LOCUS       Exported               11264 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral vector to express wild-type N terminal HTT gene with 
            partial long 3'UTR in mammalian cells (encodes Myc-tagged wild-type 
            huntingtin exon1 fragment with 23 repeats of Q).
ACCESSION   .
VERSION     .
KEYWORDS    pUltra-Exon1Q23-Myc-B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11264)
  AUTHORS   Xu H, An JJ, Xu B
  TITLE     Distinct cellular toxicity of two mutant huntingtin mRNA variants 
            due to translation regulation.
  JOURNAL   PLoS One. 2017 May 11;12(5):e0177610. doi: 
            10.1371/journal.pone.0177610. eCollection 2017.
  PUBMED    28494017
REFERENCE   2  (bases 1 to 11264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11264
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1833..1950
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2015..3226
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3063..3082
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3596..3625
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3641..3658
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3686..3739
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    7104..7692
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7157..7177)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7575..7586)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7783..8016
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8088..8209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8125..8144)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8179..8198
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8249..8384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8311..8330
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8404..8423)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8405..8423)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8433..8450)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8433..8449)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8442..8464)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8591..9046
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8678..8697)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8888..8909
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9072..9176
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9177..10037
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9395..9414)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10208..10796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10697..10716
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10950..10967
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11084..11105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11120..11150
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11158..11174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11163..11185
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11182..11198
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11182..11198
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11217..11237
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11219..11237
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     1801 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     1861 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     1921 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     1981 gtttggttaa ttaacccgtg tcggctccag atctggcctc cgcgccgggt tttggcgcct
     2041 cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag
     2101 cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc
     2161 ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca
     2221 ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct
     2281 gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg
     2341 gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt
     2401 gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc
     2461 cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag
     2521 caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt
     2581 cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat
     2641 ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg
     2701 ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag
     2761 aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac
     2821 ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta
     2881 taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac
     2941 gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg
     3001 ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta
     3061 gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt
     3121 ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag
     3181 taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacgaag cttgggctgc
     3241 aggtccgatc caccgggatc cactagtcca gtgtggtgga attctgcaga taaacaccac
     3301 catggcgacc ctggaaaagc tgatgaaggc cttcgagtcc ctcaagtcct tccagcagca
     3361 gcagcagcaa cagcagcaac agcagcagca acaacagcag cagcagcaac aacaacagca
     3421 gccgccaccg ccgccgccgc cgccgccgcc tcctcagctt cctcagccgc cgccgcaggc
     3481 acagccgctg ctgcctcagc cgcagccgcc cccgccgccg cccccgccgc cacccggccc
     3541 ggctgtggct gaggagccgc tgcaccgacc agcggccgct cgaggtcacc cattcgaaca
     3601 aaaactcatc tcagaagagg atctgaatat gcataccggt catcatcacc atcaccattg
     3661 agtttctaga ggatccggct ccggcgaggg caggggaagt ctactaacat gcggggacgt
     3721 ggaggaaaat cccggcccag ctagcgtgtg atcagaattc atttaattat atcagtaaag
     3781 agattaattt taacgtaact ctttctatgc ccgtgtaaag tatgtgaatc gcaaggcctg
     3841 tgctgcatgc gacagcgtcc ggggtggtgg acagggcccc cggccacgct ccctctcctg
     3901 tagccactgg catagccctc ctgagcaccc gctgacattt ccgttgtaca tgttcctgtt
     3961 tatgcattca caaggtgact gggatgtaga gaggcgttag tgggcaggtg gccacagcag
     4021 gactgaggac aggcccccat tatcctaggg gtgcgctcac ctgcagcccc tcctcctcgg
     4081 gcacagacga ctgtcgttct ccacccacca gtcagggaca gcagcctccc tgtcactcag
     4141 ctgagaaggc cagccctccc tggctgtgag cagcctccac tgtgtccaga gacatgggcc
     4201 tcccactcct gttccttgct agccctgggg tggcgtctgc ctaggagctg gctggcaggt
     4261 gttgggacct gctgctccat ggatgcatgc cctaagagtg tcactgagct gtgttttgtc
     4321 tgagcctctc tcggtcaaca gcaaagcttg gtgtcttggc actgttagtg acagagccca
     4381 gcatcccttc tgcccccgtt ccagctgaca tcttgcacgg tgaccccttt tagtcaggag
     4441 agtgcagatc tgtgctcatc ggagactgcc ccacggccct gtcagagccg ccactcctat
     4501 ccccaggcca ggtccctgga ccagcctcct gtttgcaggc ccagaggagc caagtcatta
     4561 aaatggaggt ggattctgga tggccgggct gctgctgatg taggagctgg atttgggagc
     4621 tctgcttgct gactggctgt gagacgaggc aggggctctg cttcctcagc cctagaggcg
     4681 agccaggcaa ggttggcgac tgtcatgtgg cttggtttgg tcatgcccgt cgatgttttg
     4741 ggtattgaat gtggtaagtg gaggaaacgt tggaactctg tgcaggtgct gccttgagac
     4801 ccccaagctt ccacctgtcc ctctcctatg tggcagctgg ggagcagctg agatgtggac
     4861 ttgtatgctg cccacatacg tgagggggag ctgaaaggga gcccctcctc tgagcagcct
     4921 ctgccaggcc tgtatgaggc ttttcccacc agctcccaac agaggcctcc cccagccagg
     4981 accacctcgt cctcgtggcg gggcagcagg agcggtagaa aggggtccga tgtttgagga
     5041 ggcccttaag ggaagctact gaattataac acgtaagaaa atcaccattc ttccgtattg
     5101 gttgggggct cctgtttctc atcctagctt tttcctggaa agcccgctag aaggtttggg
     5161 aacgagggga aagttctcag aactgttggc tgctccccac ccgcctcccg cctcccccgc
     5221 aggttatgtc agcagctctg agacagcagt atcacaggcc agatgttgtt cctggctaga
     5281 tgtttacatt tgtaagaaat aacactgtga atgtaaaaca gagccattcc cttggaatgc
     5341 atatcgctgg gctcaacata gagtttgtct tcctcttgtt tacgacgtga tctaaaccag
     5401 tccttagcaa ggggctcaga acaccccgct ctggcagtag gtgtccccca cccccaaaga
     5461 cctgcctgtg tgctccggag atgaatatga gctcattagt aaaaatgact tcacccacgc
     5521 atatacataa agtatccatg catgtgcata tagacacatc tataatttta cacacacacc
     5581 tctcaagacg gagatgcatg gcctctaaga gtgcccgtgt cggttcttcc tggaagttga
     5641 ctttccttag acccgccagg tcaagttagc cgcgtgacgg acatccaggc gtgggacgtg
     5701 gtcagggcag ggctcattca ttacccacta ggatcccact ggcgaagatg gtctccatat
     5761 cagctctctg cagaagggag gaagacttta tcatgttcct aaaaatctgt ggcaagcacc
     5821 catcgtatta tccaaatttt gttgcaaatg tgattaattt ggttgtcaag ttttgggggt
     5881 gggctgtggg gagattgctt ttgttttcct gctggtaata tcgggaaaga ttttaatgaa
     5941 accagggtag aattgtttgg caatgcactg aagcgtgttt ctttcccaaa atgtgcctcc
     6001 cttccgctgc gggcccagct gtgtctatgt aggtgatgtt tccagctgcc aagtgctctt
     6061 tgttactgtc caccctcatt tctgccagcg catgtgtcct ttcaagggga aaatgtgaag
     6121 ctgaaccccc tccagacacc cagaatgtag catctgagaa ggccctgtgc cctaaaggac
     6181 acccctcgcc cccatcttca tggagggggt catttcagag ccctcggagc caatgaacag
     6241 ctcctcctct tggagctgag atgagcccca cgtggagctc gggacggata gtagacagca
     6301 ataactcggt gtgtggccgc ctggcaggtg gaacttcctc ccgttgcggg gtggagtgag
     6361 gttagttctg tgtgtctggt gggtggagtc aggcttctct tgctacctgt gagcatcctt
     6421 cccagcagac atcctcatcg ggctttgtcc ctcccccgct tcctccctct gcggggagga
     6481 cccgggacca cagctgctgg ccagggtaga cttggagctg tcctccagag gggtcacgtg
     6541 taggagtgag aagaaggaag atcttgagag ctgctgaggg accttggaga gctcaggatg
     6601 gctcagacga ggacactcgc ttgccgggcc tgggcctcct gggaaggagg gagctgctca
     6661 gaatgccgca tgacaactga aggcaacctg gaaggttcag gggccgctct tcccccatgt
     6721 gcctgtcacg ctctgatgca gtcaaaggaa cgccttcccc tcagttgttt ctaagagcag
     6781 agtctcccgc tgcaatctgg gtggtaactg ccagccttgg aggatcgtgg ccaacgtgga
     6841 cctgcctacg gagggtgggc tctgacccaa gtggggcctc cttgtccagg tctcactgct
     6901 ttgcaccgtg gtcagaggga ctgtcagctg agcttgagct cccctggagc cagcagggct
     6961 gtgatgggcg agtcccggag ccccacccag acctgaatgc ttctgagagc aaagggaagg
     7021 actgacgaga gatgtatatt taatttttta actgctgcaa acattgtaca tccaagaatt
     7081 cgttccggag tcgtcgactc gacaatcaac ctctggatta caaaatttgt gaaagattga
     7141 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7201 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7261 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7321 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7381 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7441 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     7501 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7561 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7621 ctctgcggcc tcttccgcat cttcgccttc gccctcagac gagtcggatc tccctttggg
     7681 ccgcctcccc gcctggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg
     7741 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctacgtaact
     7801 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     7861 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7921 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7981 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     8041 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     8101 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8161 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     8221 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8281 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     8341 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     8401 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     8461 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     8521 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     8581 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     8641 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     8701 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     8761 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     8821 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     8881 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     8941 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     9001 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     9061 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9121 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     9181 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     9241 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     9301 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     9361 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     9421 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     9481 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     9541 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     9601 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     9661 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     9721 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     9781 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     9841 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     9901 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     9961 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    10021 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    10081 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    10141 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    10201 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    10261 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    10321 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    10381 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    10441 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    10501 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    10561 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    10621 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    10681 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    10741 tctcacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    10801 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10861 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    10921 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    10981 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    11041 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    11101 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    11161 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
    11221 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaa
//
