LOCUS Exported 2887 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION PVanCC-YFP Reporter. Van sensor for use in Marionette strain.. ACCESSION . VERSION . KEYWORDS pAJM.714 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2887) AUTHORS Meyer AJ, Segall-Shapiro TH, Glassey E, Zhang J, Voigt CA TITLE Escherichia coli ""Marionette"" strains with 12 highly optimized small-molecule sensors. JOURNAL Nat Chem Biol. 2018 Nov 26. pii: 10.1038/s41589-018-0168-3. doi: 10.1038/s41589-018-0168-3. PUBMED 30478458 REFERENCE 2 (bases 1 to 2887) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2887 /organism="synthetic DNA construct" /mol_type="other DNA" misc_RNA 1..50 /label=sTRSV HHRz /note="hammerhead ribozyme from the tobacco ringspot virus satellite RNA (Khvorova et al., 2003)" CDS 100..819 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKLHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(145..166) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(406..425) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 753..774 /label=EGFP-C /note="EGFP, forward primer" rep_origin complement(1214..1759) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind complement(1294..1313) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1848..2663) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 2571..2590 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 2655..2674 /label=pENTR-R /note="pENTR vectors, reverse primer" ORIGIN 1 ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag cctctacaaa 61 taattttgtt taatactaga gaaagagggg aaatactaga tggtgagcaa gggcgaggag 121 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 181 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 241 atctgcacca caggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac 301 ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc tgcacgactt cttcaagtcc 361 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 421 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 481 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac 541 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag 601 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 661 ccaatcggcg acggccccgt gctgctgccc gacaaccact accttagcta ccagtccgcc 721 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 781 gccgggatca ctctcggcat ggacgagctg tacaagtaac tcggtaccaa attccagaaa 841 agaggcctcc cgaaaggggg gccttttttc gttttggtcc aatggcggcg cgccatcgaa 901 tgaggtgctt cctcgctcac tgactcgctg cacgaggcag acctcagcgc tagcggagtg 961 tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc atgtggcagg 1021 agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata ttccgcttcc 1081 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa 1141 cggggcggag atttcctgga agatgccagg aagatactta acagggaagt gagagggccg 1201 cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct 1261 caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg 1321 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat 1381 ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag 1441 ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat 1501 cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat 1561 tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag 1621 ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag 1681 aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg 1741 cagaccaaaa cgatctcaag aagatcatct tattaagggg tctgacgctc agtggaacga 1801 aaaatcaatc taaagtatat atgagtaaac ttggtctgac agttacctta gaaaaactca 1861 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga 1921 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga 1981 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc 2041 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag 2101 aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg 2161 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga 2221 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc 2281 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc 2341 tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg 2401 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc 2461 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca 2521 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc 2581 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct ggagcaagac 2641 gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt 2701 tttattgttc atgatgatat atttttatct tgtgcaatgt acatcagaga ttttgagaca 2761 caaccaatta ttgaaggcct ccctaacggg gggccttttt ttgtttctgg tctcccgctt 2821 aacgatcgtt ggctgattgg atccaattga cagctagctc agtcctaggt accattggat 2881 ccaatag //