LOCUS Exported 5504 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION An AAV vector that expresses green pre-eGRASP(p32) under the CaMKIIa promoter.. ACCESSION . VERSION . KEYWORDS pAAV-CWB-pre-eGRASP(p32) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5504) AUTHORS Choi JH, Sim SE, Kim JI, Choi DI, Oh J, Ye S, Lee J, Kim T, Ko HG, Lim CS, Kaang BK TITLE Interregional synaptic maps among engram cells underlie memory formation. JOURNAL Science. 2018 Apr 27;360(6387):430-435. doi: 10.1126/science.aas9204. PUBMED 29700265 REFERENCE 2 (bases 1 to 5504) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5504 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..141 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 12..141 /label=AAV2 ITR regulatory 561..570 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 645..1103 /codon_start=1 /product="improved N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VN155(I152L) /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKF ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKRHDFFKSAMPEGYVQERTISFKDDGK YKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITA" primer_bind complement(684..705) /label=EGFP-N /note="EGFP, reverse primer" misc_feature 1934..2522 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(1987..2007) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2405..2416) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 2535..2759 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 2767..2907 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 2767..2896 /label=AAV2 ITR rep_origin 2982..3437 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3069..3088) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3279..3300 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3454..3473) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3573..3595 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3633..3651) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3719..3823 /gene="bla" /label=AmpR promoter CDS 3824..4684 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4042..4061) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4855..5443 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5344..5363 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca gatgctgcac acttgtggac taagtttgtt 181 cgcatcccct tctccaaccc cctcagtaca tcaccctggg ggaacagggt ccacttgctc 241 ctgggcccac acagtcctgc agtattgtgt atataaggcc agggcaaaga ggagcaggtt 301 ttaaagtgaa aggcaggcag gtgttgggga ggcagttacc ggggcaacgg gaacagggcg 361 tttcggaggt ggttgccatg gggacctgga tgctgacgaa ggctcgcgag gctgtgagca 421 gccacagtgc cctgctcaga agccccaagc tcgtcagtca agccggttct ccgtttgcac 481 tcaggagcac gggcaggcga gtggccccta gttctggggg cagcgtgaaa agacggtatc 541 gataagcttg attcgagcta gccaccatgg agaccgacac cctcctgcta tgggtgctgc 601 tgctctgggt gcccggcagc accggcgacg caccggtcgg aggcagcaag ggcgaggagc 661 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 721 tcagcgtgag gggcgagggc gagggcgatg ccaccatcgg caagctgacc ctgaagttca 781 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg 841 gcgtgcagtg cttcgcccgc taccccgacc acatgaagcg gcacgacttc ttcaagtccg 901 ccatgcccga aggctacgtc caggagcgca ccatctcctt caaggacgac ggcaagtaca 961 agacccgcgc cgtggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 1021 gcaccgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca 1081 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaccg 1141 tgcgccacaa cgtggaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 1201 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagaccgtgc 1261 tgagcaaaga ccccaacgag aagaccggtg gatctggagg cagtggcgga tctagatctc 1321 ctagctactc tccaccacct ccacctggag gaggttccgg aggcggaagc ggtaccgaag 1381 tgccttcctc tatgacaact gagtcgacag ccactgccat gcagtccgag atgtccacct 1441 caatcatgga gaccaccaca accctggcta ccagcacagc tcgacgagga aagcccccca 1501 caaaggagcc tatcagccag accacggatg atatccttgt ggcctcggca gagtgtccca 1561 gcgacgatga ggacattgac ccctgtgagc cgagctcagg tgggttagcc aaccccaccc 1621 gagtgggcgg ccgcgaacca tacccaggct cggcagaggt gatccgggag tctagcagta 1681 ccactggcat ggtggtgggg attgtcgcag cagcagctct gtgcatcctc atcctcctct 1741 atgccatgta caagtacaga aaccgggatg aaggctcata tcacgtggat gagagtcgaa 1801 actacatcag taactcagca cagtccaatg gggctgtggt caaggagaag cagcccagca 1861 gtgcgaaaag cgccaacaaa aacaagaaga acaaggataa ggagtattat gtctgaattc 1921 ctgcagcccc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 1981 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 2041 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 2101 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 2161 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 2221 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 2281 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt 2341 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 2401 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 2461 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 2521 gcatcggggg gatcctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 2581 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 2641 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 2701 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 2761 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca 2821 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga 2881 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc 2941 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc 3001 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 3061 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 3121 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 3181 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt 3241 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 3301 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc 3361 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 3421 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 3481 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc 3541 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc 3601 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt 3661 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg 3721 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 3781 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 3841 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 3901 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 3961 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 4021 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 4081 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 4141 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 4201 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 4261 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 4321 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 4381 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 4441 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 4501 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 4561 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 4621 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 4681 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 4741 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 4801 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 4861 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 4921 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 4981 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 5041 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 5101 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 5161 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 5221 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 5281 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 5341 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 5401 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 5461 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt //