LOCUS Exported 7017 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION fluorescent fusion protein. ACCESSION . VERSION . KEYWORDS IKK2-EYFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7017) AUTHORS Birbach A, Gold P, Binder BR, Hofer E, de Martin R, Schmid JA TITLE Signaling molecules of the NF-kappa B pathway shuttle constitutively between cytoplasm and nucleus. JOURNAL J Biol Chem. 2002 Mar 29;277(13):10842-51. doi: 10.1074/jbc.M112475200. Epub 2002 Jan 18. PUBMED 11801607 REFERENCE 2 (bases 1 to 7017) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7017 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 54..357 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 358..561 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 512..532 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 2893..2902 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2899..3615 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2944..2965) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3205..3224) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3552..3573 /label=EGFP-C /note="EGFP, forward primer" misc_feature 3616..3681 /label=MCS /note="multiple cloning site" polyA_signal 3798..3919 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3835..3854) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3889..3908 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(3926..4381) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4063..4084) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4275..4294 /label=F1ori-R /note="F1 origin, reverse primer" promoter 4408..4512 /gene="bla" /label=AmpR promoter promoter 4514..4871 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(4537..4557) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4722..4857 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4784..4803 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4906..5700 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4960..4979) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5570..5589 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(5888..5907) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5932..5979 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 6308..6896 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6797..6816 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 61 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 121 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 181 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 241 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 301 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 361 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 421 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 481 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 541 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagac tcgacttcga 601 tatctcgaat tcgatatgag ctggtcacct tccctgacaa cgcagacatg tggggcctgg 661 gaaatgaaag agcgccttgg gacaggggga tttggaaatg tcatccgatg gcacaatcag 721 gaaacaggtg agcagattgc catcaagcag tgccggcagg agctcagccc ccggaaccga 781 gagcggtggt gcctggagat ccagatcatg agaaggctga cccaccccaa tgtggtggct 841 gcccgagatg tccctgaggg gatgcagaac ttggcgccca atgacctgcc cctgctggcc 901 atggagtact gccaaggagg agatctccgg aagtacctga accagtttga gaactgctgt 961 ggtctgcggg aaggtgccat cctcaccttg ctgagtgaca ttgcctctgc gcttagatac 1021 cttcatgaaa acagaatcat ccatcgggat ctaaagccag aaaacatcgt cctgcagcaa 1081 ggagaacaga ggttaataca caaaattatt gacctaggat atgccaagga gctggatcag 1141 ggcagtcttt gcacatcatt cgtggggacc ctgcagtacc tggccccaga gctactggag 1201 cagcagaagt acacagtgac cgtcgactac tggagcttcg gcaccctggc ctttgagtgc 1261 atcacgggct tccggccctt cctccccaac tggcagcccg tgcagtggca ttcaaaagtg 1321 cggcagaaga gtgaggtgga cattgttgtt agcgaagact tgaatggaac ggtgaagttt 1381 tcaagctctt taccctaccc caataatctt aacagtgtcc tggctgagcg actggagaag 1441 tggctgcaac tgatgctgat gtggcacccc cgacagaggg gcacggatcc cacgtatggg 1501 cccaatggct gcttcaaggc cctggatgac atcttaaact taaagctggt tcatatcttg 1561 aacatggtca cgggcaccat ccacacctac cctgtgacag aggatgagag tctgcagagc 1621 ttgaaggcca gaatccaaca ggacacgggc atcccagagg aggaccagga gctgctgcag 1681 gaagcgggcc tggcgttgat ccccgataag cctgccactc agtgtatttc agacggcaag 1741 ttaaatgagg gccacacatt ggacatggat cttgtttttc tctttgacaa cagtaaaatc 1801 acctatgaga ctcagatctc cccacggccc caacctgaaa gtgtcagctg tatccttcaa 1861 gagcccaaga ggaatctcgc cttcttccag ctgaggaagg tgtggggcca ggtctggcac 1921 agcatccaga ccctgaagga agattgcaac cggctgcagc agggacagcg agccgccatg 1981 atgaatctcc tccgaaacaa cagctgcctc tccaaaatga agaattccat ggcctccatg 2041 tctcagcagc tcaaggccaa gttggatttc ttcaaaacca gcatccagat tgacctggag 2101 aagtacagcg agcaaaccga gtttgggatc acatcagata aactgctgct ggcctggagg 2161 gaaatggagc aggctgtgga gctctgtggg cgggagaacg aagtgaaact cctggtagaa 2221 cggatgatgg ctctgcagac cgacattgtg gacttacaga ggagtcccat gggccggaag 2281 caggggggaa cgctggacga cctagaggag caagcaaggg agctgtacag gagactaagg 2341 gaaaaacctc gagaccagcg aactgagggt gacagtcagg aaatggtacg gctgctgctt 2401 caggcaattc agagcttcga gaagaaggtg cgagtgatct atacgcagct cagtaaaact 2461 gtggtttgca agcagaaggc gctggaactg ttgcccaagg tggaagaggt ggtgagctta 2521 atgaatgagg atgagaagac tgttgtccgg ctgcaggaga agcggcagaa ggagctctgg 2581 aatctcctga agattgcttg tagcaaggtc cgtggtcctg tcagtggaag cccggatagc 2641 atgaatgcct ctcgacttag ccagcctggg cagctgatgt ctcagccctc cacggcctcc 2701 aacagcttac ctgagccagc caagaagagt gaagaactgg tggctgaagc acataacctc 2761 tgcaccctgc tagaaaatgc catacaggac actgtgaggg aacaagacca gagtttcacg 2821 gccctagact ggagctggtt acagacggaa gaagaagagc acagctgcct ggagcagggg 2881 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 2941 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 3001 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 3061 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc 3121 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 3181 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 3241 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 3301 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 3361 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 3421 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 3481 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag 3541 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 3601 gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac 3661 ggtaccgcgg gcccgggatc caccggatct agatcataat cagccatacc acatttgtag 3721 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 3781 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 3841 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 3901 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg 3961 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct 4021 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt 4081 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat 4141 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca 4201 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac 4261 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta 4321 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg 4381 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 4441 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 4501 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg 4561 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 4621 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 4681 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 4741 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 4801 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 4861 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 4921 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 4981 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 5041 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg 5101 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 5161 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 5221 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 5281 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 5341 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 5401 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc 5461 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 5521 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 5581 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 5641 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 5701 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 5761 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 5821 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga 5881 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa 5941 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc 6001 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt 6061 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg 6121 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa 6181 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 6241 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 6301 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 6361 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 6421 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 6481 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 6541 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 6601 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 6661 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 6721 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 6781 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 6841 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 6901 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 6961 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttat //