LOCUS Exported 5493 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION An AAV vector that expresses green pre-eGRASP(p32) under the CaMKIIa promoter.. ACCESSION . VERSION . KEYWORDS pAAV-CWB-pre-eGRASP(p32) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5493) AUTHORS Choi JH, Sim SE, Kim JI, Choi DI, Oh J, Ye S, Lee J, Kim T, Ko HG, Lim CS, Kaang BK TITLE Interregional synaptic maps among engram cells underlie memory formation. JOURNAL Science. 2018 Apr 27;360(6387):430-435. doi: 10.1126/science.aas9204. PUBMED 29700265 REFERENCE 2 (bases 1 to 5493) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5493 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 79..537 /codon_start=1 /product="improved N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VN155(I152L) /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKF ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKRHDFFKSAMPEGYVQERTISFKDDGK YKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITA" primer_bind complement(118..139) /label=EGFP-N /note="EGFP, reverse primer" misc_feature 1368..1956 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(1421..1441) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(1839..1850) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 1969..2193 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 2201..2341 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 2201..2330 /label=AAV2 ITR rep_origin 2416..2871 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2503..2522) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2713..2734 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2888..2907) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3007..3029 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3067..3085) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3153..3257 /gene="bla" /label=AmpR promoter CDS 3258..4118 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3476..3495) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4289..4877 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4778..4797 /label=pBR322ori-F /note="pBR322 origin, forward primer" repeat_region 4939..5068 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" regulatory 5488..4 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 atggagaccg acaccctcct gctatgggtg ctgctgctct gggtgcccgg cagcaccggc 61 gacgcaccgg tcggaggcag caagggcgag gagctgttca ccggggtggt gcccatcctg 121 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgaggggcga gggcgagggc 181 gatgccacca tcggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 241 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcgc ccgctacccc 301 gaccacatga agcggcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 361 cgcaccatct ccttcaagga cgacggcaag tacaagaccc gcgccgtggt gaagttcgag 421 ggcgacaccc tggtgaaccg catcgagctg aagggcaccg acttcaagga ggacggcaac 481 atcctggggc acaagctgga gtacaacttc aacagccaca acgtctatat caccgccgac 541 aagcagaaga acggcatcaa ggccaacttc accgtgcgcc acaacgtgga ggacggcagc 601 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 661 cccgacaacc actacctgag cacccagacc gtgctgagca aagaccccaa cgagaagacc 721 ggtggatctg gaggcagtgg cggatctaga tctcctagct actctccacc acctccacct 781 ggaggaggtt ccggaggcgg aagcggtacc gaagtgcctt cctctatgac aactgagtcg 841 acagccactg ccatgcagtc cgagatgtcc acctcaatca tggagaccac cacaaccctg 901 gctaccagca cagctcgacg aggaaagccc cccacaaagg agcctatcag ccagaccacg 961 gatgatatcc ttgtggcctc ggcagagtgt cccagcgacg atgaggacat tgacccctgt 1021 gagccgagct caggtgggtt agccaacccc acccgagtgg gcggccgcga accataccca 1081 ggctcggcag aggtgatccg ggagtctagc agtaccactg gcatggtggt ggggattgtc 1141 gcagcagcag ctctgtgcat cctcatcctc ctctatgcca tgtacaagta cagaaaccgg 1201 gatgaaggct catatcacgt ggatgagagt cgaaactaca tcagtaactc agcacagtcc 1261 aatggggctg tggtcaagga gaagcagccc agcagtgcga aaagcgccaa caaaaacaag 1321 aagaacaagg ataaggagta ttatgtctga attcctgcag ccccgataat caacctctgg 1381 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 1441 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 1501 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 1561 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 1621 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 1681 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 1741 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca 1801 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 1861 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc 1921 agacgagtcg gatctccctt tgggccgcct ccccgcatcg gggggatcct gtgccttcta 1981 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca 2041 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc 2101 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata 2161 gcaggcatgc tggggatgcg gtgggctcta tggcggccgc aggaacccct agtgatggag 2221 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc 2281 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag 2341 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt 2401 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 2461 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc 2521 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 2581 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg 2641 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 2701 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct 2761 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga 2821 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca 2881 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac 2941 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga 3001 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac 3061 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt 3121 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 3181 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 3241 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 3301 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 3361 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 3421 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 3481 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 3541 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 3601 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 3661 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 3721 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 3781 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 3841 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 3901 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 3961 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 4021 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 4081 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 4141 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 4201 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 4261 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 4321 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 4381 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 4441 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 4501 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 4561 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 4621 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 4681 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 4741 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 4801 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 4861 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 4921 ggccttttgc tcacatgtcc tgcaggcagc tgcgcgctcg ctcgctcact gaggccgccc 4981 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga 5041 gtggccaact ccatcactag gggttcctgc ggccgcagat gctgcacact tgtggactaa 5101 gtttgttcgc atccccttct ccaaccccct cagtacatca ccctggggga acagggtcca 5161 cttgctcctg ggcccacaca gtcctgcagt attgtgtata taaggccagg gcaaagagga 5221 gcaggtttta aagtgaaagg caggcaggtg ttggggaggc agttaccggg gcaacgggaa 5281 cagggcgttt cggaggtggt tgccatgggg acctggatgc tgacgaaggc tcgcgaggct 5341 gtgagcagcc acagtgccct gctcagaagc cccaagctcg tcagtcaagc cggttctccg 5401 tttgcactca ggagcacggg caggcgagtg gcccctagtt ctgggggcag cgtgaaaaga 5461 cggtatcgat aagcttgatt cgagctagcc acc //