LOCUS       Exported               21838 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  contains the AAV3B cap genes, AAV2 rep genes, and also adenovirus 
            helper genes.
ACCESSION   .
VERSION     .
KEYWORDS    pDGM3B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21838)
  AUTHORS   Khan IF, Hirata RK, Russell DW
  TITLE     AAV-mediated gene targeting methods for human cells.
  JOURNAL   Nat Protoc. 2011 Apr;6(4):482-501. doi: 10.1038/nprot.2011.301. Epub
            2011 Mar 24.
  PUBMED    21455185
REFERENCE   2  (bases 1 to 21838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..21838
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   21..123
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     211..361
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     primer_bind     483..499
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     483..499
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             5277..5288
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      7510..7519
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     repeat_region   19735..19837
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      complement(20028..20616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(20108..20127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(20768..21628)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     21391..21410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(21629..21733)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     21801..21819
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggatccgaat tcttaattaa catcatcaat aatatacctt attttggatt gaagccaata
       61 tgataatgag ggggtggagt ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg
      121 tagtagtgtg gcggaagtgt gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg
      181 tggcaaaagt gacgtttttg gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg
      241 gttttaggcg gatgttgtag taaatttggg cgtaaccgag taagatttgg ccattttcgc
      301 gggaaaactg aataagagga agtgaaatct gaataatttt gtgttactca tagcgcgtaa
      361 tatttgtcta gggccgcggg gactttgacc gtttacgtgg agactcgccc aggtgttttt
      421 ctcaggtgtt ttccgcgttc cgggtcaaag ttggcgtttt attattatag tcagggggat
      481 cctctagaac tagtggatcc gtccctagaa gtaaaaaagg gaaaaaagag tgtgtttgtc
      541 aaaataggag acaggtggtg gcaaccaggg acttataggg gaccttacat ctacagacca
      601 acagatgccc ccttaccata tacaggaaga tatgacttaa attgggatag gtgggtcaca
      661 atcaacggct ataaagtgtt atacagatcc ctcccctttc gtgaaagact cgccagagct
      721 agacctcctt ggtgtatgct aactgagaaa gagaaagacg acatgaaaca acaggtacat
      781 gattatattt atctaggaac aggaatgcac ttttggggaa aggttttcca taccaaggaa
      841 ggggcagtgg ctggactgat agaacattat tctgcaaaaa cttatggtat gagttattat
      901 gattagcctt tatttgccca accttgcggt tcccagggtt taaataagtt tatggttaca
      961 aactgttctt aaaacaagga tgtgagacaa gtggtttcct gagttggttt ggtatcaaat
     1021 gttctgatct gagctcttag tgttctattt tcctatgttc ttttggaatc tatccaagtc
     1081 ttatgtaaat gcttatgtaa accataatat aaaagagtgc tgattttttg agtaaacttg
     1141 caacagtcct aacattcttc tctcgtgtgt ttgtgtctgt tcgccatccc gtctccgctc
     1201 gtcacttatc cttcactttt cagagggtcc ccccgcagat cccggtcacc ctcaggtcgg
     1261 gacctgcaga agacgcccga gtgagcacgc agggtctcca ttttgaagcg ggaggtttga
     1321 acgcgcagcc gccatgccgg ggttttacga gattgtgatt aaggtcccca gcgaccttga
     1381 cgagcatctg cccggcattt ctgacagctt tgtgaactgg gtggccgaga aggaatggga
     1441 gttgccgcca gattctgaca tggatctgaa tctgattgag caggcacccc tgaccgtggc
     1501 cgagaagctg cagcgcgact ttctgacgga atggcgccgt gtgagtaagg ccccggaggc
     1561 ccttttcttt gtgcaatttg agaagggaga gagctacttc cacatgcacg tgctcgtgga
     1621 aaccaccggg gtgaaatcca tggttttggg acgtttcctg agtcagattc gcgaaaaact
     1681 gattcagaga atttaccgcg ggatcgagcc gactttgcca aactggttcg cggtcacaaa
     1741 gaccagaaat ggcgccggag gcgggaacaa ggtggtggat gagtgctaca tccccaatta
     1801 cttgctcccc aaaacccagc ctgagctcca gtgggcgtgg actaatatgg aacagtattt
     1861 aagcgcctgt ttgaatctca cggagcgtaa acggttggtg gcgcagcatc tgacgcacgt
     1921 gtcgcagacg caggagcaga acaaagagaa tcagaatccc aattctgatg cgccggtgat
     1981 cagatcaaaa acttcagcca ggtacatgga gctggtcggg tggctcgtgg acaaggggat
     2041 tacctcggag aagcagtgga tccaggagga ccaggcctca tacatctcct tcaatgcggc
     2101 ctccaactcg cggtcccaaa tcaaggctgc cttggacaat gcgggaaaga ttatgagcct
     2161 gactaaaacc gcccccgact acctggtggg ccagcagccc gtggaggaca tttccagcaa
     2221 tcggatttat aaaattttgg aactaaacgg gtacgatccc caatatgcgg cttccgtctt
     2281 tctgggatgg gccacgaaaa agttcggcaa gaggaacacc atctggctgt ttgggcctgc
     2341 aactaccggg aagaccaaca tcgcggaggc catagcccac actgtgccct tctacgggtg
     2401 cgtaaactgg accaatgaga actttccctt caacgactgt gtcgacaaga tggtgatctg
     2461 gtgggaggag ggcaagatga cggccaaggt cgtggagagc gccaaggcca ttctgggcgg
     2521 aagcaaggtg cgcgtggacc aaaagtgcaa gtcatcggcc cagatcgaac ccactcccgt
     2581 gatcgtcacc tccaacacca acatgtgcgc cgtgattgac gggaacagca ccaccttcga
     2641 gcatcagcag ccgctgcagg accggatgtt taaatttgaa cttacccgcc gtttggacca
     2701 tgactttggg aaggtcacca aacaggaagt aaaggacttt ttccggtggg cttccgatca
     2761 cgtgactgac gtggctcatg agttctacgt cagaaagggt ggagctaaga aacgccccgc
     2821 ctccaatgac gcggatgtaa gcgagccaaa acggcagtgc acgtcacttg cgcagccgac
     2881 aacgtcagac gcggaagcac cggcggacta cgcggacagg taccaaaaca aatgttctcg
     2941 tcacgtgggc atgaatctga tgctttttcc ctgtaaaaca tgcgagagaa tgaatcaaat
     3001 ttccaatgtc tgttttacgc atggtcaaag agactgtggg gaatgcttcc ctggaatgtc
     3061 agaatctcaa cccgtttctg tcgtcaaaaa gaagacttat cagaaactgt gtccaattca
     3121 tcatatcctg ggaagggcac ccgagattgc ctgttcggcc tgcgatttgg ccaatgtgga
     3181 cttggatgac tgtgtttctg agcaataaat gacttaaacc aggtatggct gctgacggtt
     3241 atcttccaga ttggctcgag gacaaccttt ctgaaggcat tcgtgagtgg tgggctctga
     3301 aacctggagt ccctcaaccc aaagcgaacc aacaacacca ggacaaccgt cggggtcttg
     3361 tgcttccggg ttacaaatac ctcggacccg gtaacggact cgacaaagga gagccggtca
     3421 acgaggcgga cgcggcagcc ctcgaacacg acaaagctta cgaccagcag ctcaaggccg
     3481 gtgacaaccc gtacctcaag tacaaccacg ccgacgccga gtttcaggag cgtcttcaag
     3541 aagatacgtc ttttgggggc aaccttggca gagcagtctt ccaggccaaa aagaggatcc
     3601 ttgagcctct tggtctggtt gaggaagcag ctaaaacggc tcctggaaag aagaggcctg
     3661 tagatcagtc tcctcaggaa ccggactcat catctggtgt tggcaaatcg ggcaaacagc
     3721 ctgccagaaa aagactaaat ttcggtcaga ctggcgactc agagtcagtc ccagaccctc
     3781 aacctctcgg agaaccacca gcagccccca caagtttggg atctaataca atggcttcag
     3841 gcggtggcgc accaatggca gacaataacg agggtgccga tggagtgggt aattcctcag
     3901 gaaattggca ttgcgattcc caatggctgg gcgacagagt catcaccacc agcaccagaa
     3961 cctgggccct gcccacttac aacaaccatc tctacaagca aatctccagc caatcaggag
     4021 cttcaaacga caaccactac tttggctaca gcaccccttg ggggtatttt gactttaaca
     4081 gattccactg ccacttctca ccacgtgact ggcagcgact cattaacaac aactggggat
     4141 tccggcccaa gaaactcagc ttcaagctct tcaacatcca agttaaagag gtcacgcaga
     4201 acgatggcac gacgactatt gccaataacc ttaccagcac ggttcaagtg tttacggact
     4261 cggagtatca gctcccgtac gtgctcgggt cggcgcacca aggctgtctc ccgccgtttc
     4321 cagcggacgt cttcatggtc cctcagtatg gatacctcac cctgaacaac ggaagtcaag
     4381 cggtgggacg ctcatccttt tactgcctgg agtacttccc ttcgcagatg ctaaggactg
     4441 gaaataactt ccaattcagc tataccttcg aggatgtacc ttttcacagc agctacgctc
     4501 acagccagag tttggatcgc ttgatgaatc ctcttattga tcagtatctg tactacctga
     4561 acagaacgca aggaacaacc tctggaacaa ccaaccaatc acggctgctt tttagccagg
     4621 ctgggcctca gtctatgtct ttgcaggcca gaaattggct acctgggccc tgctaccggc
     4681 aacagagact ttcaaagact gctaacgaca acaacaacag taactttcct tggacagcgg
     4741 ccagcaaata tcatctcaat ggccgcgact cgctggtgaa tccaggacca gctatggcca
     4801 gtcacaagga cgatgaagaa aaatttttcc ctatgcacgg caatctaata tttggcaaag
     4861 aagggacaac ggcaagtaac gcagaattag ataatgtaat gattacggat gaagaagaga
     4921 ttcgtaccac caatcctgtg gcaacagagc agtatggaac tgtggcaaat aacttgcaga
     4981 gctcaaatac agctcccacg actagaactg tcaatgatca gggggcctta cctggcatgg
     5041 tgtggcaaga tcgtgacgtg taccttcaag gacctatctg ggcaaagatt cctcacacgg
     5101 atggacactt tcatccttct cctctgatgg gaggctttgg actgaaacat ccgcctcctc
     5161 aaatcatgat caaaaatact ccggtaccgg caaatcctcc gacgactttc agcccggcca
     5221 agtttgcttc atttatcact cagtactcca ctggacaggt cagcgtggaa attgagtggg
     5281 agctacagaa agaaaacagc aaacgttgga atccagagat tcagtacact tccaactaca
     5341 acaagtctgt taatgtggac tttactgtag acactaatgg tgtttatagt gaacctcgcc
     5401 ctattggaac ccggtatctc acacgaaact tgtaatcctg gttaatcaat aaaccgttta
     5461 attcgtttca gttgaacttt ggctcttgtg cacttcttat cttatcttgt ttccatggct
     5521 atcgatgcat gtccttgggt ccggcctgct gaatgcgcag gcggtcggcc atgccccagg
     5581 cttcgttttg acatcggcgc aggtctttgt agtagtcttg catgagcctt tctaccggca
     5641 cttcttcttc tccttcctct tgtcctgcat ctcttgcatc tatcgctgcg gcggcggcgg
     5701 agtttggccg taggtggcgc cctcttcctc ccatcgtgtg accccgaagc ccctcatcgg
     5761 ctgaagcagg gctaggtcgg cgacaacgcg ctcggctaat atggcctgct gcacctgcgt
     5821 gagggtagac tggaagtcat ccatgtccac aaagcggtgg tatgcgcccg tgttgatggt
     5881 gtaagtgcag ttggccataa cggaccagtt aacggtctgg tgacccggct gcgagagctc
     5941 ggtgtacctg agacgcgagt aagccctcga gtcaaatacg tagtcgttac aagtccgcac
     6001 caggtactgg tatcccacca aaaagtgcgg cggcggctgg cggtagaggg gccagcgtag
     6061 ggtggccggg gctccggggg cgagatcttc caacataagg cgatgatatc cgtagatgta
     6121 cctggacatc caggtgatgc cggcggcggt ggtggaggcg cgcggaaagt cgcggacgcg
     6181 gttccagatg ttgcgcagcg gcaaaaagtg ctccatggtc gggacgctct ggccggtcag
     6241 gcgcgcgcaa tcgttgacgc tctagaccgt gcaaaaggag agcctgtaag cgggcactct
     6301 tccgtggtct ggtggataaa ttcgcaaggg tatcatggcg gacgaccggg gttcgagccc
     6361 cgtatccggc cgtccgccgt gatccatgcg gttaccgccc gcgtgtcgaa cccaggtgtg
     6421 cgacgtcaga caacggggga gtgctccttt tggcttcctt ccaggcgcgg cggctgctgc
     6481 gctagctttt ttggccactg gccgcgcgca gcgtaagcgg ttaggctgga aagcgaaagc
     6541 attaagtggc tcgctccctg tagccggagg gttattttcc aagggttgag tcgcgggacc
     6601 cccggttcga gtctcggacc ggccggactg cggcgaacgg gggtttgtct ccccgtcatg
     6661 caagaccccg cttgcaaatt cctccggaaa cagggacgag cccctttttt gcttttccca
     6721 gatgcatccg gtgctgcggc agatgcgccc ccctcctcag cagcggcaag agcaagagca
     6781 gcggcagaca tgcagggcac cctcccctcc tcctaccgcg tcaggagggg cgacatccgc
     6841 ggttgacgcg gcagcagatg gtgattacga acccccgcgg cgccgggccc ggcactacct
     6901 ggacttggag gagggcgagg gcctggcgcg gctaggagcg ccctctcctg agcggcaccc
     6961 aagggtgcag ctgaagcgtg atacgcgtga ggcgtacgtg ccgcggcaga acctgtttcg
     7021 cgaccgcgag ggagaggagc ccgaggagat gcgggatcga aagttccacg cagggcgcga
     7081 gctgcggcat ggcctgaatc gcgagcggtt gctgcgcgag gaggactttg agcccgacgc
     7141 gcgaaccggg attagtcccg cgcgcgcaca cgtggcggcc gccgacctgg taaccgcata
     7201 cgagcagacg gtgaaccagg gcgatcgcac cctttggcgc atcccattct ccagtaactt
     7261 tatgtccatg ggcgcactca cagacctggg ccaaaacctt ctctacgcca actccgccca
     7321 cgcgctagac atgacttttg aggtggatcc catggacgag cccacccttc tttatgtttt
     7381 gtttgaagtc tttgacgtgg tccgtgtgca ccagccgcac cgcggcgtca tcgaaaccgt
     7441 gtacctgcgc acgcccttct cggccggcaa cgccacaaca taaagaagca agcaacatca
     7501 acaacagctg ccgccatggg ctccagtgag caggaactga aagccattgt caaagatctt
     7561 ggttgtgggc catatttttt gggcacctat gacaagcgct ttccaggctt tgtttctcca
     7621 cacaagctcg cctgcgccat agtcaatacg gccggtcgcg agactggggg cgtacactgg
     7681 atggcctttg cctggaaccc gcactcaaaa acatgctacc tctttgagcc ctttggcttt
     7741 tctgaccagc gactcaagca ggtttaccag tttgagtacg agtcactcct gcgccgtagc
     7801 gccattgctt cttcccccga ccgctgtata acgctggaaa agtccaccca aagcgtacag
     7861 gggcccaact cggccgcctg tggactattc tgctgcatgt ttctccacgc ctttgccaac
     7921 tggccccaaa ctcccatgga tcacaacccc accatgaacc ttattaccgg ggtacccaac
     7981 tccatgctca acagtcccca ggtacagccc accctgcgtc gcaaccagga acagctctac
     8041 agcttcctgg agcgccactc gccctacttc cgcagccaca gtgcgcagat taggagcgcc
     8101 acttcttttt gtcacttgaa aaacatgtaa aaataatgta ctagagacac tttcaataaa
     8161 ggcaaatgct tttatttgta cactctcggg tgattattta cccccaccct tgccgtctgc
     8221 gccgtttaaa aatcaaaggg gttctgccgc gcatcgctat gcgccactgg cagggacacg
     8281 ttgcgatact ggtgtttagt gctccactta aactcaggca caaccatccg cggcagctcg
     8341 gtgaagtttt cactccacag gctgcgcacc atcaccaacg cgtttagcag gtcgggcgcc
     8401 gatatcttga agtcgcagtt ggggcctccg ccctgcgcgc gcgagttgcg atacacaggg
     8461 ttgcagcact ggaacactat cagcgccggg tggtgcacgc tggccagcac gctcttgtcg
     8521 gagatcagat ccgcgtccag gtcctccgcg ttgctcaggg cgaacggagt caactttggt
     8581 agctgccttc ccaaaaaggg cgcgtgccca ggctttgagt tgcactcgca ccgtagtggc
     8641 atcaaaaggt gaccgtgccc ggtctgggcg ttaggataca gcgcctgcat aaaagccttg
     8701 atctgcttaa aagccacctg agcctttgcg ccttcagaga agaacatgcc gcaagacttg
     8761 ccggaaaact gattggccgg acaggccgcg tcgtgcacgc agcaccttgc gtcggtgttg
     8821 gagatctgca ccacatttcg gccccaccgg ttcttcacga tcttggcctt gctagactgc
     8881 tccttcagcg cgcgctgccc gttttcgctc gtcacatcca tttcaatcac gtgctcctta
     8941 tttatcataa tgcttccgtg tagacactta agctcgcctt cgatctcagc gcagcggtgc
     9001 agccacaacg cgcagcccgt gggctcgtga tgcttgtagg tcacctctgc aaacgactgc
     9061 aggtacgcct gcaggaatcg ccccatcatc gtcacaaagg tcttgttgct ggtgaaggtc
     9121 agctgcaacc cgcggtgctc ctcgttcagc caggtcttgc atacggccgc cagagcttcc
     9181 acttggtcag gcagtagttt gaagttcgcc tttagatcgt tatccacgtg gtacttgtcc
     9241 atcagcgcgc gcgcagcctc catgcccttc tcccacgcag acacgatcgg cacactcagc
     9301 gggttcatca ccgtaatttc actttccgct tcgctgggct cttcctcttc ctcttgcgtc
     9361 cgcataccac gcgccactgg gtcgtcttca ttcagccgcc gcactgtgcg cttacctcct
     9421 ttgccatgct tgattagcac cggtgggttg ctgaaaccca ccatttgtag cgccacatct
     9481 tctctttctt cctcgctgtc cacgattacc tctggtgatg gcgggcgctc gggcttggga
     9541 gaagggcgct tctttttctt cttgggcgca atggccaaat ccgccgccga ggtcgatggc
     9601 cgcgggctgg gtgtgcgcgg caccagcgcg tcttgtgatg agtcttcctc gtcctcggac
     9661 tcgatacgcc gcctcatccg cttttttggg ggcgcccggg gaggcggcgg cgacggggac
     9721 ggggacgaca cgtcctccat ggttggggga cgtcgcgccg caccgcgtcc gcgctcgggg
     9781 gtggtttcgc gctgctcctc ttcccgactg gccatttcct tctcctatag gcagaaaaag
     9841 atcatggagt cagtcgagaa gaaggacagc ctaaccgccc cctctgagtt cgccaccacc
     9901 gcctccaccg atgccgccaa cgcgcctacc accttccccg tcgaggcacc cccgcttgag
     9961 gaggaggaag tgattatcga gcaggaccca ggttttgtaa gcgaagacga cgaggaccgc
    10021 tcagtaccaa cagaggataa aaagcaagac caggacaacg cagaggcaaa cgaggaacaa
    10081 gtcgggcggg gggacgaaag gcatggcgac tacctagatg tgggagacga cgtgctgttg
    10141 aagcatctgc agcgccagtg cgccattatc tgcgacgcgt tgcaagagcg cagcgatgtg
    10201 cccctcgcca tagcggatgt cagccttgcc tacgaacgcc acctattctc accgcgcgta
    10261 ccccccaaac gccaagaaaa cggcacatgc gagcccaacc cgcgcctcaa cttctacccc
    10321 gtatttgccg tgccagaggt gcttgccacc tatcacatct ttttccaaaa ctgcaagata
    10381 cccctatcct gccgtgccaa ccgcagccga gcggacaagc agctggcctt gcggcagggc
    10441 gctgtcatac ctgatatcgc ctcgctcaac gaagtgccaa aaatctttga gggtcttgga
    10501 cgcgacgaga agcgcgcggc aaacgctctg caacaggaaa acagcgaaaa tgaaagtcac
    10561 tctggagtgt tggtggaact cgagggtgac aacgcgcgcc tagccgtact aaaacgcagc
    10621 atcgaggtca cccactttgc ctacccggca cttaacctac cccccaaggt catgagcaca
    10681 gtcatgagtg agctgatcgt gcgccgtgcg cagcccctgg agagggatgc aaatttgcaa
    10741 gaacaaacag aggagggcct acccgcagtt ggcgacgagc agctagcgcg ctggcttcaa
    10801 acgcgcgagc ctgccgactt ggaggagcga cgcaaactaa tgatggccgc agtgctcgtt
    10861 accgtggagc ttgagtgcat gcagcggttc tttgctgacc cggagatgca gcgcaagcta
    10921 gaggaaacat tgcactacac ctttcgacag ggctacgtac gccaggcctg caagatctcc
    10981 aacgtggagc tctgcaacct ggtctcctac cttggaattt tgcacgaaaa ccgccttggg
    11041 caaaacgtgc ttcattccac gctcaagggc gaggcgcgcc gcgactacgt ccgcgactgc
    11101 gtttacttat ttctatgcta cacctggcag acggccatgg gcgtttggca gcagtgcttg
    11161 gaggagtgca acctcaagga gctgcagaaa ctgctaaagc aaaacttgaa ggacctatgg
    11221 acggccttca acgagcgctc cgtggccgcg cacctggcgg acatcatttt ccccgaacgc
    11281 ctgcttaaaa ccctgcaaca gggtctgcca gacttcacca gtcaaagcat gttgcagaac
    11341 tttaggaact ttatcctaga gcgctcagga atcttgcccg ccacctgctg tgcacttcct
    11401 agcgactttg tgcccattaa gtaccgcgaa tgccctccgc cgctttgggg ccactgctac
    11461 cttctgcagc tagccaacta ccttgcctac cactctgaca taatggaaga cgtgagcggt
    11521 gacggtctac tggagtgtca ctgtcgctgc aacctatgca ccccgcaccg ctccctggtt
    11581 tgcaattcgc agctgcttaa cgaaagtcaa attatcggta cctttgagct gcagggtccc
    11641 tcgcctgacg aaaagtccgc ggctccgggg ttgaaactca ctccggggct gtggacgtcg
    11701 gcttaccttc gcaaatttgt acctgaggac taccacgccc acgagattag gttctacgaa
    11761 gaccaatccc gcccgcctaa tgcggagctt accgcctgcg tcattaccca gggccacatt
    11821 cttggccaat tgcaagccat caacaaagcc cgccaagagt ttctgctacg aaagggacgg
    11881 ggggtttact tggaccccca gtccggcgag gagctcaacc caatcccccc gccgccgcag
    11941 ccctatcagc agcagccgcg ggcccttgct tcccaggatg gcacccaaaa agaagctgca
    12001 gctgccgccg ccacccacgg acgaggagga atactgggac agtcaggcag aggaggtttt
    12061 ggacgaggag gaggaggaca tgatggaaga ctgggagagc ctagacgagg aagcttccga
    12121 ggtcgaagag gtgtcagacg aaacaccgtc accctcggtc gcattcccct cgccggcgcc
    12181 ccagaaatcg gcaaccggtt ccagcatggc tacaacctcc gctcctcagg cgccgccggc
    12241 actgcccgtt cgccgaccca accgtagatg ggacaccact ggaaccaggg ccggtaagtc
    12301 caagcagccg ccgccgttag cccaagagca acaacagcgc caaggctacc gctcatggcg
    12361 cgggcacaag aacgccatag ttgcttgctt gcaagactgt gggggcaaca tctccttcgc
    12421 ccgccgcttt cttctctacc atcacggcgt ggccttcccc cgtaacatcc tgcattacta
    12481 ccgtcatctc tacagcccat actgcaccgg cggcagcggc agcaacagca gcggccacac
    12541 agaagcaaag gcgaccggat agcaagactc tgacaaagcc caagaaatcc acagcggcgg
    12601 cagcagcagg aggaggagcg ctgcgtctgg cgcccaacga acccgtatcg acccgcgagc
    12661 ttagaaacag gatttttccc actctgtatg ctatatttca acagagcagg ggccaagaac
    12721 aagagctgaa aataaaaaac aggtctctgc gatccctcac ccgcagctgc ctgtatcaca
    12781 aaagcgaaga tcagcttcgg cgcacgctgg aagacgcgga ggctctcttc agtaaatact
    12841 gcgcgctgac tcttaaggac tagtttcgcg ccctttctca aatttaagcg cgaaaactac
    12901 gtcatctcca gcggccacac ccggcgccag cacctgttgt cagcgccatt atgagcaagg
    12961 aaattcccac gccctacatg tggagttacc agccacaaat gggacttgcg gctggagctg
    13021 cccaagacta ctcaacccga ataaactaca tgagcgcggg accccacatg atatcccggg
    13081 tcaacggaat acgcgcccac cgaaaccgaa ttctcctgga acaggcggct attaccacca
    13141 cacctcgtaa taaccttaat ccccgtagtt ggcccgctgc cctggtgtac caggaaagtc
    13201 ctgctcccac cactgtggta cttcccagag acgcccaggc cgaagttcag atgactaact
    13261 caggggcgca gcttgcgggc ggctttcgtc acagggtgcg gtcgcccggg cagggtataa
    13321 ctcacctgac aatcagaggg cgaggtattc agctcaacga cgagtcggtg agctcctcgc
    13381 ttggtctccg tccggacggg acatttcaga tcggcggcgc cggccgctct tcattcacgc
    13441 ctcgtcaggc aatcctaact ctgcagacct cgtcctctga gccgcgctct ggaggcattg
    13501 gaactctgca atttattgag gagtttgtgc catcggtcta ctttaacccc ttctcgggac
    13561 ctcccggcca ctatccggat caatttattc ctaactttga cgcggtaaag gactcggcgg
    13621 acggctacga ctgaatgtta agtggagagg cagagcaact gcgcctgaaa cacctggtcc
    13681 actgtcgccg ccacaagtgc tttgcccgcg actccggtga gttttgctac tttgaattgc
    13741 ccgaggatca tatcgagggc ccggcgcacg gcgtccggct taccgcccag ggagagcttg
    13801 cccgtagcct gattcgggag tttacccagc gccccctgct agttgagcgg gacaggggac
    13861 cctgtgttct cactgtgatt tgcaactgtc ctaaccctgg attacatcaa gatctttgtt
    13921 gccatctctg tgctgagtat aataaataca gaaattaaaa tatactgggg ctcctatcgc
    13981 catcctgtaa acgccaccgt cttcacccgc ccaagcaaac caaggcgaac cttacctggt
    14041 acttttaaca tctctccctc tgtgatttac aacagtttca acccagacgg agtgagtcta
    14101 cgagagaacc tctccgagct cagctactcc atcagaaaaa acaccaccct ccttacctgc
    14161 cgggaacgta cgagtgcgtc accggccgct gcaccacacc taccgcctga ccgtaaacca
    14221 gactttttcc ggacagacct caataactct gtttaccaga acaggaggtg agcttagaaa
    14281 acccttaggg tattaggcca aaggcgcagc tactgtgggg tttatgaaca attcaagcaa
    14341 ctctacgggc tattctaatt caggtttctc tagaaatgga cggaattatt acagagcagc
    14401 gcctgctaga aagacgcagg gcagcggccg agcaacagcg catgaatcaa gagctccaag
    14461 acatggttaa cttgcaccag tgcaaaaggg gtatcttttg tctggtaaag caggccaaag
    14521 tcacctacga cagtaatacc accggacacc gccttagcta caagttgcca accaagcgtc
    14581 agaaattggt ggtcatggtg ggagaaaagc ccattaccat aactcagcac tcggtagaaa
    14641 ccgaaggctg cattcactca ccttgtcaag gacctgagga tctctgcacc cttattaaga
    14701 ccctgtgcgg tctcaaagat cttattccct ttaactaata aaaaaaaata ataaagcatc
    14761 acttacttaa aatcagttag caaatttctg tccagtttat tcagcagcac ctccttgccc
    14821 tcctcccagc tctggtattg cagcttcctc ctggctgcaa actttctcca caatctaaat
    14881 ggaatgtcag tttcctcctg ttcctgtcca tccgcaccca ctatcttcat gttgttgcag
    14941 atgaagcgcg caagaccgtc tgaagatacc ttcaaccccg tgtatccata tgacacggaa
    15001 accggtcctc caactgtgcc ttttcttact cctccctttg tatcccccaa tgggtttcaa
    15061 gagagtcccc ctggggtact ctctttgcgc ctatccgaac ctctagttac ctccaatggc
    15121 atgcttgcgc tcaaaatggg caacggcctc tctctggacg aggccggcaa ccttacctcc
    15181 caaaatgtaa ccactgtgag cccacctctc aaaaaaacca agtcaaacat aaacctggaa
    15241 atatctgcac ccctcacagt tacctcagaa gccctaactg tggctgccgc cgcacctcta
    15301 atggtcgcgg gcaacacact caccatgcaa tcacaggccc cgctaaccgt gcacgactcc
    15361 aaacttagca ttgccaccca aggacccctc acagtgtcag aaggaaagct agccctgcaa
    15421 acatcaggcc ccctcaccac caccgatagc agtaccctta ctatcactgc ctcaccccct
    15481 ctaactactg ccactggtag cttgggcatt gacttgaaag agcccattta tacacaaaat
    15541 ggaaaactag gactaaagta cggggctcct ttgcatgtaa cagacgacct aaacactttg
    15601 accgtagcaa ctggtccagg tgtgactatt aataatactt ccttgcaaac taaagttact
    15661 ggagccttgg gttttgattc acaaggcaat atgcaactta atgtagcagg aggactaagg
    15721 attgattctc aaaacagacg ccttatactt gatgttagtt atccgtttga tgctcaaaac
    15781 caactaaatc taagactagg acagggccct ctttttataa actcagccca caacttggat
    15841 attaactaca acaaaggcct ttacttgttt acagcttcaa acaattccaa aaagcttgag
    15901 gttaacctaa gcactgccaa ggggttgatg tttgacgcta cagccatagc cattaatgca
    15961 ggagatgggc ttgaatttgg ttcacctaat gcaccaaaca caaatcccct caaaacaaaa
    16021 attggccatg gcctagaatt tgattcaaac aaggctatgg ttcctaaact aggaactggc
    16081 cttagttttg acagcacagg tgccattaca gtaggaaaca aaaataatga taagctaact
    16141 ttgtggacca caccagctcc atctcctaac tgtagactaa atgcagagaa agatgctaaa
    16201 ctcactttgg tcttaacaaa atgtggcagt caaatacttg ctacagtttc agttttggct
    16261 gttaaaggca gtttggctcc aatatctgga acagttcaaa gtgctcatct tattataaga
    16321 tttgacgaaa atggagtgct actaaacaat tccttcctgg acccagaata ttggaacttt
    16381 agaaatggag atcttactga aggcacagcc tatacaaacg ctgttggatt tatgcctaac
    16441 ctatcagctt atccaaaatc tcacggtaaa actgccaaaa gtaacattgt cagtcaagtt
    16501 tacttaaacg gagacaaaac taaacctgta acactaacca ttacactaaa cggtacacag
    16561 gaaacaggag acacaactcc aagtgcatac tctatgtcat tttcatggga ctggtctggc
    16621 cacaactaca ttaatgaaat atttgccaca tcctcttaca ctttttcata cattgcccaa
    16681 gaataaagaa tcgtttgtgt tatgtttcaa cgtgtttatt tttcaattgc agaaaatttc
    16741 aagtcatttt tcattcagta gtatagcccc accaccacat agcttataca gatcaccgta
    16801 ccttaatcaa actcacagaa ccctagtatt caacctgcca cctccctccc aacacacaga
    16861 gtacacagtc ctttctcccc ggctggcctt aaaaagcatc atatcatggg taacagacat
    16921 attcttaggt gttatattcc acacggtttc ctgtcgagcc aaacgctcat cagtgatatt
    16981 aataaactcc ccgggcagct cacttaagtt catgtcgctg tccagctgct gagccacagg
    17041 ctgctgtcca acttgcggtt gcttaacggg cggcgaagga gaagtccacg cctacatggg
    17101 ggtagagtca taatcgtgca tcaggatagg gcggtggtgc tgcagcagcg cgcgaataaa
    17161 ctgctgccgc cgccgctccg tcctgcagga atacaacatg gcagtggtct cctcagcgat
    17221 gattcgcacc gcccgcagca taaggcgcct tgtcctccgg gcacagcagc gcaccctgat
    17281 ctcacttaaa tcagcacagt aactgcagca cagcaccaca atattgttca aaatcccaca
    17341 gtgcaaggcg ctgtatccaa agctcatggc ggggaccaca gaacccacgt ggccatcata
    17401 ccacaagcgc aggtagatta agtggcgacc cctcataaac acgctggaca taaacattac
    17461 ctcttttggc atgttgtaat tcaccacctc ccggtaccat ataaacctct gattaaacat
    17521 ggcgccatcc accaccatcc taaaccagct ggccaaaacc tgcccgccgg ctatacactg
    17581 cagggaaccg ggactggaac aatgacagtg gagagcccag gactcgtaac catggatcat
    17641 catgctcgtc atgatatcaa tgttggcaca acacaggcac acgtgcatac acttcctcag
    17701 gattacaagc tcctcccgcg ttagaaccat atcccaggga acaacccatt cctgaatcag
    17761 cgtaaatccc acactgcagg gaagacctcg cacgtaactc acgttgtgca ttgtcaaagt
    17821 gttacattcg ggcagcagcg gatgatcctc cagtatggta gcgcgggttt ctgtctcaaa
    17881 aggaggtaga cgatccctac tgtacggagt gcgccgagac aaccgagatc gtgttggtcg
    17941 tagtgtcatg ccaaatggaa cgccggacgt agtcatattt cctgaagcaa aaccaggtgc
    18001 gggcgtgaca aacagatctg cgtctccggt ctcgccgctt agatcgctct gtgtagtagt
    18061 tgtagtatat ccactctctc aaagcatcca ggcgccccct ggcttcgggt tctatgtaaa
    18121 ctccttcatg cgccgctgcc ctgataacat ccaccaccgc agaataagcc acacccagcc
    18181 aacctacaca ttcgttctgc gagtcacaca cgggaggagc gggaagagct ggaagaacca
    18241 tgtttttttt tttattccaa aagattatcc aaaacctcaa aatgaagatc tattaagtga
    18301 acgcgctccc ctccggtggc gtggtcaaac tctacagcca aagaacagat aatggcattt
    18361 gtaagatgtt gcacaatggc ttccaaaagg caaacggccc tcacgtccaa gtggacgtaa
    18421 aggctaaacc cttcagggtg aatctcctct ataaacattc cagcaccttc aaccatgccc
    18481 aaataattct catctcgcca ccttctcaat atatctctaa gcaaatcccg aatattaagt
    18541 ccggccattg taaaaatctg ctccagagcg ccctccacct tcagcctcaa gcagcgaatc
    18601 atgattgcaa aaattcaggt tcctcacaga cctgtataag attcaaaagc ggaacattaa
    18661 caaaaatacc gcgatcccgt aggtcccttc gcagggccag ctgaacataa tcgtgcaggt
    18721 ctgcacggac cagcgcggcc acttccccgc caggaaccat gacaaaagaa cccacactga
    18781 ttatgacacg catactcgga gctatgctaa ccagcgtagc cccgatgtaa gcttgttgca
    18841 tgggcggcga tataaaatgc aaggtgctgc tcaaaaaatc aggcaaagcc tcgcgcaaaa
    18901 aagaaagcac atcgtagtca tgctcatgca gataaaggca ggtaagctcc ggaaccacca
    18961 cagaaaaaga caccattttt ctctcaaaca tgtctgcggg tttctgcata aacacaaaat
    19021 aaaataacaa aaaaacattt aaacattaga agcctgtctt acaacaggaa aaacaaccct
    19081 tataagcata agacggacta cggccatgcc ggcgtgaccg taaaaaaact ggtcaccgtg
    19141 attaaaaagc accaccgaca gctcctcggt catgtccgga gtcataatgt aagactcggt
    19201 aaacacatca ggttgattca catcggtcag tgctaaaaag cgaccgaaat agcccggggg
    19261 aatacatacc cgcaggcgta gagacaacat tacagccccc ataggaggta taacaaaatt
    19321 aataggagag aaaaacacat aaacacctga aaaaccctcc tgcctaggca aaatagcacc
    19381 ctcccgctcc agaacaacat acagcgcttc cacagcggca gccataacag tcagccttac
    19441 cagtaaaaaa gaaaacctat taaaaaaaca ccactcgaca cggcaccagc tcaatcagtc
    19501 acagtgtaaa aaagggccaa gtgcagagcg agtatatata ggactaaaaa atgacgtaac
    19561 ggttaaagtc cacaaaaaac acccagaaaa ccgcacgcga acctacgccc agaaacgaaa
    19621 gccaaaaaac ccacaacttc ctcaaatcgt cacttccgtt ttcccacgtt acgtcacttc
    19681 ccattttaag aaaactacaa ttcccaacac atacaagtta ctccgcccta aaacctacgt
    19741 cacccgcccc gttcccacgc cccgcgccac gtcacaaact ccaccccctc attatcatat
    19801 tggcttcaat ccaaaataag gtatattatt gatgatgtta attaagaatt cggatctgcg
    19861 acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg
    19921 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
    19981 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    20041 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    20101 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    20161 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    20221 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    20281 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    20341 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    20401 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    20461 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    20521 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    20581 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    20641 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    20701 aaaaggatct tcacctagat ccttttaaat caatctaaag tatatatgag taaacttggt
    20761 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    20821 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    20881 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    20941 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    21001 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    21061 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    21121 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    21181 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    21241 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    21301 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    21361 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
    21421 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    21481 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    21541 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    21601 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    21661 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    21721 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
    21781 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaatt
//
