LOCUS Exported 5921 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Baculovirus expression for structure determination; may not be full ORF. ACCESSION . VERSION . KEYWORDS WNK3 (WNK3B-c001) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5921) TITLE SGC Oxford April 2018 plasmids REFERENCE 2 (bases 1 to 5921) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5921 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..92 /gene="polh from Autographa californica" /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" primer_bind 17..34 /label=Polyhedrin-F /note="Polyhedrin promoter, forward primer" CDS 154..171 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 196..216 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQS" polyA_signal 1527..1661 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1564..1583) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1618..1637 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" mobile_element 1690..1855 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" rep_origin 2039..2493 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2126..2145) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2335..2356 /label=F1ori-F /note="F1 origin, forward primer" promoter 2519..2623 /gene="bla" /label=AmpR promoter CDS 2624..3484 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2842..2861) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3655..4243 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4144..4163 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4397..4414 /label=L4440 /note="L4440 vector, forward primer" mobile_element 4548..4772 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (right end of the Tn7 transposon)" promoter complement(5542..5570) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" ORIGIN 1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc 61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca 121 tcgggcgcgg atctcggtcc gaaaaccatg ggccaccatc atcatcatca ttcttctggt 181 gtagatctgg gtaccgagaa cctgtacttc caatccatgg agaaaaatga aaaggaaatg 241 gaagaagaag cagaaatgaa ggctgtagct acttctccta gtggcagatt cctgaaattt 301 gacatagaac taggaagagg agcatttaaa acagtatata aaggactgga cactgaaaca 361 tgggttgagg ttgcttggtg tgagctgcag gaccgaaagt taaccaaagc tgagcagcaa 421 agattcaagg aagaagcaga gatgttgaag ggtctccagc accccaatat agttcgattt 481 tatgattcct gggaatctat attaaaagga aagaaatgta ttgtattagt gactgaacta 541 atgacatctg ggaccttaaa gacgtactta aaacgattta aagtcatgaa accaaaggtc 601 ttaaggagct ggtgcaggca aattttaaag gggttgcagt tcttgcacac taggactcct 661 cctattattc accgggatct gaagtgtgac aatattttca tcacgggacc cactggatct 721 gtgaagattg gtgatctagg attagccacc ttaatgcgta cctcatttgc taagagtgtc 781 attggaactc ctgagtttat ggctccagag atgtatgaag aacactatga tgaatccgta 841 gatgtttatg cttttggaat gtgtatgctg gaaatggcca catcggagta tccttattct 901 gagtgtcaga atgcagctca aatataccgg aaagtaacta gtggcataaa accagccagc 961 ttcaataaag tcactgatcc tgaagtcaaa gaaatcattg aaggatgtat tcgtcaaaac 1021 aaatctgaaa ggttgtctat cagggaccta ttaaaccatg cattttttgc tgaggataca 1081 ggactgaggg tggagttagc agaagaagat gattgctcaa attcatccct tgctttaaga 1141 ctctgggttg aagaccctaa aaaattgaaa ggcaaacaca aagacaatga agctattgaa 1201 tttagtttca acttagaaac agatacacct gaggaagtag catatgaaat ggtcaagtct 1261 gggttcttcc atgaaagtga ttccaaagct gttgctaaat ccattagaga ccgggtgacg 1321 ccaataaaga agacaagaga gaagaagcct gcttgacagt aaaggtggat acggatccga 1381 attcgagctc cgtcgacaag cttgtcgaga agtactagag gatcataatc agccatacca 1441 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac 1501 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat 1561 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 1621 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg atatcgccta 1681 ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc 1741 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat 1801 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagcggg 1861 gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg tgacaaaccg 1921 tcatcttcgg ctactttttc tctgtcacag aatgaaaatt tttctgtcat ctcttcgtta 1981 ttaatgtttg taattgactg aatatcaacg cttatttgca gcctgaatgg cgaatgggac 2041 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 2101 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 2161 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 2221 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 2281 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt aatagtggac 2341 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 2401 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 2461 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg 2521 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 2581 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 2641 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 2701 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 2761 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 2821 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 2881 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 2941 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 3001 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 3061 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 3121 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 3181 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 3241 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 3301 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 3361 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 3421 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 3481 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 3541 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 3601 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 3661 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 3721 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 3781 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 3841 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 3901 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 3961 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 4021 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 4081 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 4141 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 4201 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 4261 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 4321 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 4381 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta 4441 ttttctcctt acgcatctgt gcggtatttc acaccgcata gaccagccgc gtaacctggc 4501 aaaatcggtt acggttgagt aataaatgga tgccctgcgt aagcgggtgt gggcggacaa 4561 taaagtctta aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca 4621 gaatagttgt aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt 4681 tatggctaaa gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg 4741 gcgtggccaa gggcatggta aagactatat tcgcggcgtt gtgacaattt accgaacaac 4801 tccgcggccg ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg 4861 atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga 4921 tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc 4981 ttgagcagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg 5041 agatcataga tatagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg 5101 agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg 5161 agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg 5221 aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc 5281 ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc 5341 tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct 5401 tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa 5461 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag 5521 cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt 5581 gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag 5641 gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca 5701 ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc ctggcttcag 5761 gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg 5821 gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagga ctctagctat 5881 agttctagtg gttggctacg tatactccgg aatattaata g //