LOCUS Exported 9554 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Limb connective tissue specific inducible Cre. ACCESSION . VERSION . KEYWORDS Prrx1:TFPnls-T2a-Cre-Ert SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9554) TITLE Cre-inducible plasmids REFERENCE 2 (bases 1 to 9554) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9554 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(3..458) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(140..161) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 352..371 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 585..607 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 599..616 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 600..616 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 649..668 /label=T7 /note="T7 promoter, forward primer" promoter 649..667 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" intron 3455..3587 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 3570..3589 /label=pMT2-F /note="Synthetic intron, forward primer" primer_bind 3684..3703 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" regulatory 3751..3760 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3757..4464 /codon_start=1 /product="monomeric teal (cyan) fluorescent protein (Ai et al., 2006)" /label=mTFP1 /note="mammalian codon-optimized" /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA VARNSTDGMDELYK" CDS 4489..4509 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4513..4533 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4537..4557 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4579..4632 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 4642..5664 /codon_start=1 /product="site-specific recombinase" /label=Cre /note="Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences." /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG D" CDS 5677..6612 /codon_start=1 /product="mutated ligand-binding domain of the human estrogen receptor (Feil et al., 1997)" /label=ERT2 /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET DQSHLATAGSTSSHSLQKYYITGEAEGFPAT" primer_bind complement(6702..6721) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 6767..6822 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(6821..6840) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(7343..7363) /label=T3 /note="T3 promoter, forward primer" promoter complement(7343..7361) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(7405..7421) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7405..7421) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7418..7440) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7429..7445 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7453..7483) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7498..7519 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7636..7653) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7807..8395) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7887..7906) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8566..9426) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9189..9208 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9427..9531) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgtagggat aacagggtaa tgcgcgcgta atacgactca 661 ctatagggcg aattgggtac ccagaggtgt aaagtacttg agtaatttta cttgattact 721 gtacttaagt attatttttg gggattttta ctttacttga gtacaattaa aaatcaatac 781 ttttactttt acttaattac atttttttag aaaaaaaagt actttttact ccttacaatt 841 ttatttacag tcaaaaagta cttatttttt ggagatcact tgggcccggc tcgagcatgc 901 acgcgtgaat tcaataggag cctgtaatta cgttctagaa acctgtagca agaattcaat 961 aggagcctgt aattacgttc tagaaacctg tagcaagaat tcttgctaca ggtttctaga 1021 acaatggggg aggggaagtc agcaagtggc agtagttggt ttgcctctga cctttaggaa 1081 aataccagac atttgcattt tctatatatg ctagatgcta tatttgttat tatctaaaca 1141 aatttgctac acagatgtta gaaatgtatc taaatgttcc catagaaacc attaataccc 1201 aggttgatac tgtagcaatt ggttgatagg aacatgggta accagctgcg gtttttcaaa 1261 atgtgcctgc ttcctggaga aaaggcggaa tgcaaggaaa ggcacttaat gatatttctg 1321 gggatggtct ctgatctctt ttccttgaca gtcttctgga gtatcaacca tactggctgc 1381 aaacagaaac aaggatactt cttggaggca atatctaggg tgctagaagt cctgatcttt 1441 tactcaggat ttggtcacaa atggcaatcc tcacgtctga aagaaaaagg aatccatctg 1501 tgtaagtaaa gatggagcgc atgagcctcc agtctgagaa gatatgggag agactgatgc 1561 tgtctggcct taggaagaga gaaagggtgt ctcctttccc ctaagggagc agagagttcc 