LOCUS Exported 10251 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Reporter of Erk activity. ACCESSION . VERSION . KEYWORDS ErkKTR-irFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10251) AUTHORS Dine E, Gil AA, Uribe G, Brangwynne CP, Toettcher JE TITLE Protein Phase Separation Provides Long-Term Memory of Transient Spatial Stimuli. JOURNAL Cell Syst. 2018 May 24. pii: S2405-4712(18)30190-X. doi: 10.1016/j.cels.2018.05.002. PUBMED 29859829 REFERENCE 2 (bases 1 to 10251) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10251 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2050..2167 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2320..2727 /label=SFFV promoter /note="spleen focus-forming virus long terminal repeat (LTR) promoter" regulatory 3064..3073 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3070..4017 /codon_start=1 /product="phytochrome-based near-infrared fluorescent protein, originally termed iRFP (Filonov et al., 2011)" /label=iRFP713 /note="name was changed to iRFP713 (Shcherbakova and Verkhusha, 2013)" /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE" misc_feature 4115..4703 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4168..4188) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4586..4597) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(4706..4722) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4707..4723) /label=pBluescriptKS /note="For pBluescript vector" LTR 4813..5046 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" promoter complement(5071..5089) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(5072..5089) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(5379..5398) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(5612..5631) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 5731..5753 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(5791..5809) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5877..5981 /gene="bla" /label=AmpR promoter CDS 5982..6842 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6200..6219) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7013..7601 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7502..7521 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7755..7772 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(7842..7862) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7847..8176 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 8027..8162 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8089..8108 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 9310..9375 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 9505..9525 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 9950..10084 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9987..10006) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10041..10060 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt 2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca 2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat 2221 cgaattcctg cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga 2281 atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca 2341 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag 2401 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa 2461 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga 2521 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg 2581 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt 2641 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca 2701 gtcctccgac agactgagtc gcccgggggg gatctggagc tctcgagaat tctcacgcgt 2761 ggctccacca tgaagggccg aaagcctcgg gacctggaac ttccacttag cccaagcctg 2821 ctgggtggcc agggacctga acggactcca ggatcaggaa caagctctgg tcttcaggca 2881 ccggggccag cgctttcgcc cagcaagcgt agcggtctcg aggacccagc gacgccatcg 2941 aagaagccac gtaccccatc ggtgtcgtca aggctggagc gcctgaccct ccagtccagc 3001 tttcaattcc catccactag tacgcgtcaa gtggagcaag gcaggtggac agtggatccg 3061 gtcgccacca tggctgaagg atccgtcgcc aggcagcctg acctcttgac ctgcgacgat 3121 gagccgatcc atatccccgg tgccatccaa ccgcatggac tgctgctcgc cctcgccgcc 3181 gacatgacga tcgttgccgg cagcgacaac cttcccgaac tcaccggact ggcgatcggc 3241 gccctgatcg gccgctctgc ggccgatgtc ttcgactcgg agacgcacaa ccgtctgacg 3301 atcgccttgg ccgagcccgg ggcggccgtc ggagcaccga tcactgtcgg cttcacgatg 3361 cgaaaggacg caggcttcat cggctcctgg catcgccatg atcagctcat cttcctcgag 3421 ctcgagcctc cccagcggga cgtcgccgag ccgcaggcgt tcttccgccg caccaacagc 3481 gccatccgcc gcctgcaggc cgccgaaacc ttggaaagcg cctgcgccgc cgcggcgcaa 3541 gaggtgcgga agattaccgg cttcgatcgg gtgatgatct atcgcttcgc ctccgacttc 3601 agcggcgaag tgatcgcaga ggatcggtgc gccgaggtcg agtcaaaact aggcctgcac 3661 tatcctgcct caaccgtgcc ggcgcaggcc cgtcggctct ataccatcaa cccggtacgg 3721 atcattcccg atatcaatta tcggccggtg ccggtcaccc cagacctcaa tccggtcacc 3781 gggcggccga ttgatcttag cttcgccatc ctgcgcagcg tctcgcccgt ccatctggaa 3841 ttcatgcgca acataggcat gcacggcacg atgtcgatct cgattttgcg cggcgagcga 3901 ctgtggggat tgatcgtttg ccatcaccga acgccgtact acgtcgatct cgatggccgc 3961 caagcctgcg agctagtcgc ccaggttctg gcctggcaga tcggcgtgat ggaagaggcg 4021 gccgcaactc ccacctgcaa catgcgtgac tgactgaggc cgcgactcta gagtcgacct 4081 gcaggcatgc aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg 4141 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 4201 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 4261 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 4321 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 4381 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 4441 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 4501 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg 4561 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 4621 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 4681 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagggaatt aattcgagct 4741 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 4801 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt 4861 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 4921 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc 4981 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 5041 ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat 5101 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa 5161 ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag 5221 agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat 5281 ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt 5341 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga 5401 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt 5461 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc 5521 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca 5581 taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 5641 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 5701 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 5761 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta 5821 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg 5881 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 5941 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 6001 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 6061 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 6121 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 6181 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 6241 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 6301 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 6361 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 6421 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 6481 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 6541 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 6601 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 6661 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 6721 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 6781 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 6841 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 6901 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 6961 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 7021 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 7081 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 7141 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 7201 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 7261 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 7321 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 7381 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 7441 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 7501 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 7561 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 7621 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 7681 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 7741 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac 7801 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg 7861 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 7921 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 7981 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 8041 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 8101 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 8161 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac 8221 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac 8281 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt 8341 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct 8401 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat 8461 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga 8521 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg 8581 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga 8641 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt 8701 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat 8761 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat 8821 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag 8881 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc 8941 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc 9001 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc 9061 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa 9121 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat 9181 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt 9241 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa 9301 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt 9361 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc 9421 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact 9481 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac 9541 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct 9601 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa 9661 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt 9721 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc 9781 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact 9841 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc 9901 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat 9961 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 10021 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 10081 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat 10141 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt 10201 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t //