LOCUS       Exported               10400 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Repression of gene expression in Streptomyces by CRISPRi.
ACCESSION   .
VERSION     .
KEYWORDS    pSET-dCas9-actII-4-NT-S1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   Zhao Y, Li L, Zheng G, Jiang W, Deng Z, Wang Z, Lu Y
  TITLE     CRISPR/dCas9-Mediated Multiplex Gene Repression in Streptomyces.
  JOURNAL   Biotechnol J. 2018 Jun 3. doi: 10.1002/biot.201800121.
  PUBMED    29862648
REFERENCE   2  (bases 1 to 10400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10400
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     408..430
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     422..439
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     423..439
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             666..4772
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        4954..4988
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        5009..5084
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      5101..5135
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(5193..5209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5193..5209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5206..5228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5217..5233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5241..5271)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5286..5307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5424..5441)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5595..6183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5675..5694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             6387..7187
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             complement(7621..7992)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            8025..8134
                     /note="incP origin of transfer"
     CDS             complement(8372..8383)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    8454..8553
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             8563..10380
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
ORIGIN
        1 atctacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
       61 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
      121 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
      181 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta tgcggtgtga
      241 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
      301 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      361 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      421 ttgtaaaacg acggccagtg ccaagcttgg gctgcaggtc gactctagac tctagtatgc
      481 atgcgagtgt ccgttcgagt ggcggcttgc gcccgatgct agtcgcggtt gatcggcgat
      541 cgcaggtgca cgcggtcgat cttgacggct ggcgagaggt gcggggagga tctgaccgac
      601 gcggtccaca cgtggcaccg cgatgctgtt gtgggcacaa tcgtgccggt tggtaggatc
      661 cacatatgga caagaagtac tccatcggcc tggcgatcgg caccaactcc gtcggctggg
      721 ccgtcatcac ggacgagtac aaggtcccgt cgaagaagtt caaggtgctg ggcaacacgg
      781 accgccacag catcaagaag aacctcatcg gcgccctcct gttcgacagc ggcgagacgg
      841 ccgaggcgac ccgcctgaag cgcacggcgc ggcggcggta cacgcgccgc aagaaccgca
      901 tctgctacct ccaggagatc ttctccaacg agatggcgaa ggtcgacgac tcgttcttcc
      961 accggctgga ggagtccttc ctggtcgagg aggacaagaa gcacgagcgg caccccatct
     1021 tcggcaacat cgtggacgag gtggcctacc acgagaagta cccgaccatc taccacctcc
     1081 ggaagaagct ggtggactcg accgacaagg ccgacctgcg cctgatctac ctggccctcg
     1141 cccacatgat caagttccgc ggccacttcc tcatcgaggg cgacctgaac ccggacaact
     1201 cggacgtcga caagctgttc atccagctcg tgcagaccta caaccagctc ttcgaggaga
     1261 acccgatcaa cgcctccggc gtggacgcga aggccatcct gtcggcccgc ctctcgaagt
     1321 cccggcggct cgagaacctg atcgcccagc tgccgggcga gaagaagaac ggcctcttcg
     1381 gcaacctgat cgcgctctcc ctgggcctga cgccgaactt caagtccaac ttcgacctcg
     1441 ccgaggacgc caagctccag ctgtccaagg acacgtacga cgacgacctg gacaacctcc
     1501 tggcgcagat cggcgaccag tacgcggacc tgttcctcgc ggcgaagaac ctctccgacg
     1561 cgatcctgct cagcgacatc ctccgcgtca acaccgagat caccaaggcg cccctgtccg
     1621 cgagcatgat caagcgctac gacgagcacc accaggacct caccctgctg aaggccctgg
     1681 tccgccagca gctcccggag aagtacaagg agatcttctt cgaccagtcc aagaacggct
     1741 acgcgggcta catcgacggc ggcgcgtccc aggaggagtt ctacaagttc atcaagccca
     1801 tcctggagaa gatggacggc accgaggagc tgctggtcaa gctgaaccgg gaggacctcc
     1861 tgcgcaagca gcgcacgttc gacaacggct ccatccccca ccagatccac ctgggcgagc
     1921 tgcacgccat cctccggcgc caggaggact tctacccgtt cctcaaggac aaccgcgaga
     1981 agatcgagaa gatcctcacg ttccggatcc cgtactacgt cggccccctc gcccgcggca
     2041 actcccgctt cgcgtggatg acgcgcaagt cggaggagac gatcaccccg tggaacttcg
     2101 aggaggtggt ggacaagggc gcctccgcgc agtcgttcat cgagcggatg accaacttcg
     2161 acaagaacct gccgaacgag aaggtgctgc ccaagcacag cctcctgtac gagtacttca
     2221 cggtctacaa cgagctcacc aaggtgaagt acgtgacgga gggcatgcgg aagccggcgt
     2281 tcctgtccgg cgagcagaag aaggccatcg tggacctgct gttcaagacg aaccggaagg
     2341 tcaccgtcaa gcagctgaag gaggactact tcaagaagat cgagtgcttc gactcggtcg
     2401 agatcagcgg cgtggaggac cgcttcaacg cgtcgctggg cacgtaccac gacctcctga
     2461 agatcatcaa ggacaaggac ttcctcgaca acgaggagaa cgaggacatc ctggaggaca
     2521 tcgtgctgac cctgacgctc ttcgaggacc gggagatgat cgaggagcgc ctcaagacct
     2581 acgcgcacct gttcgacgac aaggtcatga agcagctcaa gcggcggcgg tacaccggct
     2641 ggggccggct ctcccgcaag ctcatcaacg gcatccggga caagcagtcg ggcaagacca
     2701 tcctcgactt cctgaagtcc gacggcttcg ccaaccgcaa cttcatgcag ctgatccacg
     2761 acgacagcct gacgttcaag gaggacatcc agaaggcgca ggtgtcgggc cagggcgact
     2821 ccctgcacga gcacatcgcg aacctggccg gcagcccggc catcaagaag ggcatcctgc
     2881 agacggtcaa ggtggtcgac gagctggtga aggtgatggg ccgccacaag cccgagaaca
     2941 tcgtcatcga gatggcccgg gagaaccaga ccacgcagaa gggccagaag aacagccgcg
     3001 agcgcatgaa gcggatcgag gagggcatca aggagctcgg ctcgcagatc ctcaaggagc
     3061 accccgtcga gaacacccag ctccagaacg agaagctcta cctctactac ctgcagaacg
     3121 gccgggacat gtacgtggac caggagctcg acatcaaccg gctgtcggac tacgacgtgg
     3181 acgccatcgt cccgcagtcg ttcctgaagg acgacagcat cgacaacaag gtcctcaccc
     3241 gctcggacaa gaaccggggc aagtccgaca acgtcccgtc ggaggaggtc gtgaagaaga
     3301 tgaagaacta ctggcggcag ctcctcaacg ccaagctcat cacgcagcgc aagttcgaca
     3361 acctgacgaa ggcggagcgg ggcggcctca gcgagctcga caaggcgggc ttcatcaagc
     3421 ggcagctggt cgagacgcgg cagatcacga agcacgtggc ccagatcctg gactcgcgca
     3481 tgaacaccaa gtacgacgag aacgacaagc tgatccgcga ggtcaaggtc atcaccctca
     3541 agtccaagct ggtgagcgac ttccgcaagg acttccagtt ctacaaggtg cgggagatca
     3601 acaactacca ccacgcccac gacgcgtacc tgaacgcggt ggtgggcacg gcgctgatca
     3661 agaagtaccc gaagctcgag agcgagttcg tctacggcga ctacaaggtg tacgacgtgc
     3721 ggaagatgat cgcgaagagc gagcaggaga tcggcaaggc cacggcgaag tacttcttct
     3781 acagcaacat catgaacttc ttcaagaccg agatcaccct ggcgaacggc gagatccgca
     3841 agcgcccgct catcgagacc aacggcgaga cgggcgagat cgtgtgggac aagggccgcg
     3901 acttcgcgac cgtccggaag gtgctgtcca tgccccaggt caacatcgtc aagaagacgg
     3961 aggtccagac cggcggcttc agcaaggagt ccatcctgcc caagcggaac agcgacaagc
     4021 tgatcgcccg gaagaaggac tgggacccca agaagtacgg cggcttcgac tcccccaccg
     4081 tcgcctactc ggtcctcgtg gtcgccaagg tggagaaggg caagagcaag aagctgaagt
     4141 ccgtgaagga gctgctgggc atcaccatca tggagcggtc gtcgttcgag aagaacccca
     4201 tcgacttcct ggaggccaag ggctacaagg aggtgaagaa ggacctcatc atcaagctcc
