5101 aagggcgaat tcgacccagc tttcttgtac aaagtggttg atgatccgtc gaggaattca
     5161 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     5221 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     5281 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     5341 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     5401 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     5461 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     5521 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     5581 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     5641 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ggatcggccg
     5701 caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5761 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     5821 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     5881 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     5941 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6001 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6061 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6121 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6181 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6241 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6301 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6361 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6421 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6481 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6541 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6601 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6661 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6721 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6781 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6841 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6901 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6961 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7021 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7081 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7141 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7201 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7261 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7321 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7381 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7441 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7501 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7561 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7621 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7681 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7741 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7801 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7861 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     7921 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     7981 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     8041 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     8101 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     8161 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     8221 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     8281 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt
     8341 cacgctgccg caagcactca gggcgcaagg gctgctaaag gaagcggaac acgtagaaag
     8401 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa
     8461 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc
     8521 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg
     8581 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
     8641 ggcgcagggg atcaagatcc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg
     8701 tgtccccgga agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca
     8761 gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtcccaggt
     8821 ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc
     8881 cgcttaacag cgtcaacagc gtgccgcaga tctgatcaag agacaggatg aggatcgttt
     8941 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     9001 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     9061 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     9121 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     9181 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     9241 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     9301 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     9361 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     9421 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     9481 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     9541 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     9601 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     9661 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     9721 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     9781 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg g
//
