LOCUS Exported 11952 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION For membrane localized optoDroplets. ACCESSION . VERSION . KEYWORDS Myristoylated optoDroplet SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11952) AUTHORS Dine E, Gil AA, Uribe G, Brangwynne CP, Toettcher JE TITLE Protein Phase Separation Provides Long-Term Memory of Transient Spatial Stimuli. JOURNAL Cell Syst. 2018 May 24. pii: S2405-4712(18)30190-X. doi: 10.1016/j.cels.2018.05.002. PUBMED 29859829 REFERENCE 2 (bases 1 to 11952) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11952 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2050..2167 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2320..2727 /label=SFFV promoter /note="spleen focus-forming virus long terminal repeat (LTR) promoter" CDS 2761..2781 /codon_start=1 /product="N-myristoylation signal from Src kinase (Pellman et al., 1985; Kaplan et al., 1988)" /label=myr /translation="MGSSKSK" CDS 3186..3197 /codon_start=1 /product="WELQut protease recognition and cleavage site" /label=WELQut site /translation="WELQ" CDS 3457..4152 /codon_start=1 /product="monomeric red fluorescent protein optimized for use as a fusion tag (Shemiakina et al., 2012)" /label=FusionRed /note="mammalian codon-optimized" /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY STGGAGDGGK" CDS 4171..5664 /codon_start=1 /product="N-terminal photolyase homology region of Arabidopsis thaliana cryptochrome 2" /label=CRY2PHR /note="binds to CIB1 upon exposure to blue light (Kennedy et al., 2010)" /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA" misc_feature 5816..6404 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5869..5889) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6287..6298) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6407..6423) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6408..6424) /label=pBluescriptKS /note="For pBluescript vector" LTR 6514..6747 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" promoter complement(6772..6790) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(6773..6790) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(7080..7099) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(7313..7332) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7432..7454 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(7492..7510) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7578..7682 /gene="bla" /label=AmpR promoter CDS 7683..8543 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7901..7920) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8714..9302 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9203..9222 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9456..9473 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(9543..9563) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 9548..9877 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9728..9863 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9790..9809 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 11011..11076 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 11206..11226 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 11651..11785 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(11688..11707) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 11742..11761 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt 2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca 2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat 2221 cgaattcctg cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga 2281 atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca 2341 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag 2401 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa 2461 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga 2521 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg 2581 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt 2641 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca 2701 gtcctccgac agactgagtc gcccgggggg gatctggagc tctcgagaat tctcacgcgt 2761 atgggttcct ccaaatccaa gcccaaggac gcatctcagc gacgtcgcgc ctcaaacgat 2821 tatacccaac aagcaaccca aagctatggg gcctacccca cccagcccgg gcagggctat 2881 tcccagcaga gcagtcagcc ctacggacag cagagttaca gtggttatag ccagtccacg 2941 gacacttcag gctatggcca gagcagctat tcttcttatg gccagagcca gaacacaggc 3001 tatggaactc agtcaactcc ccagggatat ggctcgactg gcggctatgg cagtagccag 3061 agctcccaat cgtcttacgg gcagcagtcc tcctatcctg gctatggcca gcagccagct 3121 cccagcagca cctcgggaag ttacggtagc agttctcaga gcagcagcta tgggcagccc 3181 cagagtggga gctacagcca gcagcctagc tatggtggac agcagcaaag ctatggacag 3241 cagcaaagct ataatccccc tcagggctat ggacagcaga accagtacaa cagcagcagt 3301 ggtggtggag gtggaggtgg aggtggaggt aactatggcc aagatcaatc ctccatgagt 3361 agtggtggtg gcagtggtgg cggttatggc aatcaagacc agagtggtgg aggtggcagc 3421 ggtggctatg gacagcagga ccgtggaggt ggaggaatgg tgagcgagct gattaaggag 3481 aacatgccca tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca 3541 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtcgtcgag 3601 ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaga 3661 accttcatca agcaccctcc gggcatcccc gacttcttta agcagtcctt ccctgagggc 3721 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac 3781 accagcctcc aggacggctg cctcatctac aacgtcaagg ttagaggggt gaacttccca 3841 gccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagacgatg 3901 taccccgctg acggcggcct ggaaggcgca tgtgacatgg ccctgaagct cgtgggcggg 3961 ggccacctga tctgcaacct tgagaccaca tacagatcca agaaacccgc tacgaacctc 4021 aagatgcccg gcgtctacaa cgtggaccac agactggaaa gaatcaagga ggccgacgat 4081 gagacctacg tcgagcagca cgaggtggct gtggccagat actctactgg tggcgctggt 4141 gatggaggta aagcggccac gcgtacgcgt atgaagatgg acaaaaagac tatagtttgg 4201 tttagaagag acctaaggat tgaggataat cctgcattag cagcagctgc tcacgaagga 4261 tctgtttttc ctgtcttcat ttggtgtcct gaagaagaag gacagtttta tcctggaaga 4321 gcttcaagat ggtggatgaa acaatcactt gctcacttat ctcaatcctt gaaggctctt 4381 ggatctgacc tcactttaat caaaacccac aacacgattt cagcgatctt ggattgtatc 4441 cgcgttaccg gtgctacaaa agtcgtcttt aaccacctct atgatcctgt ttcgttagtt 4501 cgggaccata ccgtaaagga gaagctggtg gaacgtggga tctctgtgca aagctacaat 4561 ggagatctat tgtatgaacc gtgggagata tactgcgaaa agggcaaacc ttttacgagt 4621 ttcaattctt actggaagaa atgcttagat atgtcgattg aatccgttat gcttcctcct 4681 ccttggcggt tgatgccaat aactgcagcg gctgaagcga tttgggcgtg ttcgattgaa 4741 gaactagggc tggagaatga ggccgagaaa ccgagcaatg cgttgttaac tagagcttgg 4801 tctccaggat ggagcaatgc tgataagtta ctaaatgagt tcatcgagaa gcagttgata 4861 gattatgcaa agaacagcaa gaaagttgtt gggaattcta cttcactact ttctccgtat 4921 ctccatttcg gggaaataag cgtcagacac gttttccagt gtgcccggat gaaacaaatt 4981 atatgggcaa gagataagaa cagtgaagga gaagaaagtg cagatctttt tcttagggga 5041 atcggtttaa gagagtattc tcggtatata tgtttcaact tcccgtttac tcacgagcaa 5101 tcgttgttga gtcatcttcg gtttttccct tgggatgctg atgttgataa gttcaaggcc 5161 tggagacaag gcaggaccgg ttatccgttg gtggatgccg gaatgagaga gctttgggct 5221 accggatgga tgcataacag aataagagtg attgtttcaa gctttgctgt gaagtttctt 5281 ctccttccat ggaaatgggg aatgaagtat ttctgggata cacttttgga tgctgatttg 5341 gaatgtgaca tccttggctg gcagtatatc tctgggagta tccccgatgg ccacgagctt 5401 gatcgcttgg acaatcccgc gttacaaggc gccaaatatg acccagaagg tgagtacata 5461 aggcaatggc ttcccgagct tgcgagattg ccaactgaat ggatccatca tccatgggac 5521 gctcctttaa ccgtactcaa agcttctggt gtggaactcg gaacaaacta tgcgaaaccc 5581 attgtagaca tcgacacagc tcgtgagcta ctagctaaag ctatttcaag aacccgtgaa 5641 gcacagatca tgatcggagc agcagcccgg gatccaccgg tcgccaccgg ttcagggagc 5701 ggatccggct cagggagtgc ggccgcaact cccacctgca acatgcgtga ctgactgagg 5761 ccgcgactct agagtcgacc tgcaggcatg caagcttgat atcaagctta tcgataatca 5821 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 5881 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 5941 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 6001 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 6061 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 6121 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 6181 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg 6241 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 6301 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 6361 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc 6421 tcgagggaat taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag 6481 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 6541 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg 6601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 6661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 6721 tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg 6781 tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa 6841 cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc 6901 tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa 6961 cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat 7021 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc 7081 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc 7141 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc 7201 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg 7261 cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct 7321 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct 7381 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 7441 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga 7501 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 7561 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 7621 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 7681 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 7741 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 7801 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 7861 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 7921 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 7981 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 8041 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 8101 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 8161 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 8221 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 8281 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 8341 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 8401 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 8461 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 8521 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 8581 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 8641 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 8701 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 8761 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 8821 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 8881 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 8941 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 9001 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 9061 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 9121 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 9181 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 9241 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 9301 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 9361 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 9421 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 9481 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 9541 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 9601 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 9661 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 9721 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 9781 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 9841 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt 9901 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag 9961 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac 10021 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga 10081 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc 10141 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc 10201 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag 10261 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga 10321 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga 10381 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt 10441 tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg 10501 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg 10561 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg 10621 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg 10681 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc 10741 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc 10801 gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg 10861 aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt 10921 atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac 10981 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt 11041 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa 11101 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca 11161 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga 11221 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg 11281 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg 11341 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat 11401 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac 11461 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat 11521 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt 11581 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 11641 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 11701 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 11761 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa 11821 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac 11881 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac 11941 aaacttagta gt //