LOCUS Exported 5998 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses full length PARP1 in Sf9 (insect) cells. ACCESSION . VERSION . KEYWORDS pACEBac1-PARP1-Flag-His-WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5998) AUTHORS Liszczak G, Diehl KL, Dann GP, Muir TW TITLE Acetylation blocks DNA damage-induced chromatin ADP-ribosylation. JOURNAL Nat Chem Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 10.1038/s41589-018-0097-1. PUBMED 30013063 REFERENCE 2 (bases 1 to 5998) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5998 /organism="synthetic DNA construct" /mol_type="other DNA" mobile_element 135..358 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (right end of the Tn7 transposon)" CDS complement(425..958) /codon_start=1 /gene="aacC1" /product="gentamycin acetyltransferase" /label=GmR /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(1147..1175) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" protein_bind 1246..1279 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter 1356..1447 /gene="polh from Autographa californica" /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" primer_bind 1372..1389 /label=Polyhedrin-F /note="Polyhedrin promoter, forward primer" CDS 4547..4570 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 4610..4627 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" polyA_signal 4800..4934 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4837..4856) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4891..4910 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" mobile_element 5055..5220 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" rep_origin 5410..5998 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5899..5918 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 61 ttctttcctg cgttatcccc tgattgactt gggtcgctct tcctgtggat gcgcagatgc 121 cctgcgtaag cgggtgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac 181 tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg 241 ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt 301 gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg catgtaaaga ctatattcgc 361 ggcgttgtga caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga 421 attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca 481 actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat 541 aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct 601 gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct 661 caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag 721 caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat 781 gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca 841 tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac 901 ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg 961 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc 1021 cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg 1081 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg 1141 cattacagtt tacgaaccga acaggcttat gtcaactggg ttcgtgcctt catccgtttc 1201 cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgccataac ttcgtatagc 1261 atacattata cgaagttatc tgtaactata acggtcctaa ggtagcgagt ttaaacacta 1321 gtatcgattc gcgacctact ccggaatatt aatagatcat ggagataatt aaaatgataa 1381 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc 1441 tataaatatt ccggattatt cataccgtcc caccatcggg cgcggatcta tggcggagtc 1501 ttcggataag ctctatcgag tcgagtacgc caagagcggg cgcgcctctt gcaagaaatg 1561 cagcgagagc atccccaagg actcgctccg gatggccatc atggtgcagt cgcccatgtt 1621 tgatggaaaa gtcccacact ggtaccactt ctcctgcttc tggaaggtgg gccactccat 1681 ccggcaccct gacgttgagg tggatgggtt ctctgagctt cggtgggatg atcagcagaa 1741 agtcaagaag acagcggaag ctggaggagt gacaggcaaa ggccaggatg gaattggtag 1801 caaggcagag aagactctgg gtgactttgc agcagagtat gccaagtcca acagaagtac 1861 gtgcaagggg tgtatggaga agatagaaaa gggccaggtg cgcctgtcca agaagatggt 1921 ggacccggag aagccacagc taggcatgat tgaccgctgg taccatccag gctgctttgt 1981 caagaacagg gaggagctgg gtttccggcc cgagtacagt gcgagtcagc tcaagggctt 2041 cagcctcctt gctacagagg ataaagaagc cctgaagaag cagctcccag gagtcaagag 2101 tgaaggaaag agaaaaggcg atgaggtgga tggagtggat gaagtggcga agaagaaatc 2161 taaaaaagaa aaagacaagg atagtaagct tgaaaaagcc ctaaaggctc agaacgacct 2221 gatctggaac atcaaggacg