LOCUS Exported 10758 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Bacterial Target-AID vector. ACCESSION . VERSION . KEYWORDS pScI_dCas9-CDA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10758) AUTHORS Banno S, Nishida K, Arazoe T, Mitsunobu H, Kondo A TITLE Deaminase-mediated multiplex genome editing in Escherichia coli. JOURNAL Nat Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 10.1038/s41564-017-0102-6. PUBMED 29403014 REFERENCE 2 (bases 1 to 10758) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10758 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 1905..1927 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 1919..1936 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 1920..1936 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" terminator 1971..2017 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(2025..2654) /codon_start=1 /product="cytidine deaminase from the sea lamprey Petromyzon marinus (Rogozin et al., 2007)" /label=PmCDA1 /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAVT" CDS complement(2661..2726) /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS complement(2745..2810) /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS complement(3018..7121) /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" RBS 7227..7235 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" CDS 7240..7953 /codon_start=1 /gene="cIts" /product="temperature-sensitive variant of the phage lambda repressor" /label=-lambda repressor (ts) /note="thermosensitivity is conferred by the A67T mutation" /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY PVFSHVQAEMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV VGKVIASQWPEETFG" CDS complement(8082..8741) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 8656..8675 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(8795..8825) /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS complement(8989..9939) /codon_start=1 /gene="rep101 encoding an A56V mutation" /product="temperature-sensitive version of the RepA protein needed for replication with the pSC101 origin (Armstrong et al., 1984)" /label=Rep101(Ts) /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTMKE RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" rep_origin 9987..10209 /label=pSC101 ori /note="low-copy replication origin that requires the Rep101 protein" primer_bind 10301..10319 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(10357..10379) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 10479..10498 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 cgacgcttta tatagaaaag aagattcaac taggtaaaat cttaatatag gttgagatga 61 taaggtttat aaggaatttg tttgttctaa tttttcactc attttgttct aatttctttt 121 aacaaatgtt cttttttttt tagaacagtt atgatatagt tagaatagtt taaaataagg 181 agtgagaaaa agatgaaaga aagatatgga acagtctata aaggctctca gaggctcata 241 gacgaagaaa gtggagaagt catagaggta gacaagttat accgtaaaca aacgtctggt 301 aacttcgtaa aggcatatat agtgcaatta ataagtatgt tagatatgat tggcggaaaa 361 aaacttaaaa tcgttaacta tatcctagat aatgtccact taagtaacaa tacaatgata 421 gctacaacaa gagaaatagc aaaagctaca ggaacaagtc tacaaacagt aataacaaca 481 cttaaaatct tagaagaagg aaatattata aaaagaaaaa ctggagtatt aatgttaaac 541 cctgaactac taatgagagg cgacgaccaa aaacaaaaat acctcttact cgaatttggg 601 aactttgagc aagaggcaaa tgaaatagat tgacctccca ataacaccac gtagttattg 661 ggaggtcaat ctatgaaatg cgattaagct ttttctaatt cacataagcg tgcaggttta 721 aagtacataa aaaatataat gaaaaaaagc atcattatac taacgttata ccaacattat 781 actctcatta tactaattgc ttattccaat ttcctattgg ttggaaccaa caggcgttag 841 tgtgttgttg agttggtact ttcatgggat taatcccatg aaacccccaa ccaactcgcc 901 aaagctctat attgataaaa ataataatag tgggtataat taagttgtta gagaaaacgt 961 ataaattagg agggattcat gctggtatga acaaaaatat aaaatattct caaaactttt 1021 taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata 1081 ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta 1141 aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa 1201 aactgaatac tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca 1261 aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa 1321 aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca 1381 agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc 1441 agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct 1501 taataaaact tacccgccat accacagatg ttccagataa atattggaag ctatatacgt 1561 actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc 1621 atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat 1681 tgtctatttt taatagttat ctattattta acgggaggaa ataaatgcat aaactgcatc 1741 ccttaacttg tttttcgtgt acctattttt tgtgaatcgc cattcgccat tcaggctgcg 1801 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 1861 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 1921 taaaacgacg gccagtgaat tcgagctcgg tacccggccg caaacaacag ataaaacgaa 1981 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctgtcaagt aacagcagga 2041 ctcttagtgg tgtggagtat ttttacctga atcataatgg acaactcgct ccgtcgtttt 2101 tcagctcgct tcaaagtctt ctcaagccat ctattctcat tcaattgatt gtgcgacgat 2161 tggatgaata ttttcctgca acattggtag tgttcactta ccattacatt caacccaacc 2221 ccgttatctc tgaggttcca cagcccaatt tgattcctcg catttttctc gtaatagagt 2281 ttgcaagccc agattttcaa agtgtggccg ttcccccgca gctcctggtt ataccattct 2341 aagatctttt cagcgcaatc tgcacaagga ctccaggatg agtaccaatt tatcgtgaat 2401 tgtccggggt tgtcgcgcag gtattcttcg acttttctaa tgctaaagat ttcggcgtga 2461 atgccacgtt ctgtcccgct ctgtggttta ttcacagcat agccccaaaa acacgctcta 2521 cgttcacccc gtcgttttaa ttcaaagaga acgtagcatc tatgcgacac ggattttttg 2581 ttgttgaaaa actgtttctt aaacgtgtag atgtccaact tctcatggat tctcacgtac 2641 tcagcgtcgg tcatcctaga cttatcgtca tcgtctttgt aatcaatatc atgatccttg 2701 tagtctccgt cgtggtcctt atagtctccg gactcgagcc tagacttatc gtcatcgtct 2761 ttgtaatcaa tatcatgatc cttgtagtct ccgtcgtggt ccttatagtc tccggaatac 2821 ttctccacgt aagggacagg aatcatcccc ctctttcctt cgctgtcctc tgcattccac 2881 cactgctcct caggcttatc ccggattctc aggatgtctc ctttcttaaa gggaagatcc 2941 tcttcatcat tcccattaaa gtcaaagagg gctcgcacat actcagcaga acctccacct 3001 ccagaacctc ctccaccgtc acctcctagc tgactcaaat caatgcgtgt ttcataaaga 3061 ccagtgatgg attgatggat aagagtggca tctaaaactt cttttgtaga cgtatatcgt 3121 ttacgatcaa ttgttgtatc aaaatattta aaagcagcgg gagctccaag attcgtcaac 3181 gtaaataaat gaataatatt ttctgcttgt tcacgtattg gtttgtctct atgtttgtta 3241 tatgcactaa gaactttatc taaattggca tctgctaaaa taacacgctt agaaaattca 3301 ctgatttgct caataatctc atctaaataa tgcttatgct gctccacaaa caattgtttt 3361 tgttcgttat cttctggact acccttcaac ttttcataat gactagctaa atataaaaaa 3421 ttcacatatt tgcttggcag agccagctca tttccttttt gtaattctcc ggcactagcc 3481 agcatccgtt tacgaccgtt ttctaactca aaaagactat atttaggtag tttaatgatt 3541 aagtcttttt taacttcctt atatccttta gcttctaaaa agtcaatcgg atttttttca 3601 aaggaacttc tttccataat tgtgatccct agtaactctt taacggattt taacttcttc 3661 gatttccctt tttccacctt agcaaccact aggactgaat aagctaccgt tggactatca 3721 aaaccaccat atttttttgg atcccagtct tttttacgag caataagctt gtccgaattt 3781 ctttttggta aaattgactc cttggagaat ccgcctgtct gtacttctgt tttcttgaca 3841 atattgactt ggggcatgga caatactttg cgcactgtgg caaaatctcg ccctttatcc 3901 cagacaattt ctccagtttc cccattagtt tcgattagag ggcgtttgcg aatctctcca 3961 tttgcaagtg taatttctgt tttgaagaag ttcatgatat tagagtaaaa gaaatatttt 4021 gcggttgctt tgcctatttc ttgctcagac ttagcaatca ttttacgaac atcataaact 4081 ttataatcac catagacaaa ctccgattca agttttggat atttcttaat caaagcagtt 4141 ccaacgacgg catttagata cgcatcatgg gcatgatggt aattgttaat ctcacgtact 4201 ttatagaatt ggaaatcttt tcggaagtca gaaactaatt tagattttaa ggtaatcact 4261 ttaacctctc gaataagttt atcattttca tcgtatttag tattcatgcg actatccaaa 4321 atttgtgcca catgcttagt gatttggcga gtttcaacca attggcgttt gataaaacca 4381 gctttatcaa gttcactcaa acctccacgt tcagctttcg ttaaattatc aaacttacgt 4441 tgagtgatta acttggcgtt tagaagttgt ctccaatagt ttttcatctt tttgactact 4501 tcttcacttg gaacgttatc cgatttacca cgatttttat cagaacgcgt taagacctta 4561 ttgtctattg aatcgtcttt aaggaaactt tgtggaacaa tggcatcgac atcataatca 4621 cttaaacgat taatatctaa ttcttggtcc acatacatgt ctcttccatt ttggagataa 4681 tagagataga gcttttcatt ttgcaattga gtattttcaa caggatgctc tttaagaatc 4741 tgacttccta attctttgat accttcttcg attcgtttca tacgctctcg cgaatttttc 4801 tggccctttt gagttgtctg attttcacgt gccatttcaa taacgatatt ttctggctta 4861 tgccgcccca ttactttgac caattcatca acaactttta cagtctgtaa aatacctttt 4921 ttaatagcag ggctaccagc taaatttgca atatgttcat gtaaactatc gccttgtcca 4981 gacacttgtg ctttttgaat gtcttcttta aatgtcaaac tatcatcatg gatcagctgc 5041 ataaaattgc gattggcaaa accatctgat ttcaaaaaat ctaatattgt tttgccagat 5101 tgcttatccc taataccatt aatcaatttt cgagacaaac gtccccaacc agtataacgg 5161 cgacgtttaa gctgtttcat caccttatca tcaaagaggt gagcatatgt tttaagtctt 5221 tcctcaatca tctccctatc ttcaaataag gtcaatgtta aaacaatatc ctctaagata 5281 tcttcatttt cttcattatc caaaaaatct ttatctttaa taatttttag caaatcatgg 5341 taggtaccta atgaagcatt aaatctatct tcaactcctg aaatttcaac actatcaaaa 5401 cattctattt ttttgaaata atcttctttt aattgcttaa cggttacttt tcgatttgtt 5461 ttgaagagta aatcaacaat ggctttcttc tgttcacctg aaagaaatgc tggttttcgc 5521 attccttcag taacatattt gacctttgtc aattcgttat aaaccgtaaa atactcataa 5581 agcaaactat gttttggtag tactttttca tttggaagat ttttatcaaa gtttgtcatg 5641 cgttcaataa atgattgagc tgaagcacct ttatcgacaa cttcttcaaa attccatggg 5701 gtaattgttt cttcagactt ccgagtcatc catgcaaaac gactattgcc acgcgccaat 5761 ggaccaacat aataaggaat tcgaaaagtc aagatttttt caatcttctc acgattgtct 5821 tttaaaaatg gataaaagtc ttcttgtctt ctcaaaatag catgcagctc acccaagtga 5881 atttgatggg gaatagagcc gttgtcaaag gtccgttgct tgcgcagcaa atcttcacga 5941 tttagtttca ccaataattc ctcagtacca tccatttttt ctaaaattgg tttgataaat 6001 ttataaaatt cttcttggct agctccccca tcaatataac ctgcatatcc gttttttgat 6061 tgatcaaaaa agatttcttt atacttttct ggaagttgtt gtcgaactaa agcttttaaa 6121 agagtcaagt cttgatgatg ttcatcgtag cgtttaatca ttgaagctga taggggagcc 6181 ttagttattt cagtatttac tcttaggata tctgaaagta aaatagcatc tgataaattc 6241 ttagctgcca aaaacaaatc agcatattga tctccaattt gcgccaataa attatctaaa 6301 tcatcatcgt aagtatcttt tgaaagctgt aatttagcat cttctgccaa atcaaaattt 6361 gatttaaaat taggggtcaa acccaatgac aaagcaatga gattcccaaa taagccattt 6421 ttcttctcac cggggagctg agcaatgaga ttttctaatc gtcttgattt actcaatcgt 6481 gcagaaagaa tcgctttagc atctactcca cttgcgttaa tagggttttc ttcaaataat 6541 tgattgtagg tttgtaccaa ctggataaat agtttgtcca catcactatt atcaggattt 6601 aaatctccct caatcaaaaa atgaccacga aacttaatca tatgcgctaa ggccaaatag 6661 attaagcgca aatccgcttt atcagtagaa tctaccaatt tttttcgcag atgatagata 6721 gttggatatt tctcatgata agcaacttca tctactatat ttccaaaaat aggatgacgt 6781 tcatgcttct tgtcttcttc caccaaaaaa gactcttcaa gtcgatgaaa gaaactatca 6841 tctactttcg ccatctcatt tgaaaaaatc tcctgtagat aacaaatacg attcttccga 6901 cgtgtatacc ttctacgagc tgtccgtttg agacgagtcg cttccgctgt ctctccactg 6961 tcaaataaaa gagcccctat aagatttttt ttgatactgt ggcggtctgt atttcccaga 7021 accttgaact ttttagacgg aaccttatat tcatcagtga tcaccgccca tccgacgcta 7081 tttgtgccga tagctaagcc tattgagtat ttcttatcca tttttgcctc ctaaaatggg 7141 ccctgcaacc attatcaccg ccagaggtaa aatagtcaac acgcgcggtg ttagatattt 7201 atcccttgcg gtgatagatt taacgttaag gaggtatgta tgagcacaaa aaagaaacca 7261 ttaacacaag agcagcttga ggacgcacgt cgccttaaag caatttatga aaaaaagaaa 7321 aatgaacttg gcttatccca ggaatctgtc gcagacaaga tggggatggg gcagtcaggc 7381 gttggtgctt tatttaatgg catcaatgca ttaaatgctt ataacgccgc attgcttaca 7441 aaaattctca aagttagcgt tgaagaattt