LOCUS       Exported                1881 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  Cation channelrhodopsin ChR2 fused to mRuby2 fluorophore and 
            targeted to the neuronal soma and proximal dendrites.
ACCESSION   .
VERSION     .
KEYWORDS    pCAG-ChR2-mRuby2-ST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Mardinly AR, Oldenburg IA, Pegard NC, Sridharan S, Lyall EH, Chesnov
            K, Brohawn SG, Waller L, Adesnik H
  TITLE     Precise multimodal optical control of neural ensemble activity.
  JOURNAL   Nat Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8.
  PUBMED    29713079
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..927
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             964..1674
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 atggattatg gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt aacgaaccca
       61 gtagtcgtca atggctctgt acttgtgcct gaggaccagt gttactgcgc gggctggatt
      121 gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg gcttgctgct
      181 ggcttctcca tcctactgct tatgttttac gcctaccaaa catggaagtc aacctgcggc
      241 tgggaggaga tctatgtgtg cgctatcgag atggtcaagg tgattctcga gttcttcttc
      301 gagtttaaga acccgtccat gctgtatcta gccacaggcc accgcgtcca gtggttgcgt
      361 tacgccgagt ggcttctcac ctgcccggtc attctcattc acctgtcaaa cctgacgggc
      421 ttgtccaacg actacagcag gcgcaccatg ggtctgcttg tgtctgatat tggcacaatt
      481 gtgtggggcg ccacttccgc catggccacc ggatacgtca aggtcatctt cttctgcctg
      541 ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca aggcctacat cgagggttac
      601 cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg gctcttcttc
      661 gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg
      721 agcgtgtacg gctccaccgt cggccacacc atcattgacc tgatgtcgaa gaactgctgg
      781 ggtctgctcg gccactacct gcgcgtgctg atccacgagc atatcctcat ccacggcgac
      841 attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga gacgctggtg
      901 gaggacgagg ccgaggctgg cgcggtcaac aagggcaccg gcaagccacc ggtcgcaccg
      961 gtaatggtgt ctaagggcga agagctgatc aaggaaaata tgcgtatgaa ggtggtcatg
     1021 gaaggttcgg tcaacggcca ccaattcaaa tgcacaggtg aaggagaagg caatccgtac
     1081 atgggaactc aaaccatgag gatcaaagtc atcgagggag gacccctgcc atttgccttt
     1141 gacattcttg ccacgtcgtt catgtatggc agccgtactt ttatcaagta cccgaaaggc
     1201 attcctgatt tctttaaaca gtcctttcct gagggtttta cttgggaaag agttacgaga
     1261 tacgaagatg gtggagtcgt caccgtcatg caggacacca gccttgagga tggctgtctc
     1321 gtttaccacg tccaagtcag aggggtaaac tttccctcca atggtcccgt gatgcagaag
     1381 aagaccaagg gttgggagcc taatacagag atgatgtatc cagcagatgg tggtctgagg
     1441 ggatacactc atatggcact gaaagttgat ggtggtggcc atctgtcttg ctctttcgta
     1501 acaacttaca ggtcaaaaaa gaccgtcggg aacatcaaga tgcccggtat ccatgccgtt
     1561 gatcaccgcc tggaaaggtt agaggaaagt gacaatgaaa tgttcgtagt acaacgcgaa
     1621 cacgcagttg ccaagttcgc cgggcttggt ggtgggatgg acgagctgta caaggcccta
     1681 gagcagtccc agcccatcct caacaccaag gagatggccc cgcagagcaa gcctccagag
     1741 gagctggaga tgagcagcat gcccagcccc gtggcccctc tgcccgcacg cacggagggc
     1801 gtcatcgaca tgcggagcat gtccagcatt gacagcttca tcagctgtgc cacggacttc
     1861 cctgaagcca ccagattcta a
//
