LOCUS       Exported                5504 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  An AAV vector that expresses green pre-eGRASP(p30) under the CaMKIIa
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CWB-pre-eGRASP(p30)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Choi JH, Sim SE, Kim JI, Choi DI, Oh J, Ye S, Lee J, Kim T, Ko HG, 
            Lim CS, Kaang BK
  TITLE     Interregional synaptic maps among engram cells underlie memory 
            formation.
  JOURNAL   Science. 2018 Apr 27;360(6387):430-435. doi: 
            10.1126/science.aas9204.
  PUBMED    29700265
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     regulatory      561..570
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             645..1103
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKRHDFFKSAMPEGYVQERTISFKDDGK
                     YKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITA"
     primer_bind     complement(684..705)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    1934..2522
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1987..2007)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2405..2416)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2535..2759
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2767..2907
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2767..2896
                     /label=AAV2 ITR
     rep_origin      2982..3437
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3069..3088)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3279..3300
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3454..3473)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3573..3595
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3633..3651)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3719..3823
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3824..4684
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4042..4061)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4855..5443
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5344..5363
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca gatgctgcac acttgtggac taagtttgtt
      181 cgcatcccct tctccaaccc cctcagtaca tcaccctggg ggaacagggt ccacttgctc
      241 ctgggcccac acagtcctgc agtattgtgt atataaggcc agggcaaaga ggagcaggtt
      301 ttaaagtgaa aggcaggcag gtgttgggga ggcagttacc ggggcaacgg gaacagggcg
      361 tttcggaggt ggttgccatg gggacctgga tgctgacgaa ggctcgcgag gctgtgagca
      421 gccacagtgc cctgctcaga agccccaagc tcgtcagtca agccggttct ccgtttgcac
      481 tcaggagcac gggcaggcga gtggccccta gttctggggg cagcgtgaaa agacggtatc
      541 gataagcttg attcgagcta gccaccatgg agaccgacac cctcctgcta tgggtgctgc
      601 tgctctgggt gcccggcagc accggcgacg caccggtcgg aggcagcaag ggcgaggagc
      661 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      721 tcagcgtgag gggcgagggc gagggcgatg ccaccatcgg caagctgacc ctgaagttca
      781 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
      841 gcgtgcagtg cttcgcccgc taccccgacc acatgaagcg gcacgacttc ttcaagtccg
      901 ccatgcccga aggctacgtc caggagcgca ccatctcctt caaggacgac ggcaagtaca
      961 agacccgcgc cgtggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1021 gcaccgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca
     1081 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaccg
     1141 tgcgccacaa cgtggaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1201 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagaccgtgc
     1261 tgagcaaaga ccccaacgag aagaccggtg gatctggagg cagtggcgga tctagagctc
     1321 ctacaaagcc tccaccactg ccacctggag gaggttccgg aggcggaagc ggtaccgaag
     1381 tgccttcctc tatgacaact gagtcgacag ccactgccat gcagtccgag atgtccacct
     1441 caatcatgga gaccaccaca accctggcta ccagcacagc tcgacgagga aagcccccca
     1501 caaaggagcc tatcagccag accacggatg atatccttgt ggcctcggca gagtgtccca
     1561 gcgacgatga ggacattgac ccctgtgagc cgagctcagg tgggttagcc aaccccaccc
     1621 gagtgggcgg ccgcgaacca tacccaggct cggcagaggt gatccgggag tctagcagta
     1681 ccactggcat ggtggtgggg attgtcgcag cagcagctct gtgcatcctc atcctcctct
     1741 atgccatgta caagtacaga aaccgggatg aaggctcata tcacgtggat gagagtcgaa
     1801 actacatcag taactcagca cagtccaatg gggctgtggt caaggagaag cagcccagca
     1861 gtgcgaaaag cgccaacaaa aacaagaaga acaaggataa ggagtattat gtctgaattc
     1921 ctgcagcccc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     1981 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     2041 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     2101 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     2161 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     2221 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     2281 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     2341 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     2401 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     2461 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     2521 gcatcggggg gatcctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     2581 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     2641 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     2701 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     2761 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     2821 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     2881 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     2941 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     3001 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3061 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3121 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3181 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3241 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3301 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     3361 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     3421 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3481 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     3541 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     3601 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     3661 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     3721 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3781 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3841 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3901 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3961 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4021 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4081 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4141 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4201 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4261 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4321 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4381 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4441 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4501 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4561 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4621 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4681 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4741 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4801 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4861 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4921 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4981 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     5041 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5101 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5161 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5221 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5281 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5341 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5401 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5461 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