agctaaagaa agtgtgttca actaatgacc tgaaggagct 2281 actcatcttc aacaagcagc aagtgccttc tggggagtcg gcgatcttgg accgagtagc 2341 cgatggcatg gtgttcggtg ccctccttcc ctgcgaggaa tgctcgggtc agctggtctt 2401 caagagcgat gcctattact gcactgggga cgtcactgcc tggaccaagt gtatggtcaa 2461 gacacagaca cccaaccgga aggagtgggt aaccccaaag gaattccgag aaatctctta 2521 cctcaagaaa ttgaaggtta aaaaacagga ccgtatattc cccccagaaa ccagcgcctc 2581 cgtggcggcc acgcctccgc cctccacagc ctcggctcct gctgctgtga actcctctgc 2641 ttcagcagat aagccattat ccaacatgaa gatcctgact ctcgggaagc tgtcccggaa 2701 caaggatgaa gtgaaggcca tgattgagaa actcgggggg aagttgacgg ggacggccaa 2761 caaggcttcc ctgtgcatca gcaccaaaaa ggaggtggaa aagatgaata agaagatgga 2821 ggaagtaaag gaagccaaca tccgagttgt gtctgaggac ttcctccagg acgtctccgc 2881 ctccaccaag agccttcagg agttgttctt agcgcacatc ttgtcccctt ggggggcaga 2941 ggtgaaggca gagcctgttg aagttgtggc cccaagaggg aagtcagggg ctgcgctctc 3001 caaaaaaagc aagggccagg tcaaggagga aggtatcaac aaatctgaaa agagaatgaa 3061 attaactctt aaaggaggag cagctgtgga tcctgattct ggactggaac actctgcgca 3121 tgtcctggag aaaggtggga aggtcttcag tgccaccctt ggcctggtgg acatcgttaa 3181 aggaaccaac tcctactaca agctgcagct tctggaggac gacaaggaaa acaggtattg 3241 gatattcagg tcctggggcc gtgtgggtac ggtgatcggt agcaacaaac tggaacagat 3301 gccgtccaag gaggatgcca ttgagcactt catgaaatta tatgaagaaa aaaccgggaa 3361 cgcttggcac tccaaaaatt tcacgaagta tcccaaaaag ttctaccccc tggagattga 3421 ctatggccag gatgaagagg cagtgaagaa gctgacagta aatcctggca ccaagtccaa 3481 gctccccaag ccagttcagg acctcatcaa gatgatcttt gatgtggaaa gtatgaagaa 3541 agccatggtg gagtatgaga tcgaccttca gaagatgccc ttggggaagc tgagcaaaag 3601 gcagatccag gccgcatact ccatcctcag tgaggtccag caggcggtgt ctcagggcag 3661 cagcgactct cagatcctgg atctctcaaa tcgcttttac accctgatcc cccacgactt 3721 tgggatgaag aagcctccgc tcctgaacaa tgcagacagt gtgcaggcca aggcggaaat 3781 gcttgacaac ctgctggaca tcgaggtggc ctacagtctg ctcaggggag ggtctgatga 3841 tagcagcaag gatcccatcg atgtcaacta tgagaagctc aaaactgaca ttaaggtggt 3901 tgacagagat tctgaagaag ccgagatcat caggaagtat gttaagaaca ctcatgcaac 3961 cacacacaat gcgtatgact tggaagtcat cgatatcttt aagatagagc gtgaaggcga 4021 atgccagcgt tacaagccct ttaagcagct tcataaccga agattgctgt ggcacgggtc 4081 caggaccacc aactttgctg ggatcctgtc ccagggtctt cggatagccc cgcctgaagc 4141 gcccgtgaca ggctacatgt ttggtaaagg gatctatttc gctgacatgg tctccaagag 4201 tgccaactac tgccatacgt ctcagggaga cccaataggc ttaatcctgt tgggagaagt 4261 tgcccttgga aacatgtatg aactgaagca cgcttcacat atcagcaagt tacccaaggg 4321 caagcacagt gtcaaaggtt tgggcaaaac tacccctgat ccttcagcta acattagtct 4381 ggatggtgta gacgttcctc ttgggaccgg gatttcatct ggtgtgaatg acacctctct 4441 actatataac gagtacattg tctatgatat tgctcaggta aatctgaagt atctgctgaa 4501 actgaaattc aattttaaga cctccctgtg gggcggatct ggcggtgact ataaagatga 4561 tgatgataaa ggtggtagtg gagtcgacga gctcactgga ggtagcggac accatcacca 4621 tcaccactga tgtcgcggcc gctttcgaat ctagagcctg cagtctcgac aagcttgtcg 4681 agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa 4741 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta 4801 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 4861 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 4921 atcatgtctg gatctgatca ctgcttgagc ctagaagatc cggctgctaa caaagcccga 4981 aaggaagctg agttggctgc tgccaccgct gagcaataac tatcataacc cctagggtat 5041 acccatctaa ttggaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa 5101 ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa 5161 ataatcctta aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca 5221 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt 5281 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 5341 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 5401 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 5461 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 5521 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 5581 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 5641 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 5701 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 5761 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 5821 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 5881 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 5941 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaa //