LOCUS       Exported               10350 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses Cas9 in T. brucei (2T1) cells.
ACCESSION   .
VERSION     .
KEYWORDS    pRPaCas9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10350)
  AUTHORS   Rico E, Jeacock L, Kovarova J, Horn D
  TITLE     Inducible high-efficiency CRISPR-Cas9-targeted gene editing and 
            precision base editing in African trypanosomes.
  JOURNAL   Sci Rep. 2018 May 21;8(1):7960. doi: 10.1038/s41598-018-26303-w.
  PUBMED    29785042
REFERENCE   2  (bases 1 to 10350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10350
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(760..1620)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1793..1811
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1849..1871)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1971..1990
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2200..2219
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             4257..8360
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     terminator      9299..9346
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      9431..9478
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(10180..10197)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtggt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     1801 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     1861 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     1921 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     1981 gcagattgta ctgagagtgc accattcgac gctctccctt atgcgactcc tgcattagga
     2041 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcaagga
     2101 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
     2161 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
     2221 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
     2281 ctggctagga atactgcata gataacaaac gcatcaaaca caactggtgg cactatttca
     2341 atcatgtcga cggcgcgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
     2401 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
     2461 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
     2521 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata
     2581 cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg
     2641 ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga
     2701 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag
     2761 gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc
     2821 ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac
     2881 agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt
     2941 gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc
     3001 cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc
     3061 aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc
     3121 gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg
     3181 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc
     3241 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag
     3301 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc
     3361 aggctttttc atcactagac ttcgttgcag ttgagtttct gttttcgggc ttgttggtat
     3421 acttgtttga ttaatgtggc tgatagctaa gctatcgcaa atatttgtac aagacatgag
     3481 ggggcaaaaa aataagccaa gtgtatttgt taggcttccg tgcctgttag agaaatatgt
     3541 aaggaaacgc gtgcgctaca cagcttgagc acccatcgaa ttgggacgag aacatgttaa
     3601 actcagtact cacccgataa cccctatttt caatgcacgc ccacccagag acacggaata
     3661 gcataaaaca accccatcac tgccacaaaa tggacatttt gcacaaaacg cactattgac
     3721 tagtattcga ctatactaat aagagatacc tatcagacac gatatactgt gaaaaggaca
     3781 ttaagctcac tatgcatctg aaaacgcttt cacctcacaa cctacttaca cacacctttc
     3841 cgcttaaccc gctagctttc cacccagcgc gggtgcattc tggctcttat atatacttat
     3901 tgtcatgaca gagtatattg tactgtgttg ataagggacg ggtaactgta ttgaagagcc
     3961 gatgcttttg acatgttaga tataatatgt tttattgtaa agtcaataca acacacaata
     4021 ggataataat gataaagtta aaaaagtata tatagtaata gaaatatatc ttatatagga
