LOCUS Exported 5493 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION An AAV vector that expresses yellow pre-eGRASP(p30) under the CaMKIIa promoter.. ACCESSION . VERSION . KEYWORDS pAAV-CWB-yellow pre-eGRASP(p30) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5493) AUTHORS Choi JH, Sim SE, Kim JI, Choi DI, Oh J, Ye S, Lee J, Kim T, Ko HG, Lim CS, Kaang BK TITLE Interregional synaptic maps among engram cells underlie memory formation. JOURNAL Science. 2018 Apr 27;360(6387):430-435. doi: 10.1126/science.aas9204. PUBMED 29700265 REFERENCE 2 (bases 1 to 5493) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5493 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 420..429 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 504..962 /codon_start=1 /product="improved N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VN155(I152L) /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKF ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKRHDFFKSAMPEGYVQERTISFKDDGK YKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITA" primer_bind complement(543..564) /label=EGFP-N /note="EGFP, reverse primer" misc_feature 1793..2381 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(1846..1866) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2264..2275) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 2394..2618 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 2626..2766 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 2626..2755 /label=AAV2 ITR rep_origin 2841..3296 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2928..2947) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3138..3159 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3313..3332) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3432..3454 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3492..3510) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3578..3682 /gene="bla" /label=AmpR promoter CDS 3683..4543 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3901..3920) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4714..5302 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5203..5222 /label=pBR322ori-F /note="pBR322 origin, forward primer" repeat_region 5364..5493 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" ORIGIN 1 gcggccgcag atgctgcaca cttgtggact aagtttgttc gcatcccctt ctccaacccc 61 ctcagtacat caccctgggg gaacagggtc cacttgctcc tgggcccaca cagtcctgca 121 gtattgtgta tataaggcca gggcaaagag gagcaggttt taaagtgaaa ggcaggcagg 181 tgttggggag gcagttaccg gggcaacggg aacagggcgt ttcggaggtg gttgccatgg 241 ggacctggat gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa 301 gccccaagct cgtcagtcaa gccggttctc cgtttgcact caggagcacg ggcaggcgag 361 tggcccctag ttctgggggc agcgtgaaaa gacggtatcg ataagcttga ttcgagctag 421 ccaccatgga gaccgacacc ctcctgctat gggtgctgct gctctgggtg cccggcagca 481 ccggcgacgc accggtcgga ggcagcaagg gcgaggagct gttcaccggg gtggtgccca 541 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgagg ggcgagggcg 601 agggcgatgc caccatcggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 661 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcgcccgct 721 accccgacca catgaagcgg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 781 aggagcgcac catctccttc aaggacgacg gcaagtacaa gacccgcgcc gtggtgaagt 841 tcgagggcga caccctggtg aaccgcatcg agctgaaggg caccgacttc aaggaggacg 901 gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaacgtc tatatcaccg 961 ccgacaagca gaagaacggc atcaaggcca acttcaccgt gcgccacaac gtggaggacg 1021 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 1081 tgctgcccga caaccactac ctgagctacc agaccgtgct gagcaaagac cccaacgaga 1141 agaccggtgg atctggaggc agtggcggat ctagagctcc tacaaagcct ccaccactgc 1201 cacctggagg aggttccgga ggcggaagcg gtaccgaagt gccttcctct atgacaactg 1261 agtcgacagc cactgccatg cagtccgaga tgtccacctc aatcatggag accaccacaa 1321 ccctggctac cagcacagct cgacgaggaa agccccccac aaaggagcct atcagccaga 1381 ccacggatga tatccttgtg gcctcggcag agtgtcccag cgacgatgag gacattgacc 1441 cctgtgagcc gagctcaggt gggttagcca accccacccg agtgggcggc cgcgaaccat 1501 acccaggctc ggcagaggtg atccgggagt ctagcagtac cactggcatg gtggtgggga 1561 ttgtcgcagc agcagctctg tgcatcctca tcctcctcta tgccatgtac aagtacagaa 1621 accgggatga aggctcatat cacgtggatg agagtcgaaa ctacatcagt aactcagcac 1681 agtccaatgg ggctgtggtc aaggagaagc agcccagcag tgcgaaaagc gccaacaaaa 1741 acaagaagaa caaggataag gagtattatg tctgaattcc tgcagccccg ataatcaacc 1801 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 1861 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 1921 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 1981 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 2041 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 2101 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 2161 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt 2221 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 2281 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 2341 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgggggg atcctgtgcc 2401 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 2461 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 2521 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 2581 caatagcagg catgctgggg atgcggtggg ctctatggcg gccgcaggaa cccctagtga 2641 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg 2701 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc 2761 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 2821 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 2881 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt 2941 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 3001 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg 3061 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 3121 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc 3181 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga 3241 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg 3301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc 3361 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 3421 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 3481 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 3541 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 3601 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 3661 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 3721 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 3781 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 3841 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 3901 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 3961 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 4021 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 4081 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 4141 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 4201 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 4261 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 4321 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 4381 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 4441 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 4501 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 4561 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 4621 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 4681 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 4741 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 4801 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 4861 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 4921 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 4981 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 5041 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 5101 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 5161 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 5221 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 5281 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 5341 ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc tcactgaggc 5401 cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag 5461 agggagtggc caactccatc actaggggtt cct //