agcccttcaa tcgccagaga aatctacgag 7501 atgtatgaag cggttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt 7561 tctcatgttc aggcagagat gttctcacct aagcttagaa cctttaccaa aggtgatgcg 7621 gagagatggg taagcacaac caaaaaagcc agtgattctg cattctggct tgaggttgaa 7681 ggtaattcca tgaccgcacc aacaggctcc aagccaagct ttcctgacgg aatgttaatt 7741 ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct gcatagccag acttgggggt 7801 gatgagttta ccttcaagaa actgatcagg gatagcggtc aggtgttttt acaaccacta 7861 aacccacagt acccaatgat cccatgcaat gagagttgtt ccgttgtggg gaaagttatc 7921 gctagtcagt ggcctgaaga gacgtttggc tgatcggcaa ggtgttctgg tcggcgcata 7981 gctgataaca attgagcaag aatcttcatc gctagaccgc aaaaatcagc gcgcaaatac 8041 gcatactgtt atctggcttt tagtaagccg gatccacgcg tttacgcccc gccctgccac 8101 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg 8161 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg 8221 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg 8281 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg 8341 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc 8401 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa 8461 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata 8521 cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac 8581 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg 8641 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg 8701 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct 8761 gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga agcataaagt 8821 gtaaagcctg gggtgcctaa tgcggcccct ctagctaccg gttttggtca tgagattatc 8881 tctgtgcata tggacagttt tccctttgat atgtaacggt gaacagttgt tctacttttg 8941 tttgttagtc ttgatgcttc actgatagat acaagagcca taagaacctc agatccttcc 9001 gtatttagcc agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga 9061 accattgaga tcatacttac tttgcatgtc actcaaaaat tttgcctcaa aactggtgag 9121 ctgaattttt gcagttaaag catcgtgtag tgtttttctt agtccgttat gtaggtagga 9181 atctgatgta atggttgttg gtattttgtc accattcatt tttatctggt tgttctcaag 9241 ttcggttacg agatccattt gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg 9301 gcggcctcgc ttatcaacca ccaatttcat attgctgtaa gtgtttaaat ctttacttat 9361 tggtttcaaa acccattggt taagcctttt aaactcatgg tagttatttt caagcattaa 9421 catgaactta aattcatcaa ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt 9481 tagttctttt aataaccact cataaatcct catagagtat ttgttttcaa aagacttaac 9541 atgttccaga ttatatttta tgaatttttt taactggaaa agataaggca atatctcttc 9601 actaaaaact aattctaatt tttcgcttga gaacttggca tagtttgtcc actggaaaat 9661 ctcaaagcct ttaaccaaag gattcctgat ttccacagtt ctcgtcatca gctctctggt 9721 tgctttagct aatacaccat aagcattttc cctactgatg ttcatcatct gaacgtattg 9781 gttataagtg aacgataccg tccgttcttt cattgtaggg ttttcaatcg tggggttgag 9841 tagtgccaca cagcataaaa ttagcttggt ttcatgctcc gttaagtcat agcgactaat 9901 cgctagttca tttgctttga aaacaactaa ttcagacata catctcaatt ggtctaggtg 9961 attttaatca ctataccaat tgagatgggc tagtcaatga taattactag tccttttcct 10021 ttgagttgtg ggtatctgta aattctgcta gacctttgct ggaaaacttg taaattctgc 10081 tagaccctct gtaaattccg ctagaccttt gtgtgttttt tttgtttata ttcaagtggt 10141 tataatttat agaataaaga aagaataaaa aaagataaaa agaatagatc ccagccctgt 10201 gtataactca ctactttagt cagttccgac gtgcacattt ccccgaaaag tgccacctga 10261 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 10321 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 10381 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 10441 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 10501 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 10561 caggcgatta tgtcttttgc gcattcactt cttttctata taaatatgag cgaagcgaat 10621 aagcgtcgga aaagcagcaa aaagtttcct ttttgctgtt ggagcatggg ggttcagggg 10681 gtgcagtatc tgacgtcaat gccgagcgaa agcgagccga agggtagcat ttacgttaga 10741 taaccccctg atatgctc //