     4081 aagattaagc agtaaaagta gcgcttacgg cgtacaccct atcaatgata gagtggtacc
     4141 ctgcacgcgc cttcgagttt tttttccttt tccccatttt tttcaacttg aagacttcaa
     4201 ttacaccaaa aagtaaaatt cacaagctta tgcgaggaca caagcggtca cgtgaagata
     4261 agaagtactc gatcggcctg gacatcggca cgaactcggt gggctgggcg gtgatcacgg
     4321 acgagtacaa ggtgccgtcg aagaagttca aggtgctggg caacacggac cgccactcga
     4381 tcaagaagaa cctgatcggc gcgctgctgt tcgactcggg cgagacggcg gaggcgacgc
     4441 gcctgaagcg cacggcccgc cgccgctaca cgcgccgcaa gaaccgcatc tgctacctgc
     4501 aggagatttt ctcgaacgag atggcgaagg tggacgactc gttcttccac cgcctggagg
     4561 agtcgttcct ggtggaggag gacaagaagc acgagcgcca cccgatcttc ggcaacatcg
     4621 tggacgaggt ggcgtaccac gagaagtacc cgacgatcta ccacctgcgc aagaagctgg
     4681 tggactcgac ggacaaggcg gacctgcgcc tgatctacct ggcgctggcg cacatgatca
     4741 agttccgcgg ccacttcctg atcgagggcg acctgaaccc ggacaactcg gacgtggaca
     4801 agctgttcat ccagctggtg cagacgtaca accagctgtt cgaggagaac ccgatcaacg
     4861 cgtcgggcgt ggacgcgaag gcgatcctgt cggcccgcct gtcgaagtcg cgccgcctgg
     4921 agaacctgat cgcgcagctg ccgggcgaga agaagaacgg cctgttcggc aacctgatcg
     4981 cgctgtcgct gggcctgacg ccgaacttca agtcgaactt cgacctggcg gaggacgcga
     5041 agctgcagct gtcgaaggac acgtacgacg acgacctgga caacctgctg gcgcagatcg
     5101 gcgaccagta cgcggacctg ttcctggcgg cgaagaacct gtcggacgcg atcctgctgt
     5161 cggacatcct gcgcgtgaac acggagatca cgaaggcgcc gctgtcggcg tcgatgatca
     5221 agcgctacga cgagcaccac caggacctga cgctgctgaa ggcgctggtg cgccagcagc
     5281 tgccggagaa gtacaaggag attttcttcg accagtcgaa gaacggctac gcgggctaca
     5341 tcgacggcgg cgcgtcgcag gaggagttct acaagttcat caagccgatc ctggagaaga
     5401 tggacggcac ggaggagctg ctggtgaagc tgaaccgcga ggacctgctg cgcaagcagc
     5461 gcacgttcga caacggctcg atcccgcacc agatccacct gggcgagctg cacgcgatcc
     5521 tgcgccgcca ggaggacttc tacccgttcc tgaaggacaa ccgcgagaag atcgagaaga
     5581 tcctgacgtt ccgcatcccg tactacgtgg gcccgctggc gcgcggcaac tcgcgcttcg
     5641 cgtggatgac gcgcaagtcg gaggagacga tcacgccgtg gaacttcgag gaggtggtgg
     5701 acaagggcgc gtcggcgcag tcgttcatcg agcgcatgac gaacttcgac aagaacctgc
     5761 cgaacgagaa ggtgctgccg aagcactcgc tgctgtacga gtacttcacg gtgtacaacg
     5821 agctgacgaa ggtgaagtac gtgacggagg gcatgcgcaa gccggcgttc ctgtcgggcg
     5881 agcagaagaa ggcgatcgtg gacctgctgt tcaagacgaa ccgcaaggtg acggtgaagc
     5941 agctgaagga ggactacttc aagaagatcg agtgcttcga ctcggtggag atttcgggcg
     6001 tggaggaccg cttcaacgcg tcgctgggca cgtaccacga cctgctgaag atcatcaagg
     6061 acaaggactt cctggacaac gaggagaacg aggacatcct ggaggacatc gtgctgacgc
     6121 tgacgctgtt cgaggaccgc gagatgatcg aggagcgcct gaaaacgtac gcgcacctgt
     6181 tcgacgacaa ggtgatgaag cagctgaagc gccgccgcta cacgggctgg ggccgcctgt
     6241 cgcgcaagct gatcaacggc atccgcgaca agcagtcggg caagacgatc ctggacttcc
     6301 tgaagtcgga cggcttcgcg aaccgcaact tcatgcagct gatccacgac gactcgctga
     6361 cgttcaagga ggacatccag aaggcgcagg tgtcgggcca gggcgactcg ctgcacgagc
     6421 acatcgcgaa cctggcgggc tcgccggcga tcaagaaggg catcctgcag acggtgaagg
     6481 tggtggacga gctggtgaag gtgatgggcc gccacaagcc ggagaacatc gtgatcgaga
     6541 tggcgcgcga gaaccagacg acgcagaagg gccagaagaa ctcgcgcgag cgcatgaagc
     6601 gcatcgagga gggcatcaag gagctgggct cgcagatcct gaaggagcac ccggtggaga
     6661 acacgcagct gcagaacgag aagctgtacc tgtactacct gcagaacggc cgcgacatgt
     6721 acgtggacca ggagctggac atcaaccgcc tgtcggacta cgacgtggac cacatcgtgc
     6781 cgcagtcgtt cctgaaggac gactcgatcg acaacaaggt gctgacgcgc tcggacaaga
     6841 accgcggcaa gtcggacaac gtgccgtcgg aggaggtggt gaagaagatg aagaactact
     6901 ggcgccagct gctgaacgcg aagctgatca cgcagcgcaa gttcgacaac ctgacgaagg
     6961 cggagcgcgg cggcctgtcg gagctggaca aggcgggctt catcaagcgc cagctggtgg
     7021 agacgcgcca gatcacgaag cacgtggcgc agatcctgga ctcgcgcatg aacacgaagt
     7081 acgacgagaa cgacaagctg atccgcgagg tgaaggtgat cacgctgaag tcgaagctgg
     7141 tgtcggactt ccgcaaggac ttccagttct acaaggtgcg cgagatcaac aactaccacc
