LOCUS       Exported                 815 bp ds-DNA     linear   SYN 19-JUL-2021
DEFINITION  To overexpress Thioredoxin-1.
ACCESSION   .
VERSION     .
KEYWORDS    pblic-neo TRX1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Samaranayake GJ, Troccoli CI, Huynh M, Lyles RDZ, Kage K, Win A, 
            Lakshmanan V, Kwon D, Ban Y, Chen SX, Zarco ER, Jorda M, Burnstein 
            KL, Rai P
  TITLE     Thioredoxin-1 protects against androgen receptor-induced redox 
            vulnerability in castration-resistant prostate cancer.
  JOURNAL   Nat Commun. 2017 Oct 31;8(1):1204. doi: 10.1038/s41467-017-01269-x.
  PUBMED    29089489
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      9..18
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(357..377)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        362..691
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      542..677
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     604..623
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 ttctcacggc caccatggtg aagcagatcg agagcaagac tgcttttcag gaagccttgg
       61 acgctgcagg tgataaactt gtagtagttg acttctcagc cacgtggtgt gggccttgca
      121 aaatgatcaa gcctttcttt cattccctct ctgaaaagta ttccaacgtg atattccttg
      181 aagtagatgt ggatgactgt caggatgttg cttcagagtg tgaagtcaaa tgcatgccaa
      241 cattccagtt ttttaagaag ggacaaaagg tgggtgaatt ttctggagcc aataaggaaa
      301 agcttgaagc caccattaat gaattagtct aacgtggaat gtgagctcga ccctgtggaa
      361 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      421 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
      481 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
      541 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
      601 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
      661 aggctttttt ggaggcctag gcttttgcaa aaagcttcac gctgccgcaa gcactcaggg
      721 cgcaagggct gctaaaggaa gcggaacacg tagaaagcca gtccgcagaa acggtgctga
      781 ccccggatga atgtcagcta ctgggctatc tggga
//
