LOCUS Exported 9985 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Fibroblasts specific inducible Cre. ACCESSION . VERSION . KEYWORDS Col1A2:TFPnls-T2a-mErt-Cre-Ert SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9985) TITLE Cre-inducible plasmids REFERENCE 2 (bases 1 to 9985) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9985 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(2..457) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(139..160) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 351..370 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 584..606 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 598..615 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 599..615 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 648..667 /label=T7 /note="T7 promoter, forward primer" promoter 648..666 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2452..2468) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2453..2469) /label=pBluescriptKS /note="For pBluescript vector" intron 2940..3072 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 3055..3074 /label=pMT2-F /note="Synthetic intron, forward primer" primer_bind 3169..3188 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" regulatory 3236..3245 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3242..3949 /codon_start=1 /product="monomeric teal (cyan) fluorescent protein (Ai et al., 2006)" /label=mTFP1 /note="mammalian codon-optimized" /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA VARNSTDGMDELYK" CDS 3974..3994 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 3998..4018 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4022..4042 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4064..4117 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 5072..6094 /codon_start=1 /product="site-specific recombinase" /label=Cre /note="Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences." /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG D" CDS 6107..7042 /codon_start=1 /product="mutated ligand-binding domain of the human estrogen receptor (Feil et al., 1997)" /label=ERT2 /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET DQSHLATAGSTSSHSLQKYYITGEAEGFPAT" primer_bind complement(7132..7151) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 7197..7252 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(7251..7270) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(7773..7793) /label=T3 /note="T3 promoter, forward primer" promoter complement(7773..7791) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(7835..7851) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7835..7851) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7848..7870) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7859..7875 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7883..7913) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7928..7949 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8066..8083) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8237..8825) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8317..8336) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8996..9856) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9619..9638 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9857..9961) /gene="bla" /label=AmpR promoter ORIGIN 1 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 61 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 121 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 181 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 241 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 301 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 361 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 421 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc 481 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg 541 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt 601 aaaacgacgg ccagtgagcg cgtagggata acagggtaat gcgcgcgtaa tacgactcac 661 tatagggcga attgggtacc cagaggtgta aagtacttga gtaattttac ttgattactg 721 tacttaagta ttatttttgg ggatttttac tttacttgag tacaattaaa aatcaatact 781 tttactttta cttaattaca tttttttaga aaaaaaagta ctttttactc cttacaattt 841 tatttacagt caaaaagtac ttattttttg gagatcactt gggcccggct cgagcatgca 901 cgcgtgaatt ccccaatttt tctctgtgaa tctcctccca cacacacacc ctttttctcc 961 tgggcttcag catccatcgt aactagttag ctccactcac attatccact attttactca 1021 aaatgcgagg attactcttt gctttatctt caagagaagt ggagagagcc agacaaagga 1081 agaagaggga gaacaggaga ggagatgagg ggggagatta gaatggggta agggagagaa 1141 caggagctga gaagagcggg agagggggag agaaggattc agacattcta aactctcttc 1201 ctgagtgcta tttaaagcag tttccaacct ggagagaatg taatgcacag ttcctccatg 1261 tgtttagtta aggacctcat gagtcttgtg gatttctcca caacctaagt ggttattatg 1321 ctgacccgag ggtgtgccat ttggtgggac