     4261 ccaagtactc cctgttcgag ctggagaacg gccggaagcg catgctcgcc agcgcgggcg
     4321 agctgcagaa gggcaacgag ctggcgctgc cgagcaagta cgtcaacttc ctgtacctcg
     4381 cctcgcacta cgagaagctg aagggctcgc cggaggacaa cgagcagaag cagctgttcg
     4441 tggagcagca caagcactac ctcgacgaga tcatcgagca gatctccgag ttctccaagc
     4501 gcgtcatcct ggccgacgcg aacctggaca aggtcctgtc cgcgtacaac aagcaccgcg
     4561 acaagccgat ccgggagcag gccgagaaca tcatccacct gttcacgctg accaacctcg
     4621 gcgcccccgc ggccttcaag tacttcgaca ccacgatcga ccggaagcgg tacacgagca
     4681 cgaaggaggt gctcgacgcc accctgatcc accagagcat cacgggcctg tacgagaccc
     4741 gcatcgacct ctcccagctg ggcggcgact gaatttaaat atctaaagtt ttgtcgtctt
     4801 tccagacgtt agtaaatgaa ttttctgtat gaggttttgc taaacaactt tcaacagttt
     4861 cagcggagtg agaatagaaa ggaacaacta aaggaattgc gaataataat tttttcacgt
     4921 tgaaaatctc gatatctcaa gggtaccgga tccttgacag ctagctcagt cctaggtata
     4981 atactagtgt cgccttcgag gatttaaggt tttagagcta gaaatagcaa gttaaaataa
     5041 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tgagtcacca
     5101 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     5161 attactggat gtacacccga attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt
     5221 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5281 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5341 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5401 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5461 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5521 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5581 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5641 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5701 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5761 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5821 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5881 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5941 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6001 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6061 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6121 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6181 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6241 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttggttcat
     6301 gtgcagctcc atcagcaaaa ggggatgata agtttatcac caccgactat ttgcaacagt
     6361 gccgttgatc gtgctatgat cgactgatgt catcagcggt ggagtgcaat gtcgtgcaat
     6421 acgaatggcg aaaagccgag ctcatcggtc agcttctcaa ccttggggtt acccccggcg
     6481 gtgtgctgct ggtccacagc tccttccgta gcgtccggcc cctcgaagat gggccacttg
     6541 gactgatcga ggccctgcgt gctgcgctgg gtccgggagg gacgctcgtc atgccctcgt
     6601 ggtcaggtct ggacgacgag ccgttcgatc ctgccacgtc gcccgttaca ccggaccttg
     6661 gagttgtctc tgacacattc tggcgcctgc caaatgtaaa gcgcagcgcc catccatttg
     6721 cctttgcggc agcggggcca caggcagagc agatcatctc tgatccattg cccctgccac
     6781 ctcactcgcc tgcaagcccg gtcgcccgtg tccatgaact cgatgggcag gtacttctcc
     6841 tcggcgtggg acacgatgcc aacacgacgc tgcatcttgc cgagttgatg gcaaaggttc
     6901 cctatggggt gccgagacac tgcaccattc ttcaggatgg caagttggta cgcgtcgatt
     6961 atctcgagaa tgaccactgc tgtgagcgct ttgccttggc ggacaggtgg ctcaaggaga
     7021 agagccttca gaaggaaggt ccagtcggtc atgcctttgc tcggttgatc cgctcccgcg
     7081 acattgtggc gacagccctg ggtcaactgg gccgagatcc gttgatcttc ctgcatccgc
     7141 cagaggcggg atgcgaagaa tgcgatgccg ctcgccagtc gattggctga gctcatgagc
     7201 ggagaacgag atgacgttgg aggggcaagg tcgcgctgat tgctggggca acacgtggag
     7261 cggatcgggg attgtctttc ttcagctcgc tgatgatatg ctgacgctca atgccgtttg
     7321 gcctccgact aacgaaaatc ccgcatttgg acggctgatc cgattggcac ggcggacggc
     7381 gaatggcgga gcagacgctc gtccgggggc aatgagatat gaaaaagcct gaactcaccg
     7441 cgacgtatcg ggccctggcc agctagctag agtcgacctg caggtccccg gggatcggtc
     7501 ttgccttgct cgtcggtgat gtacttcacc agctccgcga agtcgctctt cttgatggag
     7561 cgcatgggga cgtgcttggc aatcacgcgc accccccggc cgttttagcg gctaaaaaag
     7621 tcatggctct gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt
     7681 ctcttcgatc ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg
     7741 gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg
     7801 ggccagctcg cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac
     7861 ggccagcagg taggccgaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct
     7921 tcgttcgtct ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt
     7981 ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag
     8041 agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc
     8101 gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg
     8161 aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac
     8221 cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaaga tccgtcgacc
     8281 tgcaggcatg caagctctag cgattccaga cgtcccgaag gcgtggcgcg gcttccccgt
     8341 gccggagcaa tcgccctggg tgggttacac gacgcccctc tatggcccgt actgacggac
     8401 acaccgaagc cccggcggca accctcagcg gatgccccgg ggcttcacgt tttcccaggt
     8461 cagaagcggt tttcgggagt agtgccccaa ctggggtaac ctttgagttc tctcagttgg
     8521 gggcgtaggg tcgccgacat gacacaaggg gttgtgaccg gggtggacac gtacgcgggt
     8581 gcttacgacc gtcagtcgcg cgagcgcgaa aattcgagcg cagcaagccc agcgacacag
     8641 cgtagcgcca acgaagacaa ggcggccgac cttcagcgcg aagtcgagcg cgacgggggc
     8701 cggttcaggt tcgtcgggca tttcagcgaa gcgccgggca cgtcggcgtt cgggacggcg
     8761 gagcgcccgg agttcgaacg catcctgaac gaatgccgcg ccgggcggct caacatgatc
     8821 attgtctatg acgtgtcgcg cttctcgcgc ctgaaggtca tggacgcgat tccgattgtc
     8881 tcggaattgc tcgccctggg cgtgacgatt gtttccactc aggaaggcgt cttccggcag
     8941 ggaaacgtca tggacctgat tcacctgatt atgcggctcg acgcgtcgca caaagaatct
     9001 tcgctgaagt cggcgaagat tctcgacacg aagaaccttc agcgcgaatt gggcgggtac
     9061 gtcggcggga aggcgcctta cggcttcgag cttgtttcgg agacgaagga gatcacgcgc
     9121 aacggccgaa tggtcaatgt cgtcatcaac aagcttgcgc actcgaccac tccccttacc
     9181 ggacccttcg agttcgagcc cgacgtaatc cggtggtggt ggcgtgagat caagacgcac
     9241 aaacaccttc ccttcaagcc gggcagtcaa gccgccattc acccgggcag catcacgggg
     9301 ctttgtaagc gcatggacgc tgacgccgtg ccgacccggg gcgagacgat tgggaagaag
     9361 accgcttcaa gcgcctggga cccggcaacc gttatgcgaa tccttcggga cccgcgtatt
     9421 gcgggcttcg ccgctgaggt gatctacaag aagaagccgg acggcacgcc gaccacgaag
     9481 attgagggtt accgcattca gcgcgacccg atcacgctcc ggccggtcga gcttgattgc
     9541 ggaccgatca tcgagcccgc tgagtggtat gagcttcagg cgtggttgga cggcaggggg
     9601 cgcggcaagg ggctttcccg ggggcaagcc attctgtccg ccatggacaa gctgtactgc
     9661 gagtgtggcg ccgtcatgac ttcgaagcgc ggggaagaat cgatcaagga ctcttaccgc
     9721 tgccgtcgcc ggaaggtggt cgacccgtcc gcacctgggc agcacgaagg cacgtgcaac
     9781 gtcagcatgg cggcactcga caagttcgtt gcggaacgca tcttcaacaa gatcaggcac
     9841 gccgaaggcg acgaagagac gttggcgctt ctgtgggaag ccgcccgacg cttcggcaag
     9901 ctcactgagg cgcctgagaa gagcggcgaa cgggcgaacc ttgttgcgga gcgcgccgac
     9961 gccctgaacg cccttgaaga gctgtacgaa gaccgcgcgg caggcgcgta cgacggaccc
    10021 gttggcagga agcacttccg gaagcaacag gcagcgctga cgctccggca gcaaggggcg
    10081 gaagagcggc ttgccgaact tgaagccgcc gaagccccga agcttcccct tgaccaatgg
    10141 ttccccgaag acgccgacgc tgacccgacc ggccctaagt cgtggtgggg gcgcgcgtca
    10201 gtagacgaca agcgcgtgtt cgtcgggctc ttcgtagaca agatcgttgt cacgaagtcg
    10261 actacgggca gggggcaggg aacgcccatc gagaagcgcg cttcgatcac gtgggcgaag
    10321 ccgccgaccg acgacgacga agacgacgcc caggacggca cggaagacgt agcggcgtag
    10381 cgagacaccc gggaagcctg
//
