LOCUS Exported 11746 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses FANCE.. ACCESSION . VERSION . KEYWORDS pCR1306 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11746) AUTHORS Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, Floor SN, Corn JE TITLE CRISPR-Cas9 genome editing in human cells occurs via the Fanconi anemia pathway. JOURNAL Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0. Epub 2018 Jul 27. PUBMED 30054595 REFERENCE 2 (bases 1 to 11746) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11746 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 26..130 /gene="bla" /label=AmpR promoter CDS 131..991 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(349..368) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1162..1750 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1651..1670 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1904..1921 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2038..2059 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2074..2104 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2112..2128 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2117..2139 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2136..2152 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2136..2152 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2171..2191 /label=T3 /note="T3 promoter, forward primer" promoter 2173..2191 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 2219..2445 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 2446..2626 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2673..2798 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3291..3524 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3709..3753 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4020..4136 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 4170..4194 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 4325..5503 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 4556..5494 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 5451..5471 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" promoter 5520..5538 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 5586..5612 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" primer_bind complement(7421..7438) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7648..7667 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" regulatory 7823..7832 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 7835..8323 /codon_start=1 /product="mouse GPI-anchored cell surface glycoprotein of the immunoglobulin superfamily" /label=Thy1.1 /note="This allele of Thy1 is also known as CD90.1." /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL" protein_bind complement(8329..8353) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 8384..8972 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8437..8457) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8855..8866) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 9023..9522 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(9058..9081) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 9455..9474 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 9536..10339 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(9599..9618) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 10209..10228 /label=Neo-F /note="Neomycin resistance gene, forward primer" LTR 10483..10716 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 10788..10909 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10825..10844) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10879..10898 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 10949..11084 /label=SV40 ori /note="SV40 origin of replication" primer_bind 11011..11030 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter complement(11105..11123) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(11133..11150) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(11133..11149) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(11142..11164) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 11291..11746 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(11378..11397) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 11588..11609 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg 2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc 2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt 2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg 2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta 2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca 2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg 2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta 2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta 2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac 2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa 2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag 3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag 3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga 3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat 3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact 4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata 4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg 4141 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca ggctccacca 4201 tgggaaccga cattgattat tgactaggct tttgcaaaaa gctttgcaaa gatggataaa 4261 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg 4321 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt 4381 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga 4441 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag 4501 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag 4561 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg 4621 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg 4681 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc 4741 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc 4801 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt 4861 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg 4921 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct 4981 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt 5041 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc 5101 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg 5161 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt 5221 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct 5281 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc 5341 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat 5401 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca 5461 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggaatta gcttggtact 5521 aatacgactc actataggga gacccaagct ggctaggtaa gcttggtacc gagctcggat 5581 ccatgtaccc atacgatgtt ccagattacg ctggtggatc tggaggttct ggtggaatgg 5641 cgacaccgga cgcggggctc cctggggctg agggcgtgga gccggcgccc tgggcgcagc 5701 tggaggcccc cgcccgcctc ctgctgcagg cgctgcaggc ggggcctgag ggggcgcggc 5761 gcggcctggg ggtgctccgg gcgctgggca gccgcggctg ggagcccttc gactggggtc 5821 gcttgctcga ggccctgtgc cgggaggagc cggtcgtgca ggggcctgac ggccgtctgg 5881 agctgaaacc actgttgctg cgattgcccc ggatatgcca gaggaacctg atgtccctgc 5941 tgatggccgt tcggccatcg ctgccggaaa gtgggctcct ctctgtgctg cagattgccc 6001 agcaggacct agcccctgac cccgatgcct ggctccgtgc cctgggggaa ttgctgcgaa 6061 gggatttggg ggtggggacc tccatggagg gagcttctcc actgtctgaa agatgccaga 6121 gacagctcca aagtctatgt agggggctgg gcctgggggg caggaggttg aaatcccccc 6181 aggctccaga ccctgaagaa gaggagaaca gggactccca gcagcctggg aaacgcagaa 6241 aggactcaga ggaagaggct gccagtcctg aggggaagag ggtccccaaa agattacggt 6301 gttgggaaga ggaagaagat catgagaagg agagacccga acataagtca ctggaatccc 6361 tggcagatgg aggaagtgca tctcctatta aggaccagcc tgtcatggca gttaagactg 6421 gcgaggacgg ttcgaatctg gatgatgcta aaggtctggc tgagagtttg gagttgccca 6481 aagctatcca ggaccagctt cccaggctgc agcagctgct gaagaccttg gaggaggggt 6541 tagagggatt ggaggatgcc cccccagttg agctacagct tcttcacgaa tgtagtccca 6601 gccagatgga cttgctgtgt gcccagctgc agctccctca gctctcagac ctcggtctcc 6661 tgcggctctg cacctggctg ctggcccttt cacctgatct cagcctcagc aatgctactg 6721 tgctgaccag aagcctcttt cttggacgga tcctctcctt gacttcctca gcctcccgcc 6781 tgcttacaac tgccctgacc tccttctgtg ccaaatatac ataccctgtc tgcagcgccc 6841 tccttgaccc tgtgctccag gccccaggca caggtcctgc tcaaacagag ttactgtgtt 6901 gccttgtgaa gatggagtcc ctggagccag atgcacaggt tctaatgctg ggacagatct 6961 tggagctgcc ctggaaggag gaaactttct tggtgttgca gtcactccta gagcggcagg 7021 tggagatgac ccctgagaag ttcagtgtct taatggagaa gctctgtaaa aaggggctgg 7081 cagccaccac ctccatggcc tatgccaagc tcatgctgac agtgatgacc aagtatcagg 7141 ctaacatcac tgagacccag aggctgggcc tggctatggc cctagaacct aacaccacct 7201 tcctgaggaa gtccctgaag gccgccttga aacatttggg cccctgagtc gaccccctct 7261 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt 7321 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct 7381 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa 7441 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg 7501 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc 7561 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg 7621 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg 7681 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc 7741 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg 7801 gttttccttt gaaaaacacg atgccaccat gggtatgaac ccagccatca gcgtcgctct 7861 cctgctctca gtcttgcagg tgtcccgagg gcagaaggtg accagcctga cagcctgcct 7921 ggtgaaccaa aaccttcgcc tggactgccg ccatgagaat aacaccaagg ataactccat 7981 ccagcatgag ttcagcctga cccgagagaa gaggaagcac gtgctctcag gcacccttgg 8041 gatacccgag cacacgtacc gctcccgcgt caccctctcc aaccagccct atatcaaggt 8101 ccttacccta gccaacttca ccaccaagga tgagggcgac tacttttgtg agcttcgcgt 8161 ctcgggcgcg aatcccatga gctccaataa aagtatcagt gtgtatagag acaagctggt 8221 caagtgtggc ggcataagcc tgctggttca gaacacatcc tggatgctgc tgctgctgct 8281 ttccctctcc ctcctccaag ccctggactt catttctctg tgatctagac ccagctttct 8341 tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gacaatcaac ctctggatta 8401 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 8461 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 8521 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 8581 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 8641 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 8701 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 8761 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg 8821 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 8881 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 8941 gagtcggatc tccctttggg ccgcctcccc gcctggacct gcaggattct gcagatatcc 9001 ggactagtga tctaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca 9061 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca 9121 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca 9181 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg 9241 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag 9301 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc 9361 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc 9421 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg 9481 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag ccaatatggg 9541 atcggccatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 9601 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 9661 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 9721 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 9781 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 9841 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 9901 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 9961 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 10021 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc 10081 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 10141 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 10201 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 10261 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 10321 tcttgacgag ttcttctgaa cgcgtcgccc gccccacgac ccgcagcgcc cgaccgaaag 10381 gagcgcacga ccccatgcat ctcgagggcc cggtaccttt aagaccaatg acttacaagg 10441 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact 10501 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 10561 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 10621 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 10681 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 10741 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg 10801 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 10861 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc 10921 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 10981 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 11041 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat 11101 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac 11161 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 11221 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 11281 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 11341 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 11401 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 11461 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 11521 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 11581 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 11641 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 11701 caaaaattta acgcgaattt taacaaaata ttaacgctta caattt //