acttcatctt attgtccctg tttatgtgta 1381 gcaagcaagt tcagttctgt aagacactga ttagaggggc aggaaaattg ttctctactg 1441 tgccttgtgt acagcaagaa gctgtccagt gttgttcaca taattgaatg tgtggaactt 1501 cccagcttgc aggtggggtc ttcagttcta cctcagtctc tttgggagac taccacaact 1561 caccttgagt aattagaaaa cagcacagaa gtggctgcgc ttcctgaaga ctacaaagga 1621 gctgttgcct gcttctctta actccatctt gggctcagct gacccaaaag cggctgtctt 1681 tgtttacacc agatttcaac tttgcaggca atcttactaa gcagagggag gcagtggggg 1741 atggtattgg gggtgagtta cttagtccaa gtcccagagc tgagggtgag ggagatgctg 1801 tactttctta ccagctgtta tgcatcaaga agaggcaggc ttgtgtaacc ctttcttctc 1861 ctgcacgttt caagcaaaag ccaggcactc actgagtttt gtaaagattt tcccgtgaag 1921 acatacacac agcgtaattc catgcaataa tttctgtgta aagcccttag tgcagtgtac 1981 cattgtttgt gtctgggtct tgaattgtca caattaagtg tgagcacaca cacaatgtgc 2041 tctttagcca aagtcataac actgctacct gcttcccttt gaaagaaaac agccatcttc 2101 tcaggaaatg taagggggca aggggtgggg gttaggggga aggacaagat tgtaaaaagc 2161 caaaagccta gggcttggag ctgctttggc caagcctaga atctatttaa atgcccttcc 2221 ctgaagggtt aagtgtggaa cacctcccag gaaatgacaa atggccagag ttctgcccac 2281 tcccttcccc ccatcttcca gacatcaaat acttaggaac tgtttggctt gaaatggttc 2341 ctgagactca tactcacaga acacattctt tttactctcc tcccccagcc cagtacaggc 2401 tggaggtggc tggcgggggt agggggggat gggtgaaggg ggcaagctta tcgataccgt 2461 cgacctcgag gttctagagc ttggatctgt aaagagccca cgtaggtgtc ctaaagtgct 2521 tccaaacttg gcaagggcga gagagggcgg gtggctgggg agggcggagg tatgcagaca 2581 gggagtcaga gttccccctc gaaagcctca aaagtgtcca cgtcctcaaa aagaatggaa 2641 ccaatttaag aagccccgta gccacgtccc tcccccctcg gctccccccc ctgctccccc 2701 gcagtctcct cccagcactg agtcccgggc ccctagccct agccctccca ttggtggaga 2761 cgtttttgga ggcaccctcc ggctggggaa acttttccca tataaataag gcaggtctgg 2821 gctttattat tttagcacca cggcagcagg aggtttcgac taagttggag ggaacggtcc 2881 acgattgcca aggaattcga attttaagct gcagaagttg gtcgtgaggc actgggcagg 2941 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac 3001 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct 3061 ttctctccac aggtgtccac tcccagttca attacagctc ttaaaattcc gccactagag 3121 cctctgctaa ccatgttcat gccttcttct ctttcctaca gctcctgggc aacgtgctgg 3181 ttattgtgct gtctcatcat tttggcaaag aattattccg ctagcgctac cggtcgccac 3241 catggtgagc aagggcgagg agaccacaat gggcgtaatc aagcccgaca tgaagatcaa 3301 gctgaagatg gagggcaacg tgaatggcca cgccttcgtg atcgagggcg agggcgaggg 3361 caagccctac gacggcacca acaccatcaa cctggaggtg aaggagggag cccccctgcc 3421 cttctcctac gacattctga ccaccgcgtt cgcctacggc aacagggcct tcaccaagta 3481 ccccgacgac atccccaact acttcaagca gtccttcccc gagggctact cttgggagcg 3541 caccatgacc ttcgaggaca agggcatcgt gaaggtgaag tccgacatct ccatggagga 3601 ggactccttc atctacgaga tacacctcaa gggcgagaac ttccccccca acggccccgt 3661 gatgcagaag aagaccaccg gctgggacgc ctccaccgag aggatgtacg tgcgcgacgg 3721 cgtgctgaag ggcgacgtca agcacaagct gctgctggag ggcggcggcc accaccgcgt 3781 tgacttcaag accatctaca gggccaagaa ggcggtgaag ctgcccgact atcactttgt 3841 ggaccaccgc atcgagatcc tgaaccacga caaggactac aacaaggtga ccgtttacga 3901 gagcgccgtg gcccgcaatt ccaccgacgg catggacgag ctgtacaagt ccggactcag 3961 atctcgagct gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga gaaaggtaga 4021 tccaaaaaag aagagaaagg taggatccac cggatctaga cccgagggca gaggaagtct 4081 tctaacatgc ggtgacgtgg aggagaatcc cggcccacta gtcatggccg gcgatatgag 4141 agccgctaac ctgtggccct ccccactgat gatcaagaga agcaagaaga actccctggc 4201 cctgagcctg accgctgatc agatggtgtc cgccctgctg gacgccgagc cccctatcct 4261 gtacagcgag tacgacccca ccaggccctt cagcgaggcc agcatgatgg gactgctgac 4321 aaacctggcc gatcgcgaac tggtgcatat gatcaattgg gccaagcgcg tgcccggatt 4381 cgtggacctg acactgcacg accaggtgca cctgctggaa tgcgcttggc tggaaattct 4441 gatgatcggc ctcgtgtggc ggagcatgga acatcctgtg aagctgctgt tcgcccccaa 4501 tctgctgctg gaccgcaacc agggcaaatg cgtggaaggg atggtcgaaa tctttgatat 4561 gctgctcgcc accagcagca ggttcagaat gatgaacctc cagggcgaag agttcgtctg 4621 cctgaagtcc atcatcctgc tgaacagcgg cgtgtacacc ttcctgtcct ctaccctgaa 4681 gtccctcgag gaaaaggatc acatccacag ggtgctggat aagatcaccg ataccctgat 4741 ccatctcatg gctaaggccg gactcacact ccagcagcag catcagagac tcgctcagct 4801 gctgctgatc ctgagccaca tccgccacat gtccaacaag ggaatggaac acctctactc 4861 tatgaagtgt aaaaacgtcg tccccctgta cgatctgctg ctcgaggccg ccgatgccca 4921 cagactgcac gcccctacaa gcagaggcgg cgctagcgtg gaagagacag accagtccca 4981 cctggccacc gccggcagca caagcagcca cagcctgcag aagtactata tcaccggcga 5041 ggccgagggc ttccccgcta cagctgtcga caatttactg accgtacacc aaaatttgcc 5101 tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag 5161 ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg 5221 ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg 5281 cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt 5341 gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc 5401 tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa 5461 acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag 5521 cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt 5581 acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag 5641 aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc 5701 acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga 5761 tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc 5821 caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat 5881 ttacggcgct aaggatgact ccggtcagag atacctggcc tggtctggac acagtgcccg 5941 tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc 6001 tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac 6061 aggggcaatg gtgcgcctgc tggaagatgg cgatctcgag ccatctgctg gagacatgag 6121 agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga acagcctggc 6181 cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc cccccatact 6241 ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg gcttactgac 6301 caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg tgccaggctt 6361 tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc tagagatcct 6421 gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt ttgctcctaa 6481 cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga tcttcgacat 6541 gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg agtttgtgtg 6601 cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca gcaccctgaa 6661 gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag acactttgat 6721 ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc tggcccagct 6781 cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc atctgtacag 6841 catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg cggacgccca 6901 ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg accaaagcca 6961 cttggccact gcgggctcta cttcatcgca ttccttgcaa aagtattaca tcacggggga 7021 ggcagagggt ttccctgcca cagcttaaga tatcactcct caggtgcagg ctgcctatca 7081 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc 7141 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata 7201 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag 7261 gacatatggg agggcaaatc atttaaaaca tcagaatgag catttggttt agagtttggc 7321 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta 7381 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta 7441 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt 7501 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc 7561 tcttctctta tgaagatccc tcgaccagcg gccgctaata ctcaagtaca attttaatgg 7621 agtacttttt tacttttact caagtaagat tctagccaga tacttttact tttaattgag 7681 taaaattttc cctaagtact tgtactttca cttgagtaaa atttttgagt actttttaca 7741 cctctgccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcatt 7801 accctgttat ccctacgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 7861 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 7921 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 7981 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 8041 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 8101 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 8161 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 8221 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 8281 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 8341 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 8401 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 8461 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 8521 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 8581 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 8641 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 8701 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 8761 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 8821 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 8881 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 8941 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 9001 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 9061 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 9121 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 9181 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 9241 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 9301 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 9361 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 9421 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 9481 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 9541 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 9601 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 9661 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 9721 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 9781 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 9841 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 9901 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 9961 gcacatttcc ccgaaaagtg ccacc //