LOCUS       Exported                 986 bp ds-DNA     linear   SYN 10-JUL-2021
DEFINITION  Expresses channel rhodopsin in astrocytes.
ACCESSION   .
VERSION     .
KEYWORDS    pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Octeau JC, Gangwani MR, Allam SL, Tran D, Huang S, Hoang-Trong TM, 
            Golshani P, Rumbell TH, Kozloski JR, Khakh BS
  TITLE     Transient, Consequential Increases in Extracellular Potassium Ions 
            Accompany Channelrhodopsin2 Excitation.
  JOURNAL   Cell Rep. 2019 May 21;27(8):2249-2261.e7. doi: 
            10.1016/j.celrep.2019.04.078.
  PUBMED    31116972
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 10, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     intron          100..232
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     215..234
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        277..295
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      303..312
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 gnggnanttt atcacagtta aattgctaac gcagtcagtg cttctgacac aacagtctcg
       61 aacttaagct gcagaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga
      121 caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc
      181 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac
      241 tcccagttca attacagctc ttaaggctag agtacttaat acgactcact ataggctagc
      301 ccgccaccat ggactatggc ggcgctttgt ctgccgtcgg acgcgaactt ttgttcgtta
      361 ctaatcctgt ggtggtgaac gggtccgtcc tggtccctga ggatcaatgt tactgtgccg
      421 gatggattga atctcgcggc acgaacggcg ctcagaccgc gtcaaatgtc ctgcagtggc
      481 ttgcagcagg attcagcatt ttgctgctga tgttctatgc ctaccaaacc tggaaatcta
      541 catgcggctg ggaggagatc tatgtgtgcg ccattgaaat ggttaaggtg attctcgagt
      601 tcttttttga gtttaagaat ccctctatgc tctaccttgc cacaggacac cgggtgcagt
      661 ggctgcgcta tgcagagtgg ctgctcactt gtcctgtcat ccttatccgc ctgagcaacc
      721 tcaccggcct gagcaacgac tacagcagga gaaccatggg actccttgtc tcagacatcg
      781 ggactatcgt gtggggggct accagcgcca tggcaaccgg ctatgttaaa gtcatcttct
      841 tttgtcttgg attgtgctat ggcgcgaaca cattttttca cgccgccaaa gcatatatcg
      901 agggttatca tactgtgcca aagggtcggt gccgccaggt cgtgaccggc atggnatnnt
      961 gttttcgtga gctggggtat gttccc
//
