LOCUS Exported 2859 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 6 aa C-terminal moiety, which consists of 2 Asn and 4 Gly. ACCESSION . VERSION . KEYWORDS r-6. pT7-7-SNCA-2Asn SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2859) AUTHORS Afitska K, Fucikova A, Shvadchak VV, Yushchenko DA TITLE Modification of C Terminus Provides New Insights into the Mechanism of alpha-Synuclein Aggregation. JOURNAL Biophys J. 2017 Nov 21;113(10):2182-2191. doi: 10.1016/j.bpj.2017.08.027. Epub 2017 Sep 20. PUBMED 28939194 REFERENCE 2 (bases 1 to 2859) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2859 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 132..151 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 385..402 /label=L4440 /note="L4440 vector, forward primer" primer_bind 637..659 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 724..743 /label=T7 /note="T7 promoter, forward primer" promoter 724..742 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" RBS 774..796 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS complement(1322..2182) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1945..1964 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2183..2287) /gene="bla" /label=AmpR promoter primer_bind 2355..2373 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" rep_origin 2502..231 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg 421 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg aagatcttcc 481 ggaagatctt cctatggtgc actctcagta caatctgctc tgatgcgcta cgtgactggg 541 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 601 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 661 tttcaccgtc atcaccgaaa cgcgcgaggc ccagcgattc gaacttctga tagacttcga 721 aattaatacg actcactata gggagaccac aacggtttcc ctctagaaat aattttgttt 781 aactttaaga aggagatata catatggatg tattcatgaa aggactttca aaggccaagg 841 agggagttgt ggctgctgct gagaaaacca aacagggtgt ggcagaagca gcaggaaaga 901 caaaagaggg tgttctctat gtaggctcca aaaccaagga gggagtggtg catggtgtgg 961 caacagtggc tgagaagacc aaagagcaag tgacaaatgt tggaggagca gtggtgacgg 1021 gtgtgacagc agtagcccag aagacagtgg agggagcagg gagcattgca gcagccactg 1081 gctttgtcaa aaaggaccag ttgggcaaga atgaagaagg agccccacag gaaggaattc 1141 tggaagatat gcctgtggat cctgacaatg aggcttatga aatgccttct gaggaagggt 1201 atcaagacta tgaacctgaa gccggcggca acggcaacgg ctaaaagctt atcgatgata 1261 agctgtcaaa catgagaatt aaatcaatct aaagtatata tgagtaaact tggtctgaca 1321 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 1381 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 1441 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 1501 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 1561 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 1621 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 1681 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 1741 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 1801 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 1861 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 1921 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc 1981 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 2041 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 2101 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 2161 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 2221 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 2281 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 2341 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa taaaaggatc 2401 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2461 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 2521 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2581 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2641 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2701 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 2761 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 2821 acggggggtt cgtgcacaca gcccagcttg gagcgaacg //