LOCUS Exported 644 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Flp recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.. ACCESSION . VERSION . KEYWORDS pCAFNF-DsRed SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 644) AUTHORS Matsuda T, Cepko CL TITLE Controlled expression of transgenes introduced by in vivo electroporation. JOURNAL Proc Natl Acad Sci U S A. 2007 Jan 5. ():. PUBMED 17209010 REFERENCE 2 (bases 1 to 644) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..644 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 12..45 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" primer_bind complement(142..161) /label=Neo-R /note="Neomycin resistance gene, reverse primer" ORIGIN 1 agcgaacgcg tgaagttcct attctctaga aagtatagga acttccggat ttgatctgat 61 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct 121 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc 181 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc 241 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc 301 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg 361 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag 421 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc 481 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt 541 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc 601 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgt //