LOCUS       Exported                 983 bp ds-DNA     linear   SYN 11-JUL-2021
DEFINITION  Expresses channel rhodopsin in astrocytes.
ACCESSION   .
VERSION     .
KEYWORDS    pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Octeau JC, Gangwani MR, Allam SL, Tran D, Huang S, Hoang-Trong TM, 
            Golshani P, Rumbell TH, Kozloski JR, Khakh BS
  TITLE     Transient, Consequential Increases in Extracellular Potassium Ions 
            Accompany Channelrhodopsin2 Excitation.
  JOURNAL   Cell Rep. 2019 May 21;27(8):2249-2261.e7. doi: 
            10.1016/j.celrep.2019.04.078.
  PUBMED    31116972
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     14..33
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        76..94
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      102..111
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 actgacancc actttgcctt tctctccaca ggtgtccacn nccagttcaa ttacagctct
       61 taaggctaga gtacttaata cgactcacta taggctagcc cgccaccatg gactatggcg
      121 gcgctttgtc tgccgtcgga cgcgaacttt tgttcgttac taatcctgtg gtggtgaacg
      181 ggtccgtcct ggtccctgag gatcaatgtt actgtgccgg atggattgaa tctcgcggca
      241 cgaacggcgc tcagaccgcg tcaaatgtcc tgcagtggct tgcagcagga ttcagcattt
      301 tgctgctgat gttctatgcc taccaaacct ggaaatctac atgcggctgg gaggagatct
      361 atgtgtgcgc cattgaaatg gttaaggtga ttctcgagtt cttttttgag tttaagaatc
      421 cctctatgct ctaccttgcc acaggacacc gggtgcagtg gctgcgctat gcagagtggc
      481 tgctcacttg tcctgtcatc cttatccgcc tgagcaacct caccggcctg agcaacgact
      541 acagcaggag aaccatggga ctccttgtct cagacatcgg gactatcgtg tggggggcta
      601 ccagcgccat ggcaaccggc tatgttaaag tcatcttctt ttgtcttgga ttgtgctatg
      661 gcgcgaacac attttttcac gccgccaaag catatatcga gggttatcat actgtgccaa
      721 agggtcggtg ccgccaggtc gtgaccggca tggcatggct gtttttcgtg agctggggta
      781 tgttcccaat tctcttcatt ttggggcccg aaggttttgg cgtcctgagc gtctatggct
      841 ccaccgtagg tcacacgatt attgatctga tgagtaaaaa ttgttggggg ttgttgggac
      901 actacctgcg cgtcctgatc cacgagcaca tattgattca cggagatatc cgcaaaacca
      961 ccaaactgaa catcggcnga acg
//
