LOCUS Exported 10443 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).. ACCESSION . VERSION . KEYWORDS pcDNA3.1 IGF1R-BirA(R118G)-HA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10443) AUTHORS Bareja A, Hodgkinson CP, Soderblom E, Waitt G, Dzau VJ TITLE The proximity-labeling technique BioID identifies sorting nexin 6 as a member of the insulin-like growth factor 1 (IGF1)-IGF1 receptor pathway. JOURNAL J Biol Chem. 2018 Mar 12. pii: RA118.002406. doi: 10.1074/jbc.RA118.002406. PUBMED 29530981 REFERENCE 2 (bases 1 to 10443) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10443 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 629..648 /label=T7 /note="T7 promoter, forward primer" promoter 629..647 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 4780..5739 /codon_start=1 /product="promiscuous R118G mutant of the E. coli biotin protein ligase (Cronan, 2005)" /label=BirA(R118G) /note="also known as BirA* or BioID" /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK" CDS 5743..5769 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" primer_bind complement(5800..5817) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5806..6030 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6076..6504 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6163..6182) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6373..6394 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6513..6533) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6518..6847 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6698..6833 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6760..6779 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 6914..7708 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6968..6987) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7578..7597 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 7882..8003 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7919..7938) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7973..7992 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8052..8068) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8052..8068) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8065..8087) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8076..8092 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8100..8130) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8145..8166 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8283..8300) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8454..9042) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8534..8553) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9213..10073) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9836..9855 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10074..10178) /gene="bla" /label=AmpR promoter primer_bind complement(10253..10272) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag 601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg 661 gctagcatga agtctggctc cggaggaggg tccccgacct cgctgtgggg gctcctgttt 721 ctctccgccg cgctctcgct ctggccgacg agtggagaaa tctgcgggcc aggcatcgac 781 atccgcaacg actatcagca gctgaagcgc ctggagaact gcacggtgat cgagggctac 841 ctccacatcc tgctcatctc caaggccgag gactaccgca gctaccgctt ccccaagctc 901 acggtcatta ccgagtactt gctgctgttc cgagtggctg gcctcgagag cctcggagac 961 ctcttcccca acctcacggt catccgcggc tggaaactct tctacaacta cgccctggtc 1021 atcttcgaga tgaccaatct caaggatatt gggctttaca acctgaggaa cattactcgg 1081 ggggccatca ggattgagaa aaatgctgac ctctgttacc tctccactgt ggactggtcc 1141 ctgatcctgg atgcggtgtc caataactac attgtgggga ataagccccc aaaggaatgt 1201 ggggacctgt gtccagggac catggaggag aagccgatgt gtgagaagac caccatcaac 1261 aatgagtaca actaccgctg ctggaccaca aaccgctgcc agaaaatgtg cccaagcacg 1321 tgtgggaagc gggcgtgcac cgagaacaat gagtgctgcc accccgagtg cctgggcagc 1381 tgcagcgcgc ctgacaacga cacggcctgt gtagcttgcc gccactacta ctatgccggt 1441 gtctgtgtgc ctgcctgccc gcccaacacc tacaggtttg agggctggcg ctgtgtggac 1501 cgtgacttct gcgccaacat cctcagcgcc gagagcagcg actccgaggg gtttgtgatc 1561 cacgacggcg agtgcatgca ggagtgcccc tcgggcttca tccgcaacgg cagccagagc 1621 atgtactgca tcccttgtga aggtccttgc ccgaaggtct gtgaggaaga aaagaaaaca 1681 aagaccattg attctgttac ttctgctcag atgctccaag gatgcaccat cttcaagggc 1741 aatttgctca ttaacatccg acgggggaat aacattgctt cagagctgga gaacttcatg 1801 gggctcatcg aggtggtgac gggctacgtg aagatccgcc attctcatgc cttggtctcc 1861 ttgtccttcc taaaaaacct tcgcctcatc ctaggagagg agcagctaga agggaattac 1921 tccttctacg tcctcgacaa ccagaacttg cagcaactgt gggactggga ccaccgcaac 1981 ctgaccatca aagcagggaa aatgtacttt gctttcaatc ccaaattatg tgtttccgaa 2041 atttaccgca tggaggaagt gacggggact aaagggcgcc aaagcaaagg ggacataaac 2101 accaggaaca acggggagag agcctcctgt gaaagtgacg tcctgcattt cacctccacc 2161 accacgtcga agaatcgcat catcataacc tggcaccggt accggccccc tgactacagg 2221 gatctcatca gcttcaccgt ttactacaag gaagcaccct ttaagaatgt cacagagtat 2281 gatgggcagg atgcctgcgg ctccaacagc tggaacatgg tggacgtgga cctcccgccc 2341 aacaaggacg tggagcccgg catcttacta catgggctga agccctggac tcagtacgcc 2401 gtttacgtca aggctgtgac cctcaccatg gtggagaacg accatatccg tggggccaag 2461 agtgagatct tgtacattcg caccaatgct tcagttcctt ccattccctt ggacgttctt 2521 tcagcatcga actcctcttc tcagttaatc gtgaagtgga accctccctc tctgcccaac 2581 ggcaacctga gttactacat tgtgcgctgg cagcggcagc ctcaggacgg ctacctttac 2641 cggcacaatt actgctccaa agacaaaatc cccatcagga agtatgccga cggcaccatc 2701 gacattgagg aggtcacaga gaaccccaag actgaggtgt gtggtgggga gaaagggcct 2761 tgctgcgcct gccccaaaac tgaagccgag aagcaggccg agaaggagga ggctgaatac 2821 cgcaaagtct ttgagaattt cctgcacaac tccatcttcg tgcccagacc tgaaaggaag 2881 cggagagatg tcatgcaagt ggccaacacc accatgtcca gccgaagcag gaacaccacg 2941 gccgcagaca cctacaacat caccgacccg gaagagctgg agacagagta ccctttcttt 3001 gagagcagag tggataacaa ggagagaact gtcatttcta accttcggcc tttcacattg 3061 taccgcatcg atatccacag ctgcaaccac gaggctgaga agctgggctg cagcgcctcc 3121 aacttcgtct ttgcaaggac tatgcccgca gaaggagcag atgacattcc tgggccagtg 3181 acctgggagc caaggcctga aaactccatc tttttaaagt ggccggaacc tgagaatccc 3241 aatggattga ttctaatgta tgaaataaaa tacggatcac aagttgagga tcagcgagaa 3301 tgtgtgtcca gacaggaata caggaagtat ggaggggcca agctaaaccg gctaaacccg 3361 gggaactaca cagcccggat tcaggccaca tctctctctg ggaatgggtc gtggacagat 3421 cctgtgttct tctatgtcca ggccaaaaca ggatatgaaa acttcatcca tctgatcatc 3481 gctctgcccg tcgctgtcct gttgatcgtg ggagggttgg tgattatgct gtacgtcttc 3541 catagaaaga gaaataacag caggctgggg aatggagtgc tgtatgcctc tgtgaacccg 3601 gagtacttca gcgctgctga tgtgtacgtt cctgatgagt gggaggtggc tcgggagaag 3661 atcaccatga gccgggaact tgggcagggg tcgtttggga tggtctatga aggagttgcc 3721 aagggtgtgg tgaaagatga acctgaaacc agagtggcca ttaaaacagt gaacgaggcc 3781 gcaagcatgc gtgaaaggat tgagtttctc aacgaagctt ctgtgatgaa ggagttcaat 3841 tgtcaccatg tggtgcgatt gctgggtgtg gtgtcccaag gccagccaac actggtcatc 3901 atggaactga tgacacgggg cgatctcaaa agttatctcc ggtctctgag gccagaaatg 3961 gagaataatc cagtcctagc acctccaagc ctgagcaaga tgattcagat ggccggagag 4021 attgcagacg gcatggcata cctcaacgcc aataagttcg tccacagaga ccttgctgcc 4081 cggaattgca tggtagccga agatttcaca gtcaaaatcg gagattttgg tatgacgcga 4141 gatatctatg agacagacta ttaccggaaa ggagggaaag ggctgctgcc cgtgcgctgg 4201 atgtctcctg agtccctcaa ggatggagtc ttcaccactt actcggacgt ctggtccttc 4261 ggggtcgtcc tctgggagat cgccacactg gccgagcagc cctaccaggg cttgtccaac 4321 gagcaagtcc ttcgcttcgt catggagggc ggccttctgg acaagccaga caactgtcct 4381 gacatgctgt ttgaactgat gcgcatgtgc tggcagtata accccaagat gaggccttcc 4441 ttcctggaga tcatcagcag catcaaagag gagatggagc ctggcttccg ggaggtctcc 4501 ttctactaca gcgaggagaa caagctgccc gagccggagg agctggacct ggagccagag 4561 aacatggaga gcgtccccct ggacccctcg gcctcctcgt cctccctgcc actgcccgac 4621 agacactcag gacacaaggc cgagaacggc cccggccctg gggtgctggt cctccgcgcc 4681 agcttcgacg agagacagcc ttacgcccac atgaacgggg gccgcaagaa cgagcgggcc 4741 ttgccgctgc cccagtcttc gacctgcgaa ttcggatcca aggacaacac cgtgcccctg 4801 aagctgatcg ccctgctggc caacggcgag ttccactctg gcgagcagct gggagagacc 4861 ctgggaatga gcagagccgc catcaacaag cacatccaga cactgagaga ctggggagtg 4921 gacgtgttca ccgtgcctgg caagggctac agcctgcctg agcctatcca gctgctgaac 4981 gccaagcaga tcctgggaca gctggatggc ggaagcgtgg ccgtgctgcc tgtgatcgac 5041 tccaccaatc agtacctgct ggacagaatc ggagagctga agtccggcga cgcctgcatc 5101 gccgagtacc agcaggctgg cagaggaggc agaggacgga agtggttcag cccattcgga 5161 gccaacctgt acctgtccat gttctggaga ctggagcagg gacctgctgc tgccatcgga 5221 ctgagtctgg tgatcggaat cgtgatggcc gaggtgctga gaaagctggg agccgacaag 5281 gtgagagtga agtggcctaa tgacctgtac ctccaggacc gcaagctggc tggcatcctg 5341 gtggagctga caggcaagac aggcgatgcc gctcagatcg tgatcggagc cggaatcaac 5401 atggccatga gaagagtgga ggagagcgtg gtgaaccagg gctggatcac cctgcaggag 5461 gctggcatca acctggaccg gaacaccctg gccgccatgc tgatcagaga gctgagagcc 5521 gctctggagc tgttcgagca ggagggactg gctccttacc tgagcagatg ggagaagctg 5581 gacaacttca tcaacagacc tgtgaagctg atcatcggcg acaaggaaat cttcggcatc 5641 tccagaggaa tcgacaagca gggagctctg ctgctggagc aggacggaat catcaagccc 5701 tggatgggcg gagaaatctc cctgagaagc gcagagaagg cttaccctta cgatgtaccg 5761 gattacgcat aggcggccta agtttaaacc gctgatcagc ctcgactgtg ccttctagtt 5821 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 5881 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 5941 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca 6001 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct 6061 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 6121 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 6181 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 6241 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 6301 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 6361 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 6421 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 6481 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa 6541 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 6601 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 6661 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 6721 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 6781 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 6841 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg 6901 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 6961 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 7021 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 7081 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 7141 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 7201 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 7261 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 7321 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 7381 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 7441 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 7501 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 7561 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 7621 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 7681 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa 7741 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 7801 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 7861 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 7921 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 7981 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg 8041 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 8101 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 8161 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 8221 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 8281 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 8341 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 8401 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 8461 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 8521 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 8581 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 8641 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 8701 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 8761 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 8821 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 8881 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 8941 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 9001 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 9061 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 9121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 9181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 9241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 9301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 9361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 9421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 9481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 9541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 9601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 9661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 9721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 9781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 9841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 9901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 9961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 10021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 10081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 10141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 10201 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct 10261 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg 10321 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga 10381 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc 10441 gtt //