LOCUS Exported 5696 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Baculovirus expression for structure determination; may not be full ORF. ACCESSION . VERSION . KEYWORDS LIMK2 (LIMK2A-c052) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5696) TITLE SGC Oxford April 2018 plasmids REFERENCE 2 (bases 1 to 5696) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5696 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..92 /gene="polh from Autographa californica" /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" primer_bind 17..34 /label=Polyhedrin-F /note="Polyhedrin promoter, forward primer" CDS 154..171 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 196..216 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQS" polyA_signal 1302..1436 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1339..1358) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1393..1412 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" mobile_element 1465..1630 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" rep_origin 1814..2268 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1901..1920) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2110..2131 /label=F1ori-F /note="F1 origin, forward primer" promoter 2294..2398 /gene="bla" /label=AmpR promoter CDS 2399..3259 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2617..2636) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3430..4018 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3919..3938 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4172..4189 /label=L4440 /note="L4440 vector, forward primer" mobile_element 4323..4547 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (right end of the Tn7 transposon)" promoter complement(5317..5345) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" ORIGIN 1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc 61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca 121 tcgggcgcgg atctcggtcc gaaaaccatg ggccaccatc atcatcatca ttcttctggt 181 gtagatctgg gtaccgagaa cctgtacttc caatccatgg acctaatcca tggggaggtc 241 ctggggaagg gcttctttgg gcaggctatc aaggtgacac acaaagccac gggcaaagtg 301 atggtcatga aagagttaat tcgatgtgat gaggagaccc agaaaacttt tctgactgag 361 gtgaaagtga tgcgcagcct ggaccacccc aatgtgctca agttcattgg tgtgctgtac 421 aaggataaga agctgaacct gctgacagag tacattgagg ggggcacact gaaggacttt 481 ctgcgcagta tggatccgtt cccctggcag cagaaggtca ggtttgccaa aggaatcgcc 541 tccggaatgg cctatttgca ctctatgtgc atcatccacc gggatctgaa ctcgcacaac 601 tgcctcatca agttggacaa gactgtggtg gtggcagact ttgggctgtc acggctcata 661 gtggaagaga ggaaaagggc ccccatggag aaggccacca ccaagaaacg caccttgcgc 721 aagaacgacc gcaagaagcg ctacacggtg gtgggaaacc cctactggat ggcccctgag 781 atgctgaacg gaaagagcta tgatgagacg gtggatatct tctcctttgg gatcgttctc 841 tgtgagatca ttgggcaggt gtatgcagat cctgactgcc ttccccgaac actggacttt 901 ggcctcaacg tgaagctttt ctgggagaag tttgttccca cagattgtcc cccggccttc 961 ttcccgctgg ccgccatctg ctgcagactg gagcctgaga gcagaccagc attctcgaaa 1021 ttggaggact cctttgaggc cctctccctg tacctggggg agctgggcat cccgctgcct 1081 gcagagctgg aggagttgga ccacactgtg agcatgcagt acggcctgtg acagtaaagg 1141 tggatacgga tccgaattcg agctccgtcg acaagcttgt cgagaagtac tagaggatca 1201 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc 1261 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt 1321 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 1381 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat 1441 cactgatatc gcctaggaga tccgaaccag ataagtgaaa tctagttcca aactattttg 1501 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac 1561 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg 1621 tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat cctgccaact 1681 ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga aaatttttct 1741 gtcatctctt cgttattaat gtttgtaatt gactgaatat caacgcttat ttgcagcctg 1801 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 1861 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 1921 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 1981 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 2041 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 2101 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 2161 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 2221 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt 2281 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 2341 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 2401 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 2461 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 2521 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 2581 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 2641 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 2701 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 2761 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 2821 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 2881 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 2941 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 3001 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 3061 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 3121 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 3181 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 3241 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 3301 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 3361 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 3421 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 3481 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 3541 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 3601 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 3661 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 3721 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 3781 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 3841 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 3901 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 3961 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 4021 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 4081 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 4141 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 4201 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca 4261 gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg 4321 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa 4381 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc 4441 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg 4501 tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac 4561 aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg 4621 gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag 4681 agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc 4741 gcgttggcct catgcttgag cagattgatg agcgcggtgg caatgccctg cctccggtgc 4801 tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc 4861 agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg 4921 aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag 4981 gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact 5041 tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt 5101 ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc 5161 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca 5221 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg 5281 gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat 5341 gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc 5401 agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc 5461 acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat 5521 ctgccctggc ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg 5581 accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc 5641 caggactcta gctatagttc tagtggttgg ctacgtatac tccggaatat taatag //