     7201 acgcgcacga cgcgtacctg aacgcggtgg tgggcacggc gctgatcaag aagtacccga
     7261 agctggagtc ggagttcgtg tacggcgact acaaggtgta cgacgtgcgc aagatgatcg
     7321 cgaagtcgga gcaggagatc ggcaaggcga cggcgaagta cttcttctac tcgaacatca
     7381 tgaacttctt caagacggag atcacgctgg cgaacggcga gatccgcaag cgcccgctga
     7441 tcgagacgaa cggcgagacg ggcgagatcg tgtgggacaa gggccgcgac ttcgcgacgg
     7501 tgcgcaaggt gctgtcgatg ccgcaggtga acatcgtgaa gaaaacggag gtgcagacgg
     7561 gcggcttctc gaaggagtcg atcctgccga agcgcaactc ggacaagctg atcgcgcgca
     7621 agaaggactg ggacccgaag aagtacggcg gcttcgactc gccgacggtg gcgtactcgg
     7681 tgctggtggt ggcgaaggtg gagaagggca agtcgaagaa gctgaagtcg gtgaaggagc
     7741 tgctgggcat cacgatcatg gagcgctcgt cgttcgagaa gaacccgatc gacttcctgg
     7801 aggcgaaggg ctacaaggag gtgaagaagg acctgatcat caagctgccg aagtactcgc
     7861 tgttcgagct ggagaacggc cgcaagcgca tgctggcgtc ggcgggcgag ctgcagaagg
     7921 gcaacgagct ggcgctgccg tcgaagtacg tgaacttcct gtacctggcg tcgcactacg
     7981 agaagctgaa gggctcgccg gaggacaacg agcagaagca gctgttcgtg gagcagcaca
     8041 agcactacct ggacgagatc atcgagcaga tttcggagtt ctcgaagcgc gtgatcctgg
     8101 cggacgcgaa cctggacaag gtgctgtcgg cgtacaacaa gcaccgcgac aagccgatcc
     8161 gcgagcaggc ggagaacatc atccacctgt tcacgctgac gaacctgggc gccccggcgg
     8221 cgttcaagta cttcgacacg acgatcgacc gcaagcgcta cacgtcgacg aaggaggtgc
     8281 tggacgcgac gctgatccac cagtcgatca cgggcctgta cgagacgcgc atcgacctgt
     8341 cgcagctggg cggcgactga ctcgagagat cctgcccatt tagttggctt ttcccttgtc
     8401 tcgtgtcttt tccgtggaaa ggttcccgga gtaatctgat ggcacagcag ggaagtgcgc
     8461 ctgcaggttg gttaggaagg ggggatgatg taaaagaaga aaatgggggg atatcttatg
     8521 tttaagagga taaataatgt gaaggggctt tatatgcttg cttggttgtc tcgttggtgc
     8581 atggggaatc tgcatgtttg ctttggagca cgcgtggtac atcaatggga tcatatcttg
     8641 ctgcctcccg cagtcaccgt gcggagctgc cggtgccccg tttctcatag gcgtgtgcac
     8701 cttccgtctg agacctgtaa atggttaaaa aggaatatat aattaccttt tgaaaagtgg
     8761 taaaaaacga atatatgttt ttttttggtt ttaatacgct cttttgtgtg tatgagagga
     8821 ataaataagt gtgtgtgtgt gtgtgttgtt gttgttgttg aaaaaatgaa ggacacgaaa
     8881 gcgctcatta agggtgggga tacaccaggc ataattatcc gaaaatattt tgatgcacca
     8941 aataagtgaa catactgacg aaatcaaaga gttggaggat ggatagggag gcctcattgg
     9001 cagtggtaaa ttgatttgac ttgaatatga taccctctta ctgtttcatt gccactacaa
     9061 gttggtttcc ttcccctgca ggcatgcaag ctagtcgagc cacacctatc agccggtgtg
     9121 ttgccaaaga cattctgtat aattttccaa ctaccctaac ctgattcact catcgttgag
     9181 catttgttct tctacttttt gaccgcaagt gtcgttatta gtattcggtc ctgggctgca
     9241 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     9301 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggagaact
     9361 atatccggat agggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     9421 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     9481 gaaaggagaa ctatatccgg attgggcccc tgcaggaatt cgatgcgagg cgaatcgctc
     9541 agtagtgtta gtgcaggcat tgtttctctt ttccatttcg gctcgctctc tgtgccagaa
     9601 aggcttttgg agtcttggtt actcgtggga ctagggcaag cgtcgcttcc ctaataattg
     9661 ttgctaacgt ttcaccgtgc gtgtgtggat atcttccaag ttgtcatttt ctctacccct
     9721 ctcttttccg gcgcggtcta acttcctcca actagcgcat ttccatgata acggggtcca
     9781 catagtccgt tgtagtgaaa ttggcatgcc atcagggcca aggcatacat cgcggccgcg
     9841 ataaaaatga tacgatgatc tccgtgtgga gcagaccgtg gcgaccagtt tgtaccatgg
     9901 aagttcttcg atagagttat atcgagtgcg gaattgatgc tacggtcaca gtgtcgtatc
     9961 gtgtggtctc ccatgtgttt ttgacggtgt accaattatc tttgcactaa tgggctatgg
    10021 aatctttcct tttaatcagg gggacatgtt tatcccacgc cagtagtctg gaattagaat
    10081 acataccaac tatatggcgc gccgagctcg aattctgcat taatgaatcg gccaacgcgc
    10141 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    10201 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    10261 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    10321 gaaccgtaaa aaggccgcgt tgctggcgtt
//
