LOCUS Exported 2895 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses human WT alpha synuclein with a C-terminal cysteine. ACCESSION . VERSION . KEYWORDS pT7-7 aSyn C141 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2895) TITLE Bacterial expression plasmids and lentivirus expression vectors for tau and alpha-synuclein JOURNAL Unpublished REFERENCE 2 (bases 1 to 2895) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2895 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 119..136 /label=L4440 /note="L4440 vector, forward primer" primer_bind 371..393 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 458..477 /label=T7 /note="T7 promoter, forward primer" promoter 458..476 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" RBS 508..530 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS complement(1092..1952) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1715..1734 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1953..2057) /gene="bla" /label=AmpR promoter primer_bind 2125..2143 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" rep_origin 2272..2860 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2761..2780 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 61 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 121 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct 181 gtgcggtatt tcacaccgca taggaagatc ttccggaaga tcttcctatg gtgcactctc 241 agtacaatct gctctgatgc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca 301 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 361 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 421 aggcccagcg attcgaactt ctgatagact tcgaaattaa tacgactcac tatagggaga 481 ccacaacggt ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg 541 gatgtattca tgaaaggact ttcaaaggcc aaggagggag ttgtggctgc tgctgagaaa 601 accaaacagg gtgtggcaga agcagcagga aagacaaaag agggtgttct ctatgtaggc 661 tccaaaacca aggagggagt ggtgcatggt gtggcaacag tggctgagaa gaccaaagag 721 caagtgacaa atgttggagg agcagtggtg acgggtgtga cagcagtagc ccagaagaca 781 gtggagggag cagggagcat tgcagcagcc actggctttg tcaaaaagga ccagttgggc 841 aagaatgaag aaggagcccc acaggaagga attctggaag atatgcctgt ggatcctgac 901 aatgaggctt atgaaatgcc ttctgaggaa gggtatcaag actatgaacc tgaagcctgc 961 taagaaatat ctttgctcca agtttcttga gatctgctga cagatgttcc atccaagctt 1021 atcgatgata agctgtcaaa catgagaatt aaatcaatct aaagtatata tgagtaaact 1081 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 1141 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 1201 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 1261 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 1321 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 1381 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 1441 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 1501 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 1561 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 1621 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 1681 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact 1741 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 1801 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 1861 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 1921 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 1981 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 2041 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 2101 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa 2161 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 2221 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 2281 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 2341 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 2401 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 2461 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 2521 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 2581 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 2641 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 2701 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 2761 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 2821 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 2881 tttacggttc ctggc //