LOCUS Exported 5637 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581. ACCESSION . VERSION . KEYWORDS mEos4b-Annexin-12 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5637) TITLE Davidson Photoactivatable Fluorescent Proteins REFERENCE 2 (bases 1 to 5637) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5637 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 54..357 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 358..561 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 512..532 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 600..609 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 606..1286 /codon_start=1 /product="fixation-resistant truly monomeric green-to-red photoswitchable fluorescent protein (Paez-Segala et al., 2015)" /label=mEos4b /note="derived from mEos2" /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP DNARR" polyA_signal 2418..2539 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2455..2474) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2509..2528 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2546..3001) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2683..2704) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2895..2914 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3028..3132 /gene="bla" /label=AmpR promoter promoter 3134..3491 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3157..3177) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3342..3477 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3404..3423 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3526..4320 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3580..3599) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4190..4209 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4508..4527) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4552..4599 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4928..5516 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5417..5436 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 61 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 121 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 181 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 241 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 301 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 361 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 421 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 481 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 541 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg 601 ccaccatggt gagtgcgatt aagccagaca tgaggatcaa actccgtatg gaaggcaacg 661 taaacgggca ccactttgtg atcgacggag atggtacagg caagccttat gagggaaaac 721 agaccatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga 781 ccactgcatt ccattacggc aacagggtat tcgtgaaata tccagacaac atacaagact 841 attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact ttcgaagacg 901 ggggcatttg caatgccaga aacgacataa caatggaagg ggacactttc tataataaag 961 ttcgatttta tggtaccaac tttcccgcca atggtccagt tatgcagaag aagacgctga 1021 aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgacg ggtgatattg 1081 agatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga actacttaca 1141 aagctaagga gaagggtgtc aagttaccag gcgcccactt tgtggaccac gccattgaga 1201 ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct gttgctcatt 1261 ctggattgcc tgacaatgcc agacgatccg gactcagatc tcgagctcaa gcttcgaatt 1321 ctatggccat ggcaaccaaa ggaggtactg tcaaagctgc ttcaggattc aatgccatgg 1381 aagatgccca gaccctgagg aaggccatga aagggctcgg caccgatgaa gacgccatta 1441 ttagcgtcct tgcctaccgc aacaccgccc agcgccagga gatcaggaca gcctacaaga 1501 gcaccatcgg cagggacttg atagacgacc tgaagtcaga actgagtggc aacttcgagc 1561 aggtgattgt ggggatgatg acgcccacgg tgctgtatga cgtgcaagag ctgcgaaggg 1621 ccatgaaggg agccggcact gatgagggct gcctaattga gatcctggcc tcccggaccc 1681 ctgaggagat ccggcgcata agccaaacct accagcagca atatggacgg agccttgaag 1741 atgacattcg ctctgacaca tcgttcatgt tccagcgagt gctggtgtct ctgtcagctg 1801 gtgggaggga tgaaggaaat tatctggacg atgctctcgt gagacaggat gcccaggacc 1861 tgtatgaggc tggagagaag aaatggggga cagatgaggt gaaatttcta actgttctct 1921 gttcccggaa ccgaaatcac ctgttgcatg tgtttgatga atacaaaagg atatcacaga 1981 aggatattga acagagtatt aaatctgaaa catctggtag ctttgaagat gctctgctgg 2041 ctatagtaaa gtgcatgagg aacaaatctg catattttgc tgaaaagctc tataaatcga 2101 tgaagggctt gggcaccgat gataacaccc tcatcagagt gatggtttct cgagcagaaa 2161 ttgacatgtt ggatatccgg gcacacttca agagactcta tggaaagtct ctgtactcgt 2221 tcatcaaggg tgacacatct ggagactaca ggaaagtact gcttgttctc tgtggaggag 2281 atgattaagg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag 2341 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 2401 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 2461 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 2521 aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg 2581 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct 2641 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt 2701 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat 2761 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca 2821 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac 2881 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta 2941 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg 3001 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 3061 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 3121 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg 3181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 3241 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 3301 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 3361 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 3421 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 3481 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 3541 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 3601 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 3661 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg 3721 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 3781 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 3841 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 3901 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 3961 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 4021 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc 4081 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 4141 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 4201 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 4261 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 4321 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 4381 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 4441 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga 4501 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa 4561 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc 4621 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt 4681 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg 4741 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa 4801 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 4861 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 4921 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 4981 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 5041 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 5101 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 5161 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 5221 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 5281 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 5341 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 5401 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 5461 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 5521 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 5581 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttat //