LOCUS Exported 5369 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pEGFP VAMP7 (1-220) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5369) AUTHORS Martinez-Arca S, Alberts P, Zahraoui A, Louvard D, Galli T TITLE Role of tetanus neurotoxin insensitive vesicle-associated membrane protein (TI-VAMP) in vesicular transport mediating neurite outgrowth. JOURNAL J Cell Biol. 2000 May 15;149(4):889-900. PUBMED 10811829 REFERENCE 2 (bases 1 to 5369) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5369 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 94..397 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 398..601 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 552..572 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 640..649 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 646..1362 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 646..1362 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(691..712) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(952..971) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1299..1320 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 2190..2311 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2227..2246) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2281..2300 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2318..2773) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2455..2476) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2667..2686 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2800..2904 /gene="bla" /label=AmpR promoter promoter 2906..3263 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2929..2949) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3114..3249 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3176..3195 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3298..4092 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3352..3371) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3962..3981 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4280..4299) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4324..4371 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4700..5288 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5189..5208 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg 61 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 121 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 181 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 241 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga 301 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg 361 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat 421 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 481 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc 541 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 601 tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc 661 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 721 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 781 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 841 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 901 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 961 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 1021 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 1081 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac 1141 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 1201 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 1261 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 1321 accgccgccg ggatcactct cggcatggac gagctgtaca agtactcaga tctcgagctc 1381 aagcttcgaa ttctgatggc gattcttttt gctgttgttg ccagggggac cactatcctt 1441 gccaaacatg cttggtgtgg aggaaacttc ctggaggtga cagagcagat tctggctaag 1501 ataccttctg aaaataacaa actaacgtac tcacatggca attatttgtt tcattacatc 1561 tgccaagaca ggattgtata tctttgtatc actgatgatg attttgaacg ttcccgagcc 1621 tttaattttc tgaatgagat aaagaagagg ttccagacta cttacggttc aagagcacag 1681 acagcacttc catatgccat gaatagcgag ttctcaagtg tcttagctgc acagctgaag 1741 catcactctg agaataaggg cctagacaaa gtgatggaga ctcaagccca agtggatgaa 1801 ctgaaaggaa tcatggtcag aaacatagat ctggtagctc agcgaggaga aagattggaa 1861 ttattgattg acaaaacaga aaatcttgtg gattcttctg tcaccttcaa aactaccagc 1921 agaaatcttg ctcgagccat gtgtatgaag aacctcaagc tcactattat catcatcatc 1981 gtatcaattg tgttcatcta tatcattgtt tcacctctct gtggtggatt tacatggcca 2041 agctgtgtga agaaatagcg ggatccaccg gatctagata actgatcata atcagccata 2101 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga 2161 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca 2221 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 2281 gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg 2341 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 2401 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 2461 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 2521 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 2581 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 2641 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 2701 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 2761 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 2821 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 2881 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc 2941 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 3001 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 3061 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 3121 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 3181 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 3241 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg 3301 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 3361 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 3421 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa 3481 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 3541 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 3601 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 3661 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 3721 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 3781 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc 3841 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 3901 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 3961 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 4021 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 4081 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc 4141 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 4201 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 4261 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta 4321 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg 4381 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat 4441 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc 4501 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt 4561 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 4621 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 4681 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 4741 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 4801 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 4861 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 4921 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 4981 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 5041 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 5101 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 5161 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 5221 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 5281 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 5341 ctcacatgtt ctttcctgcg ttatcccct //