LOCUS       Exported                9477 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses a 1100-nt firefly luciferase circular RNA in Drosophila 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    Hy_pMT dati MCS circfirefly_1100
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9477)
  AUTHORS   Huang C, Liang D, Tatomer DC, Wilusz JE
  TITLE     A length-dependent evolutionarily conserved pathway controls nuclear
            export of circular RNAs.
  JOURNAL   Genes Dev. 2018 May 17. pii: gad.314856.118. doi: 
            10.1101/gad.314856.118.
  PUBMED    29773557
REFERENCE   2  (bases 1 to 9477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9477
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..427
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     polyA_signal    2782..2903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3613..3630)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3784..4372)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3864..3883)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4543..5403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5166..5185
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5404..5508)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5576..5594
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6990..7269
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     CDS             7315..8340
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     intron          8703..8768
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8898..8918
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9343..9477
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aattcgttgc aggacaggat gtggtgcccg atgtgactag ctctttgctg caggccgtcc
       61 tatcctctgg ttccgataag agacccagaa ctccggcccc ccaccgccca ccgccacccc
      121 catacatatg tggtacgcaa gtaagagtgc ctgcgcatgc cccatgtgcc ccaccaagag
      181 ctttgcatcc catacaagtc cccaaagtgg agaaccgaac caattcttcg cgggcagaac
      241 aaaagcttct gcacacgtct ccactcgaat ttggagccgg ccggcgtgtg caaaagaggt
      301 gaatcgaacg aaagacccgt gtgtaaagcc gcgtttccaa aatgtataaa accgagagca
      361 tctggccaat gtgcatcagt tgtggtcagc agcaaaatca agtgaatcat ctcagtgcaa
      421 ctaaaggggg gatctcgagc ctctgccatg actatcgtag agtaaaaagg gttaagcatt
      481 tccgaccata taaagtataa atactcttga tcagaatcaa tagccgagtc gaactggcca
      541 tgtccgcctc aacctatgag gattgaaaca agcattcatt ctgaatacat agacgcagcg
      601 caagtttgtt gactcatatt gtcacactca ctctaacgcc cacaaagcgc cttaacctgc
      661 cacggacacc tttttgaaat tcgaaatatt tcgttatctt ttcatatttt tattagtttt
      721 gtaaatttat atcgagttgc aaaaaaaact attccacgcc aactataact cccacaaacc
      781 gcccaaaaat gccacaccca ctcttttaag aaatgattca attttgttgg attaaataag
      841 tcgttttcct tatttcaatc tatatgccga aaacatattg accacgccca ctctgacccc
      901 taaaaccgcc caattttaaa aattcaaaaa acccttttac gcttaacaac tttttttgtt
      961 ttacaggcag gtaccatgga agatgccaaa aacattaaga agggcccagc gccattctac
     1021 ccactcgaag acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg
     1081 gtgcccggca ccatcgcctt taccgacgca catatcgagg tggacattac ctacgccgag
     1141 tacttcgaga tgagcgttcg gctggcagaa gctatgaagc gctatgggct gaatacaaac
     1201 catcggatcg tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc
     1261 ctgttcatcg gtgtggctgt ggccccagct aacgacatct acaacgagcg cgagctgctg
     1321 aacagcatgg gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag
     1381 atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat ggatagcaag
     1441 accgactacc agggcttcca aagcatgtac accttcgtga cttcccattt gccacccggc
     1501 ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg acaaaaccat cgccctgatc
     1561 atgaacagta gtggcagtac cggattgccc aagggcgtag ccctaccgca ccgcaccgct
     1621 tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca accagatcat ccccgacacc
     1681 gctatcctca gcgtggtgcc atttcaccac ggcttcggca tgttcaccac gctgggctac
     1741 ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg aggaggagct attcttgcgc
     1801 agcttgcaag actataagat tcaatctgcc ctgctggtgc ccacactatt tagcttcttc
     1861 gctaagagca ctctcatcga caagtacgac ctaagcaact tgcacgagat cgccagcggc
     1921 ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca aacgcttcca cctaccaggc
     1981 atccgccagg gctacggcct gacagaaaca accagcgcca ttctgatcac ccccgaaggg
     2041 gacgacaagc ccccggggag ctccaggtat tgttttgaag atttttctgc ttatctatat
     2101 actttaaagg gaacctggta atcgaatatt cgatgcagtc caaacaagag tagtaaaaaa
     2161 tcaacagaga acgctattcg agtttttaga ctagactata tgtacacccg atatttagct
     2221 gttacgagcg caacaatgaa ctttcaaaat tgtgtgactg ttttggacgt tttgcaggtg
     2281 taagtagggg cgtggccaaa gtgtttttgg tatgtcaata aaaattggca agacaatgaa
     2341 aacgaagatt aatcaaaaca ttttttgtgg gggcgtggca aacagttttt tggcaaatcg
     2401 attttatttt atcgatgaaa taatatgaat acattttgca agagcgtggg agtggcagtt
     2461 ctggacgctt tgtgggcgtt agaggatgcg tagcaacaaa cttgcgctgc gatctatgct
     2521 taatctcaac tttctagctt ttagttagat agtttctgaa atctcgacgt tcatacaggc
     2581 ggatatggcc agttcgactc gtcttttgat gctaatcaag aatatatgta gtttatatgg
     2641 tcggaaacgc ttccttctac ctgttacata cctttaaaca aatctaatat acgctttccc
     2701 tctacgagta atgggtatga aaacaaaaat caacacgcat caacgcattt tggaaagttg
     2761 aaggcgtgat agtgcggccg caacttgttt attgcagctt ataatggtta caaataaagc
     2821 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     2881 tccaaactca tcaatgtatc ttaactagtg gatccactag gggccgccga cgcgaggctg
     2941 gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca
     3001 ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc
     3061 ggctcttacc agcctaactt cgatcattgg accgctgatc gtcacggcga tttatgccgc
     3121 ctcggcgagc acatggaacg ggttggcatg gattgtaggc gccgccctat accttgtctg
     3181 cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa tggaagccgg
     3241 cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga
     3301 actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc
     3361 agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg
     3421 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa
     3481 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca
     3541 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg
     3601 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3661 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3721 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3781 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3841 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3901 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3961 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4021 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4081 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4141 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4201 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     4261 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4321 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4381 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4441 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4501 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4561 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4621 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4681 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4741 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4801 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc
     4861 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4921 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4981 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5041 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5101 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
     5161 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5221 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5281 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5341 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5401 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5461 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5521 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     5581 gcgtatcacg aggccctttc gtcttcaaga actcacattt gtacgaattt tttttttatc
     5641 aaaagttcga gtttttcacc aatttcctca tcaaccgagc aaggcaaacg gctttgaata
     5701 atatggtgtt atatatacat atatcaaatc gctgctgact gcgtgattga tggccccaag
     5761 attacatatt atcgaatcag gattcagaag gagatcaatg tcaaatgcgg acaggaacat
     5821 gaaagacgcc tgttatgcgc aattaaaaat ttgggtttaa ttgctgtgga aactgttgtt
     5881 ggcggcatct taagttcctg tttaacaaca tcaactactt atgtacgtag aagcgtttaa
     5941 gccatttgca tacagatgag aactggcttt tgtgctaatc agtcaagatg actccgatga
     6001 tgatgactca ttacctgacc agttttcgct gctttctttt caacaactac ttgtatatgt
     6061 attgtatcca atagcaatac attgaatttc catggtctag tcacgtatta tcatttaatt
     6121 gacaccaagt cgtgttattg ttgagctatc gagttcagct caaacatttc ttattcccat
     6181 gaataagccg gcaaaaatat gcaatctatg aaagttaata taagcaaacc ttactttgac
     6241 tcaataccaa tgcactttgt gtcgataggt tcacgcaatt gaggcgatta ttccgataac
     6301 ccaagcgatt gactgttccc gtttcgattc caattgaaat ttggaaatgt acaatagttt
     6361 tgctatatgc tgtcaagtac gctcttatct tctctgggtt ttcttcagag tttcgaaacg
     6421 cttcttcttt tttttgtttt tttttttttg gaatctcgta ttttggaagg ggctcccctc
     6481 tggaatttgt tacactgtcg ttatcattgc gaacaagcgg cccgaagcta tcagcgactt
     6541 taacatttac aatgcacttt tttacgacca attaaatgta cattttcctt tcttcgcccg
     6601 ttgataagcg aacgcgatgt ggcgcaggca atgtgttgct cttgcgacac aaacgcaatc
     6661 aaaatggatt caatttcgct ttttcccagt gaaacgaaga acgaaccgac catcatgata
     6721 tgctcctctg catgttgcgt attgaatcaa tgacaatttc aattaagccg cccgttcgtc
     6781 atgcgttttc gtgcgcttcg aaatgctgat aacgctgctg tcctccaact gctttgcatg
     6841 tggacacaat tccatttatt taattctttt atttggatcg gttaaattaa aaagcgcctt
     6901 gttacgcatt taacgttgtt tccggtgcgt ggtggtttca tgcttctggg aacggcaaat
     6961 gggtttagga ttgggaaccc ctcatcatct gttggaatat actattcaac ctacaaaagt
     7021 aacgttaaac aacactactt tatatttgat atgaatggcc acacctttta tgccataaaa
     7081 catattgtaa gagaatacca ctctttttat tccttctttc cttcttgtac gttttttgct
     7141 gtaagtaggt cgtggtgctg gtgttgcagt tgaaataact taaaatataa atcataaaac
     7201 tcaaacataa acttgactat ttatttattt attaagaaag gaaatataaa ttataaatta
     7261 caacaggtta tggacctgca gccaagcttg gcgctcgtcc gggggcaatg agatatgaaa
     7321 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     7381 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     7441 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     7501 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     7561 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     7621 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     7681 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     7741 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     7801 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     7861 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     7921 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     7981 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     8041 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     8101 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     8161 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     8221 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     8281 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     8341 agtagatgcc gaccgaacaa gagctgattt cgagaacgcc tcagccagca actcgcgcga
     8401 gcctagcaag gcaaatgcga gagaacggcc ttacgcttgg tggcacagtt ctcgtccaca
     8461 gttcgctaag ctcgctcggc tgggtcgcgg gagggccggt cgcagtgatt caggcccttc
     8521 tggattgtgt tggtccccag ggcacgattg tcatgcccac gcactcgggt gatctgactg
     8581 atcccgcaga ttggagatcg ccgcccgtgc ctgccgattg ggtgcagatc tttgtgaagg
     8641 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     8701 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     8761 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     8821 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     8881 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     8941 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     9001 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     9061 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
     9121 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
     9181 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
     9241 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     9301 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     9361 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9421 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatc
//
