LOCUS Exported 5769 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Membrane Adaptor, Excitation: 505 / 569, Emission: 516 / 581. ACCESSION . VERSION . KEYWORDS mEos2-Homer1-N-18 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5769) TITLE Davidson Photoactivatable Fluorescent Proteins REFERENCE 2 (bases 1 to 5769) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5769 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..204 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 155..175 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 233..242 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1391..2071 /codon_start=1 /product="monomeric variant of green-to-red photoswitchable fluorescent protein EosFP (McKinney et al., 2009)" /label=mEos2 /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD NARR" polyA_signal 2193..2314 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2230..2249) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2284..2303 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2321..2776) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2458..2479) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2670..2689 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2803..2907 /gene="bla" /label=AmpR promoter promoter 2909..3266 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2932..2952) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3117..3252 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3179..3198 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3301..4095 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3355..3374) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3965..3984 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4283..4302) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4327..4374 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4703..5291 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5192..5211 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 5466..5769 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgccaccat 241 gggggaacaa cctatcttca gcactcgagc tcatgtcttc cagatcgacc caaacacaaa 301 gaagaactgg gtacccacca gcaagcatgc agttactgtg tcttatttct atgacagcac 361 aaggaatgtg tataggataa tcagtttaga cggctcaaag gcaataataa atagcaccat 421 cactccaaac atgacattta ctaaaacatc tcaaaagttt ggccaatggg ctgatagccg 481 ggcaaacact gtttatggac tgggattctc ctctgagcat catctctcaa aatttgcaga 541 aaagtttcag gaatttaaag aagctgctcg gctggcaaag gagaagtcgc aggagaagat 601 ggaactgacc agtacccctt cacaggaatc agcaggagga gatcttcagt ctcctttaac 661 accagaaagt atcaatggga cagatgatga gagaacaccc gatgtgacac agaactcaga 721 gccaagggct gagccagctc agaatgcatt gccattttca catagtgccg gggatcgaac 781 ccagggcctc tctcatgcta gttcagccat cagcaaacac tgggaggctg aactagccac 841 gctcaagggg aacaatgcca agctcaccgc agcgctgctg gagtccactg ccaacgtgaa 901 gcagtggaag caacagctgg ctgcctacca ggaggaggca gagcggctgc acaagcgggt 961 cacggagctg gaatgtgtta gtagtcaagc aaacgcggtg cacagccaca agacagagct 1021 gagtcagaca gtgcaggagc tggaagagac cctaaaagta aaggaagagg aaatagaaag 1081 attaaaacaa gaaattgata acgccagaga acttcaagaa cagagggact ctttgactca 1141 gaaactacag gaagttgaga ttcgaaataa agacctggag gggcagctgt cggagctgga 1201 gcagcgcctg gagaagagcc agagcgagca ggacgctttc cgcagtaacc tgaagactct 1261 cctagagatt ctggacggga aaatatttga actaacagaa ttgcgggata atttggccaa 1321 gctactagaa tgcagcgggt ccagcggttc tagcggctcc gggagtggag gggatccacc 1381 ggtcgccacc atgagtgcga ttaagccaga catgaagatc aaactccgta tggaaggcaa 1441 cgtaaacggg caccactttg tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa 1501 acagagtatg gatcttgaag tcaaagaggg cggacctctg ccttttgcct ttgatatcct 1561 gaccactgca ttccattacg gcaacagggt attcgccaaa tatccagaca acatacaaga 1621 ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga 1681 cgggggcatt tgcattgcca gaaacgacat aacaatggaa ggggacactt tctataataa 1741 agttcgattt tatggtacca actttcccgc caatggtcca gttatgcaga agaagacgct 1801 gaaatgggag ccctccactg agaaaatgta tgtgcgtgat ggagtgctga cgggtgatat 1861 tcatatggct ttgttgcttg aaggaaatgc ccattaccga tgtgacttca gaactactta 1921 caaagctaag gagaagggtg tcaagttacc aggctaccac tttgtggacc actgcattga 1981 gattttaagc catgacaaag attacaacaa ggttaagctg tatgagcatg ctgttgctca 2041 ttctggattg cctgacaatg ccagacgata agcggccgcg actctagatc ataatcagcc 2101 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 2161 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 2221 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 2281 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt 2341 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 2401 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 2461 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 2521 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 2581 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 2641 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 2701 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 2761 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 2821 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 2881 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt 2941 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 3001 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 3061 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 3121 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 3181 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 3241 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc 3301 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 3361 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 3421 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 3481 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 3541 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 3601 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 3661 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 3721 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 3781 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 3841 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 3901 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 3961 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 4021 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 4081 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc 4141 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 4201 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 4261 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg 4321 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac 4381 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc 4441 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag 4501 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac 4561 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 4621 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 4681 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 4741 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 4801 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 4861 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 4921 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 4981 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 5041 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 5101 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 5161 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 5221 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 5281 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 5341 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca 5401 tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 5461 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 5521 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 5581 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 5641 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 5701 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 5761 attaccatg //