1621 agagacaccg gtggaaatat gccactttaa cctgaccatc gagtcgcttc cactccaggc 1681 actttaggct ctaggagtct caccgttggc atctccgaaa gcacctcagg atactcaagt 1741 ccccactctg tctggcccac ccacgggcaa gtccccagcg caagaaccag ctcttctggc 1801 ttagatgccc tccggcagac aaaccttcgc ggctcagagc ctgagctgcc gagcaggggc 1861 gaccagagca ctttggcggg gctgggggct gccggtcggg ggcacggcac gagtggctgg 1921 agcgaggcga ggggtggaaa aaggaggggg cggcgggtgg actaatgatg aaaaagtctc 1981 ctccatcctg gctccttaat taaatgcatg gaaagaaccg aggcgcgcac atctggtttc 2041 aatctacagc cctttgatgg catcaaatgt tcttttccca gatcagggct ggaagttctg 2101 ggctaactat ggccgtttgg agcacagaaa ccatttatac acactgggac ccttccttct 2161 tttcaattca atctcatagc tacaggagaa ggggaaatct agcaatcagg ggagcgaggg 2221 gaaacgtatg tatgtgtgtg tttaaagaac ataaaacgcc acaaataagc acatactatt 2281 ttacttactg gccatatagt aactcattcc taatgagact attaaagctg ttaccagatt 2341 ccaacaggtc atgaaaacac cgtccagcac cgctctgccc tctgtcaagc ctggtcatag 2401 acttcgacct tcctcagtct ctgtgtttgc cagggcctca ccgaactgga tacagattag 2461 cacagagccc accccacccc cagctcctcc tgctcccccc aacttcttca gccccagtgt 2521 tcctgtctgc tctgatctgg ggcagcctcg gctacgagca agaaattaga acattggcaa 2581 aggtagggga attatcagga cggactttct tctggtttaa tgaatatgta aattttctac 2641 tgtctgaagc ctggggattt ctggtggcag cgaaagtctc gaatccattc gaagctgaag 2701 acttctggat ctgcccaagc cgtctttaga tcgtagagag cctcccgtta ttatcgctct 2761 tgttatttta ttacattatt taataggctg tctctacaga gccaaacgag ctttaatagg 2821 cttgctcttt tttgccgttc agtctcagac tctccctgcg ctctccgcca aaacaaagct 2881 gcaggaaaag ttactggggg gcggtgcagc cgcagatttg actgttatag atgtgcatct 2941 tttccctgtt cctttttctt ttttcttttc ctaacaagtt tgccctcagc tgttatcaaa 3001 acatgcaaat gagatattcc aagaggatct caaacaaatc cggaggagtt aatgctgaaa 3061 caaaataatc agccccgttt taaagtgact gcgggtttcg ggcacttttc tctctgtgat 3121 actactgcaa gggaatgata attagacctt tattttaaaa atccaaaggg gctctctcct 3181 tagcttccct cccttttccc tccctccctc cttcctccct ccctcctcct ctcttgctcc 3241 ctccctctcc ctttctctct ggctctgatg ttggcaaagg ggttttctta atcagactgt 3301 tttttcggtc cgaggaaaag gaggtaggag attgtgatgg agaaaggggg tctgtaaaac 3361 gtcaggcgcc gcacaaaggc tttgccacgt aattacaggc tcctattgaa ttcgaatttt 3421 aagctgcaga agttggtcgt gaggcactgg gcaggtaagt atcaaggtta caagacaggt 3481 ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata 3541 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg tccactccca 3601 gttcaattac agctcttaaa attccgccac tagagcctct gctaaccatg ttcatgcctt 3661 cttctctttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg 3721 caaagaatta ttccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagacc 3781 acaatgggcg taatcaagcc cgacatgaag atcaagctga agatggaggg caacgtgaat 3841 ggccacgcct tcgtgatcga gggcgagggc gagggcaagc cctacgacgg caccaacacc 3901 atcaacctgg aggtgaagga gggagccccc ctgcccttct cctacgacat tctgaccacc 3961 gcgttcgcct acggcaacag ggccttcacc aagtaccccg acgacatccc caactacttc 4021 aagcagtcct tccccgaggg ctactcttgg gagcgcacca tgaccttcga ggacaagggc 4081 atcgtgaagg tgaagtccga catctccatg gaggaggact ccttcatcta cgagatacac 4141 ctcaagggcg agaacttccc ccccaacggc cccgtgatgc agaagaagac caccggctgg 4201 gacgcctcca ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga cgtcaagcac 4261 aagctgctgc tggagggcgg cggccaccac cgcgttgact tcaagaccat ctacagggcc 4321 aagaaggcgg tgaagctgcc cgactatcac tttgtggacc accgcatcga gatcctgaac 4381 cacgacaagg actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg caattccacc 4441 gacggcatgg acgagctgta caagtccgga ctcagatctc gagctgatcc aaaaaagaag 4501 agaaaggtag atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagga 4561 tccaccggat ctagacccga gggcagagga agtcttctaa catgcggtga cgtggaggag 4621 aatcccggcc cactagtcga caatttactg accgtacacc aaaatttgcc tgcattaccg 4681 gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag 4741 gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg 4801 tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt 4861 ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta 4921 aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg 4981 gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta 5041 gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc 5101 caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc 5161 gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc 5221 catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg 5281 ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat 5341 aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag 5401 ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct 5461 aaggatgact ccggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc 5521 gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg 5581 accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg 5641 gtgcgcctgc tggaagatgg cgatctcgag ccatctgctg gagacatgag agctgccaac 5701 ctttggccaa gcccgctcat gatcaaacgc tctaagaaga acagcctggc cttgtccctg 5761 acggccgacc agatggtcag tgccttgttg gatgctgagc cccccatact ctattccgag 5821 tatgatccta ccagaccctt cagtgaagct tcgatgatgg gcttactgac caacctggca 5881 gacagggagc tggttcacat gatcaactgg gcgaagaggg tgccaggctt tgtggatttg 5941 accctccatg atcaggtcca ccttctagaa tgtgcctggc tagagatcct gatgattggt 6001 ctcgtctggc gctccatgga gcacccagtg aagctactgt ttgctcctaa cttgctcttg 6061 gacaggaacc agggaaaatg tgtagagggc atggtggaga tcttcgacat gctgctggct 6121 acatcatctc ggttccgcat gatgaatctg cagggagagg agtttgtgtg cctcaaatct 6181 attattttgc ttaattctgg agtgtacaca tttctgtcca gcaccctgaa gtctctggaa 6241 gagaaggacc atatccaccg agtcctggac aagatcacag acactttgat ccacctgatg 6301 gccaaggcag gcctgaccct gcagcagcag caccagcggc tggcccagct cctcctcatc 6361 ctctcccaca tcaggcacat gagtaacaaa ggcatggagc atctgtacag catgaagtgc 6421 aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg cggacgccca ccgcctacat 6481 gcgcccacta gccgtggagg ggcatccgtg gaggagacgg accaaagcca cttggccact 6541 gcgggctcta cttcatcgca ttccttgcaa aagtattaca tcacggggga ggcagagggt 6601 ttccctgcca cagcttaaga tatcactcct caggtgcagg ctgcctatca gaaggtggtg 6661 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa 6721 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt 6781 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg 6841 agggcaaatc atttaaaaca tcagaatgag catttggttt agagtttggc aacatatgcc 6901 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag 6961 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt 7021 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta 7081 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta 7141 tgaagatccc tcgaccagcg gccgctaata ctcaagtaca attttaatgg agtacttttt 7201 tacttttact caagtaagat tctagccaga tacttttact tttaattgag taaaattttc 7261 cctaagtact tgtactttca cttgagtaaa atttttgagt actttttaca cctctgccgc 7321 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcatt accctgttat 7381 ccctacgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 7441 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 7501 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 7561 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 7621 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 7681 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 7741 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 7801 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 7861 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 7921 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 7981 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 8041 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 8101 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 8161 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 8221 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 8281 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 8341 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 8401 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 8461 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 8521 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 8581 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 8641 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 8701 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 8761 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 8821 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 8881 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 8941 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 9001 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 9061 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 9121 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 9181 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 9241 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 9301 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 9361 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 9421 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 9481 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 9541 ccgaaaagtg ccac //